Molecular docking and spectral shift supported toxicity profile of metaldehyde mollucide and the toxicity-reducing effects of bitter melon extract

Macar, Oksal; Macar, Tuğçe Kalefetoğlu; Yalçın, Emine; Çavuşoğlu, Kültiğin; Acar, Ali

doi:10.1016/j.pestbp.2022.105201

Cited by 11 publications

(7 citation statements)

References 51 publications

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“…For this purpose, DNA was isolated from A. cepa root tip cells and then used for spectral measurements. DNA isolation from Allium root samples was carried out according to the method suggested by Macar et al 8 . DNA–MnCl 2 interaction was evaluated by investigating the change in absorbance of DNA and DNA–MnCl 2 mixtures (1:1, 1:2, 1:3).…”

Section: Methodsmentioning

confidence: 99%

Screening the toxicity profile and genotoxicity mechanism of excess manganese confirmed by spectral shift

Tümer

Çavuşoğlu

Yalçın

2022

Sci Rep

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In this study, the toxicity induced by excessive doses of manganese (MnCl2), which is one of the essential trace elements for the continuation of the metabolic activities of the organisms, was investigated with the help of the Allium test. Toxicity was investigated by using physiological (percent germination, root length, weight gain), cytogenetic [mitotic index (MI), micronucleus (MN), chromosomal abnormalities (CAs)], biochemical [malondialdehyde (MDA), superoxide dismutase (SOD) catalase (CAT)] and anatomical (root tip meristematic cell damage) parameters. Allium cepa L. bulbs were divided into four groups as one control and three treatments. The control group was germinated with tap water, and the treatment groups were germinated with 250, 500 and 1000 µM doses of MnCl2. The germination process was continued for 72 h without interruption. At the end of the period, the root tips were collected, washed in distilled water and made ready for microscopic and spectrophotometric analyzes with the help of routine preparation techniques. As a result, the highest germination percentage, root length, weight gain and MI, and the lowest MN frequency, CAs numbers, MDA level, SOD and CAT enzyme activities were determined in the control group (group I). MnCl2 exposure caused a decrease in physiological parameter values and an increase in cytogenetic (except MI) and biochemical parameter values, depending on the dose. MnCl2 exposure induced MN and CAs such as fragment, sticky chromosome, vagrant chromosome, unequal distribution of chromatin and bridge. This genotoxic effect of MnCl2 was associated with DNA–MnCl2 interaction, and this interaction was also confirmed by bathochromic and hypochromic shifts in spectral analysis. Anatomical damages such as epidermis cell damage, flattened cell nucleus, cortex cell damage and cortex cell wall thickening were observed after MnCl2 treatment. As a result, it has been determined that excessive doses of the trace element Mn cause physiological, cytogenetic, biochemical and anatomical toxicity and A. cepa test material is a reliable bio-indicator in determining this toxicity.

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Section: Methodsmentioning

confidence: 99%

Screening the toxicity profile and genotoxicity mechanism of excess manganese confirmed by spectral shift

Tümer

Çavuşoğlu

Yalçın

2022

Sci Rep

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“…To gain insight into the role of cyclic(alkyl)(amino)carbene (CAAC) as well as the NHC complex of ligands, CuCl was used as an accelerator in the Nmethylation reaction.Nevertheless, 1a was attained only in a low yield of 7 % (entry 12). These results suggest that CAAC and NHC ligands play a dominant role in the selective generation of N-methylation products (entries [6][7][8][9][10][11][12]. A dominant role of CAAC and NHC ligands in the selective generation of Nmethylation products is suggested by some of these results (entries [6][7][8][9][10][11][12].…”

Section: Table 12mentioning

confidence: 76%

“…These results suggest that CAAC and NHC ligands play a dominant role in the selective generation of N-methylation products (entries [6][7][8][9][10][11][12]. A dominant role of CAAC and NHC ligands in the selective generation of Nmethylation products is suggested by some of these results (entries [6][7][8][9][10][11][12]. The authors also explored the effect of reducing agents (e. g., PhSiH 3 , Ph 2 SiH 2 , and (EtO) 3 SiH), which showed 9b yields of 99 %, 74 %, and 82 %, whereas the use of Ph 3 SiH as a reducing agent resulted in a 9b yield of only 15 %.…”

Section: Table 12mentioning

confidence: 90%

C−N Bond Formation Using Paraformaldehyde as C1 Source – Recent Advances

Guo,

Wu,

Wei

2023

Asian J Org Chem

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Recently, polyformaldehyde (PFA) has been used as C1 source to synthesize a series of value‐added compounds due to its low price, stability, low level of toxicity and easy operation. Exploiting paraformaldehyde as C1 source, rather than using traditional methylation reagent to develop benign N‐methylation reactions, can be more environment‐friendly and make the chemical industry more sustainable. In this paper, the chemical transformation of polyformaldehyde and a new strategy of methylating polyformaldehyde with amine compound to methylamine compound are reviewed. These methods will provide a reference for researchers to synthesize a large number of methylamine compounds in a short reaction by using paraformaldehyde as a single carbon source.

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“…The flattened nucleus arises as a result of changes in the physiological, biochemical and DNA integrity of the cell nucleus. The cell nucleus is typically spherical or elliptical in nature, but shape change can occur in response to physical or environmental changes 71 , 72 . All these changes observed in the anatomical structure of the groups treated with N. oleander leaf and flowers emerge as a result of cumulative effects.…”

Section: Resultsmentioning

confidence: 99%

Qualitative and quantitative phytochemical screening of Nerium oleander L. extracts associated with toxicity profile

Çilesizoğlu

Yalçın

Çavuşoğlu

et al. 2022

Sci Rep

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In this study, phytochemical analysis and toxicity profile of leaf and flower extracts of Nerium oleander L. species collected from Giresun province (Turkey) were investigated. In phytochemical analyzes, the cardiac glycoside, alkaloid, saponin and tannin contents of the extracts were analyzed qualitatively and quantitatively. The physiological effects of extracts were determined by examining root elongation, weight gain and germination rates. Biochemical effects were determined by measuring the levels of malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT), which are indicators of oxidative stress. Cytotoxic and genotoxic effects were investigated by mitotic index (MI), micronucleus (MN) and chromosomal abnormality (CA) tests. N. oleander leaf and flower extract applications caused significant decreases in the physiological parameters of Allium bulbs. SOD and CAT activity in root tip cells increased significantly after the application of leaf extract compared to the control group. Similar changes were observed in the application of flower extract, but these increases were found to be at a lower level compared to the increases induced by the leaf extract. An increase in MDA levels and a decrease in GSH levels were observed in groups treated with leaf and flower extracts. These changes show that the extracts cause deterioration in antioxidant/oxidant balance. It was determined that the extracts, which caused a decrease in MI rates and an increase in MN and CAs frequencies, showed the most prominent cytotoxic and genotoxic effects at 250 μg/mL doses. These toxic effects were associated with the phytochemical content of the extracts, and it was thought that cardiac glycosides and alkaloids, whose presence were detected in qualitative and quantitative analyzes, may play an important role in toxicity. Studies investigating the therapeutic properties of plants as well as their toxic effects are insufficient, which leads to the fact that plants exhibiting potential toxicity are not well known. Therefore, this study will lead many studies on the toxicity profile of the phytochemical contents of plants. Therefore, this study will draw attention to the investigation of the toxicity profile and phytochemical contents of plants and will lead to similar studies.

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Molecular docking and spectral shift supported toxicity profile of metaldehyde mollucide and the toxicity-reducing effects of bitter melon extract

Cited by 11 publications

References 51 publications

Screening the toxicity profile and genotoxicity mechanism of excess manganese confirmed by spectral shift

Screening the toxicity profile and genotoxicity mechanism of excess manganese confirmed by spectral shift

C−N Bond Formation Using Paraformaldehyde as C1 Source – Recent Advances

Qualitative and quantitative phytochemical screening of Nerium oleander L. extracts associated with toxicity profile

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