Molecular dissection of cholinesterase domains responsible for carbamate toxicity

Loewenstein, Yael; Denarie, Michel; Zakut, Haim; Soreq, Hermona

doi:10.1016/0009-2797(93)90044-y

Cited by 15 publications

(4 citation statements)

References 7 publications

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“…With regard to serum inhibition, the calculated IC 30 value and IC 50 value were in the range of 48ȫg L -1 and 80ȫg L -1 . The IC 50 value range was quite similar to measurements obtained via Ellman's method [40,41], as the inhibition response varies within samples, indicating that, like Ellman's method, our platform was not influenced by carbamate inhibition.…”

Section: Diluted Ache Can Be Distinguished On Our Platformsupporting

confidence: 68%

Evaluating organophosphate poisoning in human serum with paper

Yen

Chen

Hsu

et al. 2015

Talanta

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Section: Diluted Ache Can Be Distinguished On Our Platformsupporting

confidence: 68%

Evaluating organophosphate poisoning in human serum with paper

Yen

Chen

Hsu

et al. 2015

Talanta

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“…pounds has been documented in other studies Ripley and Chau, 1982;Loewenstein et al, 1993;Chen et al, . 1994 , this effect has not been demonstrated for DTCs.…”

supporting

confidence: 56%

“…Their use has been increasingly associated with genotoxic, immunotoxic, and cyto-Ž toxic effects Frakes, 1988;Oskarsson and Hellstrom-Lindahl, 1988;Kalayanova and Ivanova-Chemishanska, . 1989;Padgett et al, 1992;Scarabelli et al, 1993 . Carbamate insecticides and many of their derived compounds are well known to inhibit acetylcholines-Ž terase Ripley and Chau, 1982;Loewenstein et al, . 1993;Chen et al, 1994 , but DTCs have not been shown to have the same effect, at least not to a significant degree.…”

mentioning

confidence: 99%

In vitro evaluation of the toxicity of several dithiocarbamates using anEscherichia coli growth inhibition bioassay and the acetylcholinesterase inhibition test

Espigares¹,

Crovetto²,

Gálvez³

1998

Environ. Toxicol. Water Qual.

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The industrial and sanitary use of dithiocarbamates DTCs is on the rise, and the synthesis of new derivatives has increased the field of their application. Both positive and negative pharmacological and toxicological effects have been documented for DTCs. The development and application of new 50 exhibited a minimal inhibitory effect on AChE, the results obtained indicate a generalized absence of AChE inhibition for the DTCs.

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“…In contrast to the medical uses of AChE inhibitors [4], which reversibly inhibit AChE, toxic molecules, such as insecticides, usually act through irreversible inhibition [5,6]. Carbamate chemicals, which are commonly used in various insecticides, have been widely studied for their AChE inhibition [5,7] and environmental toxicity to vertebrates [8]. The high toxicity of Electronic supplementary material The online version of this article (doi:10.1007/s00396-015-3648-y) contains supplementary material, which is available to authorized users.…”

Section: Introductionmentioning

confidence: 99%

Liquid crystal sensor for the detection of acetylcholine using acetylcholinesterase immobilized on a nanostructured polymeric surface

Han

Jang

2015

Colloid Polym Sci

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In this study, we report on a label-free biosensor for the detection of acetylcholine (ACh) and an acetylcholinesterase (AChE) inhibitor, utilizing liquid crystals (LCs) on a polymeric surface with periodic nanostructures. The polymeric nanostructures, which hold sinusoidal anisotropic patterns, were fabricated by a sequential process of poly(dimethylsiloxane) buckling and replication of these patterns on a poly(urethane acrylate) surface where a film of gold was deposited. AChE was then covalently immobilized onto the gold surface. Optical images of nematic 4-cyano-4′-pentylbiphenyl (5CB) revealed that it aligned parallel to the plane of the enzymedecorated surface. However, AChE-mediated hydrolysis of ACh resulted in a plume of choline, acetate, and unreacted ACh, which in turn produced a distinctive optical transition of 5CB (from uniform to random) by masking the anisotropic surface topography. The hydrolysis of ACh was inhibited in the presence of a carbamate insecticide (AChE inhibitor), which prevented the orientational transition of 5CB by decreasing the enzymatic activity of AChE. These results suggest that this LC-based enzymatic sensor is highly sensitive to ACh and the AChE inhibitor, with a detection limit of 10 and 1 nM, respectively. This study demonstrates that polymeric surfaces with continuous wavy features can be used as LCbased biosensors to amplify the existence of ACh and an AChE inhibitor without labeling or an additional amplification strategy.

show abstract

Molecular dissection of cholinesterase domains responsible for carbamate toxicity

Cited by 15 publications

References 7 publications

Evaluating organophosphate poisoning in human serum with paper

Evaluating organophosphate poisoning in human serum with paper

In vitro evaluation of the toxicity of several dithiocarbamates using anEscherichia coli growth inhibition bioassay and the acetylcholinesterase inhibition test

Liquid crystal sensor for the detection of acetylcholine using acetylcholinesterase immobilized on a nanostructured polymeric surface

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