Host Species and Geography Differentiate Honeybee Gut Bacterial Communities by Changing the Relative Contribution of Community Assembly Processes

Ge, Yuan; Jing, Zhongwang; Diao, Qingyun; He, Ji‐Zheng; Liu, Yongjun

doi:10.1128/mbio.00751-21

Cited by 44 publications

(56 citation statements)

References 58 publications

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“…This partly explains why the fungal networks from Capniidae species exhibited more interconnection compared to other stonefly families. In congruence with recent work that revealed the effect of geographic variables on ecological processes 33 , our results also find a significant link between fungal community assembly and environmental factors such as altitude, latitude, longitude, AMT, and AP. Thus, the strength of ecological processes driving fungal communities towards either divergence or convergence is dependent on environmental factors and associated evolutionary history.…”

Section: Discussionsupporting

confidence: 92%

“…Variable selection may result in the microbiota communities diversifying among distinct environmental conditions, whereas drift disperses communities 7 , 36 , 53 . Drift was the main stochastic process driving stonefly fungal communities in our study, a similar result to reports on bacterial communities in the honeybee 33 . The core fungal genus Aspergillus found in the wild stoneflies in our work has also been detected in other insects 39 , 40 , and some of its members are also found in the surrounding environment to the host, such as in the air, soil, and on plants 54 .…”

Section: Discussionsupporting

confidence: 91%

“…The relative contribution of each ecological process varies among different host taxa and different environments 19 , 29 – 31 . For instance, strong effects of deterministic forces on gut microbial community composition have been reported in studies on wild Drosophila 32 , while neutrality is dominant over selection in the differentiation of the gut bacterial community in honeybees 33 . As these works demonstrate, the microbial community assembly in terrestrial insects has received some attention 32 – 35 .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Mechanisms of fungal community assembly in wild stoneflies moderated by host characteristics and local environment

Zhu

Huo

Wen

et al. 2022

npj Biofilms Microbiomes

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Deterministic and stochastic forces both drive microbiota assembly in animals, yet their relative contribution remains elusive, especially in wild aquatic-insect-associated fungal communities. Here, we applied amplicon sequencing to survey the assembly mechanisms of the fungal community in 155 wild stonefly individuals involving 44 species of 20 genera within eight families collected from multiple locations in China. Analysis showed that fungal diversity and network complexity differed significantly among the eight stonefly families, and that the fungal communities in stoneflies exhibited a significant distance-decay pattern across large spatial scales. Both a structural equation model and variance partitioning analysis revealed that environmental factors (e.g., geographical, climatic) outweigh host attributes in shaping the fungal community of stoneflies. Using neutral and null model analyses, we also find that deterministic processes play a larger role than stochasticity in driving the fungal community assembly. However, the relative contribution of ecological processes including dispersal, drift, and selection, varied strongly with host taxonomy. Furthermore, environmental conditions also significantly affect the strength of these ecological processes. Overall, our findings illustrate that variations in host attributes and environment factors may moderate the relative influence of deterministic and stochastic processes to fungal community composition in wild stoneflies, which provides new insights into mechanisms of microbial community assembly in aquatic arthropods.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mechanisms of fungal community assembly in wild stoneflies moderated by host characteristics and local environment

Zhu

Huo

Wen

et al. 2022

npj Biofilms Microbiomes

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“…But, it is still unclear whether, and how, REO NPs affect gut microbiota of honeybees, specifically the model species Apis mellifera . Honeybee gut microbiota could be influenced by various factors, including pathogens, antibiotics, pesticides, diet, and host attributes and environmental conditions ( Cox-Foster et al, 2007 ; Raymann et al, 2017 ; Liu et al, 2019 ; Ge et al, 2021 ). Previous study has demonstrated that the shifts of gut bacterial communities in bumblebees may serve as a characteristic of disease states, featuring as low abundance of core species and high incidence of opportunistic environmental bacteria ( Cariveau et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Nano-La2O3 Induces Honeybee (Apis mellifera) Death and Enriches for Pathogens in Honeybee Gut Bacterial Communities

et al. 2021

Self Cite

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Honeybees (Apis mellifera) can be exposed via numerous potential pathways to ambient nanoparticles (NPs), including rare earth oxide (REO) NPs that are increasingly used and released into the environment. Gut microorganisms are pivotal in mediating honeybee health, but how REO NPs may affect honeybee health and gut microbiota remains poorly understood. To address this knowledge gap, honeybees were fed pollen and sucrose syrup containing 0, 1, 10, 100, and 1000mgkg−1 of nano-La2O3 for 12days. Nano-La2O3 exerted detrimental effects on honeybee physiology, as reflected by dose-dependent adverse effects of nano-La2O3 on survival, pollen consumption, and body weight (p<0.05). Nano-La2O3 caused the dysbiosis of honeybee gut bacterial communities, as evidenced by the change of gut bacterial community composition, the enrichment of pathogenic Serratia and Frischella, and the alteration of digestion-related taxa Bombella (p<0.05). There were significant correlations between honeybee physiological parameters and the relative abundances of pathogenic Serratia and Frischella (p<0.05), underscoring linkages between honeybee health and gut bacterial communities. Taken together, this study demonstrates that nano-La2O3 can cause detrimental effects on honeybee health, potentially by disordering gut bacterial communities. This study thus reveals a previously overlooked effect of nano-La2O3 on the ecologically and economically important honeybee species Apis mellifera.

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“…The gut microbial composition of honeybees is simpler than that of humans ( Kwong and Moran 2016 ). The honeybee gut harbors a diverse microbial community, among which lactic acid bacteria (LAB) and bifidobacteria are beneficial in the intestine ( Ge et al 2021 ). On the contrast, some pathogenic bacteria such as Paenibacillus larvae and Melissococcus pluton have been found to cause diseases in honeybees ( Fünfhaus et al 2018 ).…”

Section: Introductionmentioning

confidence: 99%

In vitro Effects of Prebiotics and Synbiotics on Apis cerana Gut Microbiota

Lei

Yin

et al. 2021

Polish Journal of Microbiology

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This study aimed to investigate in vitro effects of the selected pre-biotics alone, and in combination with two potential probiotic Lactobacillus strains on the microbial composition of Apis ceranagut microbiota and acid production. Four prebiotics, inulin, fructo-oligosaccharides, xylo-oligosaccharides, and isomalto-oligosac¬charides were chosen, and glucose served as the carbon source. Supplementation of this four prebiotics increased numbers of Bifidobacterium and lactic acid bacteria while decreasing the pH value of in vitro fermentation broth inoculated with A. cerana gut microbiota compared to glucose. Then, two potential probiot¬ics derived from A. cerana gut at different dosages, Lactobacillus helveticus KM7 and Limosilactobacillus reuteri LP4 were added with isomalto-oligosaccharides in fermentation broth inoculated with A. cerana gut microbiota, respectively. The most pronounced impact was observed with isomalto-oligosaccharides. Compared to isomalto-oligosaccharides alone, the combination of isomalto-oligo¬saccharides with both lactobacilli strains induced the growth of Bifi¬dobacterium, LAB, and total bacteria and reduced the proliferation of Enterococcus and fungi. Consistent with these results, the altered metabolic activity was observed as lowered pH in in vitro culture of gut microbiota supplemented with isomalto-oligosaccharides and lactobacilli strains. The symbiotic impact varied with the typesand concentration of Lactobacillus strains and fermentation time. The more effective ability was observed with IMO combined with L. helveticus KM7. These results suggested that isomalto-oligosac¬charides could be a potential prebiotic and symbiotic with certain lactobacilli strains on A. cerana gut microbiota.

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Host Species and Geography Differentiate Honeybee Gut Bacterial Communities by Changing the Relative Contribution of Community Assembly Processes

Abstract: Honeybees provide crucial pollination services and valuable apiarian products. The symbiotic intestinal communities facilitate honeybee health and fitness by promoting nutrient assimilation, detoxifying toxins, and resisting pathogens.

Cited by 44 publications

References 58 publications

Mechanisms of fungal community assembly in wild stoneflies moderated by host characteristics and local environment

Mechanisms of fungal community assembly in wild stoneflies moderated by host characteristics and local environment

Nano-La2O3 Induces Honeybee (Apis mellifera) Death and Enriches for Pathogens in Honeybee Gut Bacterial Communities

In vitro Effects of Prebiotics and Synbiotics on Apis cerana Gut Microbiota

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