Molecular Diversity of the CoI–CoII Spacer Region in the Mitochondrial Genome and the Origin of the Carpathian Bee

Cherevatov, O. V.; Панчук, І. І.; Kerek, Stepan; Волков, Р. А.

doi:10.3103/s0095452719040030

Cited by 16 publications

(11 citation statements)

References 21 publications

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“…Identification of honeybee haplotype diversity. The comparative analysis of the mitochondrial DNA (mtDNA) variance in the COI-COII region is extensively used to identify the honeybee subspecies and genetic diversity (53). For each gut DNA sample, the COI-COII region of mtDNA of honeybee was amplified with the primer pair prC1C2-L (59-CCACGACGTTATTCAGACTATCCA-39) and prC1C2-R (59-CAT ATGATCAATATCATTGATGACCAA-39) (53).…”

Section: Methodsmentioning

confidence: 99%

“…The comparative analysis of the mitochondrial DNA (mtDNA) variance in the COI-COII region is extensively used to identify the honeybee subspecies and genetic diversity (53). For each gut DNA sample, the COI-COII region of mtDNA of honeybee was amplified with the primer pair prC1C2-L (59-CCACGACGTTATTCAGACTATCCA-39) and prC1C2-R (59-CAT ATGATCAATATCATTGATGACCAA-39) (53). PCR was carried out in 50-ml reaction mixtures containing 2 U Taq DNA polymerase (TaKaRa, Tokyo, Japan), 5 ml dNTPs (2.5 mM), 1 ml each primer (5 mM), and 10 ng template DNA.…”

Section: Methodsmentioning

confidence: 99%

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Host Species and Geography Differentiate Honeybee Gut Bacterial Communities by Changing the Relative Contribution of Community Assembly Processes

Jing

Diao

et al. 2021

mBio

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Host Species and Geography Differentiate Honeybee Gut Bacterial Communities by Changing the Relative Contribution of Community Assembly Processes

Jing

Diao

et al. 2021

mBio

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“…A total of 1696 sequences from neighbouring countries and Serbia originating from different time periods and deposited in NCBI GenBank [ 12 , 24 , 33 , 36 , 39 , 41 , 44 , 45 , 46 , 47 , 48 , 49 ] were used for comparison ( Table S2 ).…”

Section: Methodsmentioning

confidence: 99%

“…Approximately five worker bees from the apiary were chosen for genetic analysis, each representing one beehive. A total of 1696 sequences from neighbouring countries and Serbia originating from different time periods and deposited in NCBI GenBank [12,24,33,36,39,41,[44][45][46][47][48][49] were used for comparison (Table S2).…”

Section: Samplingmentioning

confidence: 99%

“…After amplification, the PCR products were purified according to [51], digested with restriction enzymes NcoI and StyI and then visualized on 2% agarose gel stained with ethidium bromide under UV light. Expected A total of 1696 sequences from neighbouring countries and Serbia originating from different time periods and deposited in NCBI GenBank [12,24,33,36,39,41,[44][45][46][47][48][49] were used for comparison (Table S2).…”

Section: Dna Extraction and Pcr-rflp Analysismentioning

confidence: 99%

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MtDNA Analysis Indicates Human-Induced Temporal Changes of Serbian Honey Bees Diversity

Tanasković

Erić

Patenković

et al. 2021

Insects

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Local populations of Apis mellifera are rapidly changing by modern beekeeping through the introduction of nonnative queens, selection and migratory beekeeping. To assess the genetic diversity of contemporary managed honey bees in Serbia, we sequenced mitochondrial tRNAleu-cox2 intergenic region of 241 worker bees from 46 apiaries at eight localities. Nine haplotypes were observed in our samples, with C2d being the most common and widespread. To evaluate genetic diversity patterns, we compared our data with 1696 sequences from the NCBI GenBank from neighbouring countries and Serbia. All 32 detected haplotypes belonged to the Southeast Europe lineage C, with two newly described haplotypes from our sample. The most frequent haplotype was C2d, followed by C2c and C1a. To distinguish A. m. carnica from A. m. macedonica, both previously reported in Serbia, PCR-RFLP analysis on the COI gene segment of mtDNA was used, and the result showed only the presence of A.m. carnica subspecies. An MDS plot constructed on pairwise FST values showed significant geographical stratification. Our samples are grouped together, but distant from the Serbian dataset from the GenBank. This, with the absence of A. m. macedonica subspecies from its historic range of distribution in southern Serbia, indicates that honey bee populations are changing rapidly due to the anthropogenic influence.

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A reply to the Letter to the Editor of Moškrič et al. entitled “A comment on the paper from Utzeri et al. (2022) “Entomological authentication of honey based on a DNA method that distinguishes Apis mellifera mitochondrial C mitotypes: Application to honey produced by A. m. ligustica and A. m. carnica, Food control, Volume 121, March 2021, 107626”.

2023

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Molecular Diversity of the CoI–CoII Spacer Region in the Mitochondrial Genome and the Origin of the Carpathian Bee

Cited by 16 publications

References 21 publications

Host Species and Geography Differentiate Honeybee Gut Bacterial Communities by Changing the Relative Contribution of Community Assembly Processes

Host Species and Geography Differentiate Honeybee Gut Bacterial Communities by Changing the Relative Contribution of Community Assembly Processes

MtDNA Analysis Indicates Human-Induced Temporal Changes of Serbian Honey Bees Diversity

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