Molecular discrimination of maize CMS type and genetic relationship using RAMs markers

Abstract

Molecular characterization of CMS is an environmentally-independent tool and represents an alternative way to consider genetic variation within and between the different maize populations. The current study which consisted of six cms lines of maize in the 8th generation (S8) revealed that all of these lines were C-type. The adopted six specific markers (forward and reverse) in detecting the three cms types produced a fragment with a molecular size of ∼350 bp. The used RAMs markers (UBC-810, UBC-812, UBC-821, UBC-848, UBC-854 and UBC-855) produced a total of 36 fragments across the lines genomes, 25 out of them were polymorphic scoring a polymorphism percentage of 69.4%. The PIC values indicated that UBC-821 was the most discriminative primer with a PIC value of 0.40 compared to UBC-854, and UBC-855 primers which had the humble capability to distinguish between the studied inbreds with a PIC value of 0.33. The results of cluster analysis based on the molecular discrimination confirmed that A1 has the lowest values of genetic similarity, hence it was the most genetically divergent against the other cms inbred lines, especially A6 (0.42). On the other hand, A2 and A3 lines found to be genetically related by scoring the maximum genetic similarity (0.92). Molecular investigations have several advantages over the traditional methods and will be helpful in the real evaluation of any genotype.

Genetic structure and diversity of Calycolpus moritzianus (Myrtaceae) in the north-eastern Andes of Colombia

“…Our combination of primers produced a similar amount of loci as those previously reported for blackberry Rubus spp. (Morillo et al, 2005), the guava Psidium guajava (Sanabria et al, 2006), the gulupa Passiflora edulis f. edulis (Fonseca et al, 2009), the foraging tree Trichanthera gigantea (Posso et al, 2011), the citrus rootstocks (Gallego et al, 2017), the Moringa oleifera tree (Chaves-Bedoya et al, 2017), the oil palm Elaeis guineensis (Castañeda-Cardona et al, 2018), in Zea mays (Alfalahi et al, 2019), and 5 Narcissus species (Mohammed et al, 2020). Given the absence of molecular studies in C. moritzianus, the number of loci generated by the 5 RAMs primers of this work (n = 81) resulted adequate to estimate genetic parameters and other statistical analyzes (Nybom & Bartish, 2000).…”

“…org/10.22201/ib.20078706e.2021.92.3635 The study of the morphology and genetic variability of plants that can have medicinal and potential commercial purposes, allows for a better use of plant genetic resources (Castañeda-Cardona et al, 2018;Chaves-Bedoya et al, 2017;Palevitch, 1991), and offers important data to understand the ecological and evolutionary processes of natural populations of native trees (Jones & Hubbell, 2006;Mohammed et al, 2020;Staggemeier et al, 2010). Nevertheless, because morphological characters are highly influenced by the environment (phenotypic plasticity) and plant phenology (Arnold et al, 2019;Gianoli & Valladares, 2012), molecular characterizations and the use of DNA markers, which is environmentally independent, are an alternative approach to assess plant genetic variation (Alfalahi et al, 2019;Ansari et al, 2015). The genetic diversity of some Myrtaceae species in South America has been evaluated by using restriction fragment length polymorphisms (AFLP) in Acca sellowiana (Quezada et al, 2014), randomly amplified polymorphic DNA (RAPD) in Eugenia dysenterica (Trindade & Chaves, 2005), and Psidium guajava (Valera-Montero et al, 2016), inter simple sequence repeat (ISSR) in Myrcia lundiana (Alves et al, 2016) and Myrcia ovata (White et al, 2018), and the random amplified microsatellites (RAMs) in Psidium guajava (Sanabria et al, 2006).…”

“…RAMs molecular markers, are a PCR-based method which uses primers containing microsatellite sequences and degenerate anchors at the 5' end (Hantula et al, 1996;Zietkiewicz et al, 1994). RAMs markers are dominant (binary) and multi-allelic and they are an efficient tool to detect and estimate genetic variation in DNA among plants (Alfalahi et al, 2019;Muñoz et al, 2008;Posso et al, 2011;Zietkiewicz et al, 1994).…”

Role of Melatonin Application Date in some Biochemical Characteristics of Sesame Crop Affected by Irrigation Interval

“…Reactive Oxygen Species (ROS) are a natural product of metabolic activities that depend on oxygen, and plants have defense mechanisms against them under normal development conditions (their formation and removal are controlled). When a plant is subjected to environmental stressors, the balance within the cell is significantly impacted, and the production of ROS increases to the point where it places a heavy burden on the plant cell, especially oxidative reactions like hydrogen peroxide, proline, and monaldehyde, some of which take place to get rid of the excessive production of free radicals (ROS) [5,6]. Any substance or product that has an activity against oxidative damage and acts to delay or prevent the action of free radicals is referred to be an antioxidant.…”