Evaluating organophosphate poisoning in human serum with paper

Yen, Tzung‐Hai; Chen, Kuan‐Hung; Hsu, Min‐Yen; Fan, Shuting; Huang, Yishuo; Chang, Chia‐Ling; Wang, Yuping; Cheng, Chao‐Min

doi:10.1016/j.talanta.2015.05.049

Cited by 6 publications

(7 citation statements)

References 41 publications

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“…For this experiment, we designed a simple microfluidic pattern using Microsoft PowerPoint software (Microsoft, Redmond, WA, USA)3637. The full length of the pattern is 2.8 mm; the circular diameter is 0.4 mm.…”

Section: Methodsmentioning

confidence: 99%

“…We printed the pattern on chromatography paper (Grade 1 Qualitative Filter Papers, GE Healthcare Life Sciences, Little Chalfont, Bucks, UK), as shown in Fig. 1 using wax-printer (Phaser 8560 wax printer, Xerox, Norwalk, CT, USA)3637. After baking the paper using the patterned wax at 105 °C for 3 min, hydrophilic-hydrophobic regions formed inside the chromatography paper.…”

Section: Methodsmentioning

confidence: 99%

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Relationship between Porcine Sperm Motility and Sperm Enzymatic Activity using Paper-based Devices

Matsuura

Huang

Chen

et al. 2017

Sci Rep

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Mammalian sperm motility has traditionally been analyzed to determine fertility using computer-assisted semen analysis (CASA) systems. To develop low-cost and robust male fertility diagnostics, we created a paper-based MTT assay and used it to estimate motile sperm concentration. When porcine sperm motility was inhibited using sperm enzyme inhibitors for sperm enzymes related to mitochondrial activity and glycolysis, we simultaneously recorded sperm motility and enzymatic reactivity using a portable motility analysis system (iSperm) and a paper-based MTT assay, respectively. When using our paper-based MTT-assay, we calculated the area mean value signal intensity (AMV) to evaluate enzymatic reactivity. Both sperm motility and AMV decreased following treatment with iodoacetamide (IODO) and 3-bromopyruvic acid (3BP), both of which are inhibitors of glycolytic enzymes including glyceraldehyde-3-phosphate dehydrogenase (GAPDH). We found a correlation between recorded motility using iSperm and AMV from our paper-based assay (P < 0.05), suggesting that a sperm-related enzymatic reaction is involved in sperm motility. Under this protocol, MTT reduction was coupled with catalysis of GAPDH and was promoted by electron transfer from NADH. Based on this inhibitor study, sperm motility can be estimated using our paper-based MTT-assay.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Relationship between Porcine Sperm Motility and Sperm Enzymatic Activity using Paper-based Devices

Matsuura

Huang

Chen

et al. 2017

Sci Rep

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“…[45][46][47] Notably, we, and others, have developed PADs to analyze serum organophosphate level for organophosphate poisoning in patients. 48 To the best of our knowledge, few previous studies have examined the use of PADs for clinically diagnostic PQ detection. Herein, we demonstrate the development of a PAD employing a sodium dithionite assay or ascorbic acid assay to determine serum PQ levels of PQ-poisoned patients in only 10 min.…”

Section: Introductionmentioning

confidence: 99%

Paper-based diagnostic devices for clinical paraquat poisoning diagnosis

et al. 2016

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This article unveils the development of a paper-based analytical device designed to rapidly detect and clinically diagnose paraquat (PQ) poisoning. Using wax printing technology, we fabricated a PQ detection device by pattering hydrophobic boundaries on paper. This PQ detection device employs a colorimetric sodium dithionite assay or an ascorbic acid assay to indicate the PQ level in a buffer system or in a human serum system in 10 min. In this test, colorimetric changes, blue in color, were observable with the naked eye. By curve fitting models of sodium dithionite and ascorbic acid assays in normal human serum, we evaluated serum PQ levels for five PQ-poisoned patients before hemoperfusion (HP) treatment and one PQpoisoned patient after HP treatment. As evidenced by similar detection outcomes, the analytical performance of our device can compete with that of the highest clinical standard, i.e., spectrophotometry, with less complicated sample preparation and with more rapid results. Accordingly, we believe that our rapid PQ detection can benefit physicians determining timely treatment strategies for PQ-poisoned patients once they are taken to hospitals, and that this approach will increase survival rates.Published by AIP Publishing. [http://dx

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“…Nevertheless, while most of them focus on organophosphorus compound or carbamate compound detection, few targets PQ detection. [116][117][118] Among these few efforts targeting PQ detection, even fewer efforts deal with PQ detection in human samples. In 2016, Kuan et al 87 introduced a colorimetric paper-based analytical device for PQ detection in human serum.…”

Section: Paper-based Diagnostic Devicementioning

confidence: 99%

“…In a study, 118 we have developed a paper-based, metabolic assay designed for the rapid, semi-quantitative measurement of organophosphate intoxication. The paper-based platforms, including point-of-care devices and 96-well plates, provided semi-quantitative information regarding the concentration of acetylcholinesterase (not individual organophosphate/carbamate compounds).…”

Section: Point-of-care Testing In the Early Diagnosis Of Other Pesticmentioning

confidence: 99%

Point-of-care testing in the early diagnosis of acute pesticide intoxication: The example of paraquat

2018

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Acute pesticide intoxication is a common method of suicide globally. This article reviews current diagnostic methods and makes suggestions for future development. In the case of paraquat intoxication, it is characterized by multi-organ failure, causing substantial mortality and morbidity. Early diagnosis may save the life of a paraquat intoxication patient. Conventional paraquat intoxication diagnostic methods, such as symptom review and urine sodium dithionite assay, are time-consuming and impractical in resource-scarce areas where most intoxication cases occur. Several experimental and clinical studies have shown the potential of portable Surface Enhanced Raman Scattering (SERS), paper-based devices, and machine learning for paraquat intoxication diagnosis. Portable SERS and new SERS substrates maintain the sensitivity of SERS while being less costly and more convenient than conventional SERS. Paper-based devices provide the advantages of price and portability. Machine learning algorithms can be implemented as a mobile phone application and facilitate diagnosis in resource-limited areas. Although these methods have not yet met all features of an ideal diagnostic method, the combination and development of these methods offer much promise.

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Evaluating organophosphate poisoning in human serum with paper

Cited by 6 publications

References 41 publications

Relationship between Porcine Sperm Motility and Sperm Enzymatic Activity using Paper-based Devices

Relationship between Porcine Sperm Motility and Sperm Enzymatic Activity using Paper-based Devices

Paper-based diagnostic devices for clinical paraquat poisoning diagnosis

Point-of-care testing in the early diagnosis of acute pesticide intoxication: The example of paraquat

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