Molecular dynamics simulation reveals how phosphorylation of tyrosine 26 of phosphoglycerate mutase 1 upregulates glycolysis and promotes tumor growth

Wang, Yan; Cai, Wensheng; Chen, Luonan; Wang, Guanyu

doi:10.18632/oncotarget.14517

Cited by 17 publications

(13 citation statements)

References 46 publications

(51 reference statements)

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“…PGAM catalyzes the conversion of 3-phosphoglycerate to 2-phosphoglycerate, which is a crucial step in glycolysis. Previous phosphoproteomics studies showed that PGAM can be phosphorylated at multiple tyrosine sites, which enhances PGAM activity and upregulates glycolysis [41, 42]. In this study, phosphorylated PGAM was down-regulated only in C101LAC 12 h after SA treatment.…”

Section: Discussionsupporting

confidence: 46%

Comparative phosphoproteomic analysis of blast resistant and susceptible rice cultivars in response to salicylic acid

et al. 2019

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BackgroundSalicylic acid (SA) is a significant signaling molecule that induces rice resistance against pathogen invasion. Protein phosphorylation carries out an important regulatory function in plant defense responses, while the global phosphoproteome changes in rice response to SA-mediated defense response has not been reported. In this study, a comparative phosphoproteomic profiling was conducted by two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) analysis, with two near-isogenic rice cultivars after SA treatment.ResultsThirty-seven phosphoprotein spots were differentially expressed after SA treatment, twenty-nine of which were identified by MALDI-TOF/TOF MS, belonging to nine functional categories. Phosphoproteins involved in photosynthesis, antioxidative enzymes, molecular chaperones were similarly expressed in the two cultivars, suggesting SA might alleviate decreases in plant photosynthesis, regulate the antioxidant defense activities, thus improving basal resistance response in both cultivars. Meanwhile, phosphoproteins related to defense, carbohydrate metabolism, protein synthesis and degradation were differentially expressed, suggesting phosphorylation regulation mediated by SA may coordinate complex cellular activities in the two cultivars. Furthermore, the phosphorylation sites of four identified phosphoproteins were verified by NanoLC-MS/MS, and phosphorylated regulation of three enzymes (cinnamoyl-CoA reductase, phosphoglycerate mutase and ascorbate peroxidase) was validated by activity determination.ConclusionsOur study suggested that phosphorylation regulation mediated by SA may contribute to the different resistance response of the two cultivars. To our knowledge, this is the first report to measure rice phosphoproteomic changes in response to SA, which provides new insights into molecular mechanisms of SA-induced rice defense.

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Section: Discussionsupporting

confidence: 46%

Comparative phosphoproteomic analysis of blast resistant and susceptible rice cultivars in response to salicylic acid

et al. 2019

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“…Thus, simulations are expected to be effective for increasingly more complex systems. Larger number of atoms and longer simulation time frames become accessible to reproduce realistic binding/unbinding events that may occur in biosensor systems [ 120 , 121 , 130 , 131 , 132 , 133 ]. Recently, complete fully atomistic models of capsids such as of HIV-1 and Hepatitis-B viruses were developed [ 134 , 135 , 136 ], which may facilitate much more accurate modeling of interaction events at biosensor/capsid interfaces.…”

Section: Computational Approaches To Lc Sensor Designsmentioning

confidence: 99%

Thermotropic Liquid Crystal-Assisted Chemical and Biological Sensors

et al. 2017

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In this review article, we analyze recent progress in the application of liquid crystal-assisted advanced functional materials for sensing biological and chemical analytes. Multiple research groups demonstrate substantial interest in liquid crystal (LC) sensing platforms, generating an increasing number of scientific articles. We review trends in implementing LC sensing techniques and identify common problems related to the stability and reliability of the sensing materials as well as to experimental set-ups. Finally, we suggest possible means of bridging scientific findings to viable and attractive LC sensor platforms.

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“…The promoting roles of PGAM1 in aggressive tumors have received increasing attention in recent years. Several studies have suggested that PGAM1 promoted tumor growth by upregulating the glycolysis process 9,10 . In a research from Hitosugi et al 11 , the phosphorylation of PGAM1 (Y26) induced by oncogenic tyrosine kinases has been shown to activate its activity and promote tumor growth.…”

Section: Introductionmentioning

confidence: 99%

Phosphoglycerate Mutase 1 (PGAM1) Promotes Pancreatic Ductal Adenocarcinoma (PDAC) Metastasis by Acting as a Novel Downstream Target of the PI3K/Akt/mTOR Pathway

et al. 2018

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Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive tumors known, with an overall 5-year survival rate of less than 6% due to early local invasion and distant metastasis. Exploring suitable therapeutic targets associated with invasion and metastasis is required for improving the prognosis of PDAC. In this study, we investigated the role of the glycolytic enzyme phosphoglycerate mutase 1 (PGAM1) in PDAC. PGAM1 expression was examined in tissue samples of 54 PDAC patients using immunohistochemistry, and the correlation between clinicopathological expression and PGAM1 expression was determined. A survival curve was generated using the Kaplan-Meier method. After silencing PGAM1 by siRNA in pancreatic cancer cell lines Aspc-1 and Panc-1, the changes in proliferation, migration, and invasion, and signal pathways were determined. In this study, the expression of PGAM1 was found positively related to poor differentiation, metastasis, advanced clinical stage, and poor survival rate. Silencing PGAM1 decreased the proliferation of Aspc-1 and Panc-1 cells with an S phase arrest, but without influencing cell apoptosis. Migration and invasion also decreased significantly, independent of proliferation. PGAM1 was also found to promote EMT of PDAC cell lines by regulating the Wnt/β-catenin pathway. PGAM1 itself was modulated by the PI3K/Akt/mTOR pathway as a novel downstream target and has a positive mutual regulation with HIF-1α. This study indicates that PGAM1 is closely associated with clinical metastasis and poor prognosis of PDAC. PGAM1 is considered as a potential therapeutic target in PDAC metastasis.

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Molecular dynamics simulation reveals how phosphorylation of tyrosine 26 of phosphoglycerate mutase 1 upregulates glycolysis and promotes tumor growth

Cited by 17 publications

References 46 publications

Comparative phosphoproteomic analysis of blast resistant and susceptible rice cultivars in response to salicylic acid

Comparative phosphoproteomic analysis of blast resistant and susceptible rice cultivars in response to salicylic acid

Thermotropic Liquid Crystal-Assisted Chemical and Biological Sensors

Phosphoglycerate Mutase 1 (PGAM1) Promotes Pancreatic Ductal Adenocarcinoma (PDAC) Metastasis by Acting as a Novel Downstream Target of the PI3K/Akt/mTOR Pathway

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