Molecular Dissection of Endosomal Compartments in Plants

Müller, Jens; Mettbach, Ursula; Menzel, Diedrik; Šamaj, Jozef

doi:10.1104/pp.107.102863

Cited by 47 publications

(41 citation statements)

References 113 publications

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“…S6 in the supplementary material) (von der Fecht-Bartenbach et al, 2007). In plants, accumulating evidence exists for a unified TGN/EE compartment (Dettmer et al, 2006;Müller et al, 2007;Robinson et al, 2008). These results suggest that NEV is localized at the TGN/EE.…”

Section: Nev Localizes To the Tgn And Endosomesmentioning

confidence: 74%

Regulation of membrane trafficking and organ separation by the NEVERSHED ARF-GAP protein

et al. 2009

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Cell separation, or abscission, is a highly specialized process in plants that facilitates remodeling of their architecture and reproductive success. Because few genes are known to be essential for organ abscission, we conducted a screen for mutations that alter floral organ shedding in Arabidopsis. Nine recessive mutations that block shedding were found to disrupt the function of an ADP-ribosylation factor-GTPase-activating protein (ARF-GAP) we have named NEVERSHED (NEV). As predicted by its homology to the yeast Age2 ARF-GAP and transcriptional profile, NEV influences other aspects of plant development, including fruit growth. Colocalization experiments carried out with NEV-specific antiserum and a set of plant endomembrane markers revealed that NEV localizes to the trans-Golgi network and endosomes in Arabidopsis root epidermal cells. Interestingly, transmission electron micrographs of abscission zone regions from wild-type and nev flowers reveal defects in the structure of the Golgi apparatus and extensive accumulation of vesicles adjacent to the cell walls. Our results suggest that NEV ARF-GAP activity at the trans-Golgi network and distinct endosomal compartments is required for the proper trafficking of cargo molecules required for cell separation.

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Section: Nev Localizes To the Tgn And Endosomesmentioning

confidence: 74%

Regulation of membrane trafficking and organ separation by the NEVERSHED ARF-GAP protein

et al. 2009

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“…In addition, FLY1-YFP does not resemble the reticular distribution of endoplasmic reticulum or the linear distribution of plasma membrane (PM) markers. Thus, FLY1-YFP most likely resides in a compartment between the TGN/EE and the PM, namely, the LE/MVB/PVC and/or the vacuole (Müller et al, 2007). Although FM4-64 and Brefeldin A were not taken up by seed coat epidermal cells, we successfully treated seeds with wortmannin, which induces the fusion of LE/MVB/PVC in plant cells (Wang et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

FLYING SAUCER1 Is a Transmembrane RING E3 Ubiquitin Ligase That Regulates the Degree of Pectin Methylesterification inArabidopsisSeed Mucilage

et al. 2013

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Pectins are complex polysaccharides that form the gel matrix of the primary cell wall and are abundant in the middle lamella that holds plant cells together. Their degree of methylesterification (DM) impacts wall strength and cell adhesion since unesterified pectin regions can cross-link via Ca 2+ ions to form stronger gels. Here, we characterize flying saucer1 (fly1), a novel Arabidopsis thaliana seed coat mutant, which displays primary wall detachment, reduced mucilage extrusion, and increased mucilage adherence. These defects appear to result from a lower DM in mucilage and are enhanced by the addition of Ca 2+ or completely rescued using alkaline Ca 2+ chelators. FLY1 encodes a transmembrane protein with a RING-H2 domain that has in vitro E3 ubiquitin ligase activity. FLY1 is orthologous to TRANSMEMBRANE UBIQUITIN LIGASE1, a Golgi-localized E3 ligase involved in the quality control of membrane proteins in yeast. However, FLY1-yellow fluorescent protein (YFP) fusions are localized in punctae that are predominantly distinct from the Golgi and the trans-Golgi network/early endosome in the seed coat epidermis. Wortmannin treatment, which induces the fusion of late endosomes in plants, resulted in enlarged FLY1-YFP bodies. We propose that FLY1 regulates the DM of pectin in mucilage, potentially by recycling pectin methylesterase enzymes in the endomembrane system of seed coat epidermal cells.

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“…In the past years, several investigations documented TGN as both a secretory organelle and an early endosomal compartment (e.g. Dettmer et al, 2006;Dhonukshe et al, 2006;Lam et al, 2007;Mü ller et al, 2007;Tahara et al, 2007). Both TGN and PM are sites of clathrin-coated vesicle formation (Barth and Holstein, 2004).…”

Section: Mpk6 Associates With Pm and Tgn Vesiclesmentioning

confidence: 99%

Arabidopsis MPK6 is involved in cell division plane control during early root development, and localizes to the pre-prophase band, phragmoplast, trans-Golgi network and plasma membrane

et al. 2009

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). † These two authors contributed equally to this work. SUMMARYThe proper spatial and temporal expression and localization of mitogen-activated protein kinases (MAPKs) is essential for developmental and cellular signalling in all eukaryotes. Here, we analysed expression, subcellular localization and function of MPK6 in roots of Arabidopsis thaliana using wild-type plants and three mpk6 knock-out mutant lines. The MPK6 promoter showed two expression maxima in the most apical part of the root meristem and in the root transition zone. This expression pattern was highly consistent with 'no root' and 'short root' phenotypes, as well as with ectopic cell divisions and aberrant cell division planes, resulting in disordered cell files in the roots of these mpk6 knock-out mutants. In dividing root cells, MPK6 was localized on the subcellular level to distinct fine spots in the pre-prophase band and phragmoplast, representing the two most important cytoskeletal structures controlling the cell division plane. By combining subcellular fractionation and microscopic in situ and in vivo co-localization methods, MPK6 was localized to the plasma membrane (PM) and the trans-Golgi network (TGN). In summary, these data suggest that MPK6 localizing to mitotic microtubules, secretory TGN vesicles and the PM is involved in cell division plane control and root development in Arabidopsis.

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Molecular Dissection of Endosomal Compartments in Plants

Cited by 47 publications

References 113 publications

Regulation of membrane trafficking and organ separation by the NEVERSHED ARF-GAP protein

Regulation of membrane trafficking and organ separation by the NEVERSHED ARF-GAP protein

FLYING SAUCER1 Is a Transmembrane RING E3 Ubiquitin Ligase That Regulates the Degree of Pectin Methylesterification inArabidopsisSeed Mucilage

Arabidopsis MPK6 is involved in cell division plane control during early root development, and localizes to the pre-prophase band, phragmoplast, trans-Golgi network and plasma membrane

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