Molecular differentiation of Turkey cattle isolates of Fasciola hepatica and Fasciola gigantica

Şimşek, Sami; Ütük, Armağan Erdem; Balkaya, İbrahim

doi:10.2478/s11687-011-0001-y

Cited by 12 publications

(9 citation statements)

References 16 publications

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“…Various lesions can be caused by trematodes in the liver of affected sheep. In F. hepatica infections, cholangiohepatitis, hepatocyte degeneration, micro-abscessation, coagulative necrosis, extensive fibrosis and loss of hepatic parenchyma have been identified (Meeusen et al, 1995;Rushton & Murray, 1997;Kumar, 1999;Simsek et al, 2011). In D. dendriticum infections, hepatocyte damage, cholangitis -cholangiectasia (in the interlobular bile ducts as well), cirrhosis and fibrosis can be present (Jithendran & Bhat, 1996;Kumar, 1999;Manga-González & González-Lanza, 2005).…”

Section: Discussionmentioning

confidence: 99%

Potential association between trematode infections and development of pregnancy toxaemia in sheep

et al. 2013

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Objective of the work was to study a potential association between trematode infections in pregnant ewes and concentrations β-hydroxybutyrate, which is a ketone body found in animals with pregnancy toxaemia. After administration of a long-acting nematocide, 80 pregnant sheep, infected with trematodes, were allocated as follows; primigravidae ewes in group P-A remained untreated and in group P-B were given netobimin; multigravidae ewes in group M-A remained untreated, in group M-B were given netobimin and in group M-C were given rafoxanide. We collected faecal samples for trematode epg counting and blood samples for measurement of β-hydroxybutyrate concentrations. Mean faecal epg counts of D. dendriticum and F. hepatica decreased significantly after netobimin administration; mean faecal epg counts of F. hepatica, but not of D. dendriticum, decreased significantly after rafoxanide administration. Between P-A and P-B, the difference in mean blood β-hydroxybutyrate concentrations was significant (P = 0.036) immediately after lambing. Between M-A or M-C and M-B, it was significant (P ≤ 0.002) 28 days after trematocide administration and immediately after lambing; between M-A and M-C, no significant difference was evident. Immediately after lambing, mean blood β-hydroxybutyrate concentration in primiparous/multiparous ewes with Dicrocoelium faecal output ≤150 epg was 0.21/0.64 mmol L -1 , respectively, and in primiparous/multiparous ewes with Dicrocoelium faecal output >150 epg was 0.40/0.93 mmol L -1 , respectively (P = 0.704/<0.001, respectively). Mean blood β-hydroxybutyrate concentration in group P-C/M-C ewes with Fasciola faecal output of <16/<30 epg was 0.47/0.68 mmol L -1 , respectively; that in group P-C/M-C ewes with Fasciola faecal output of ≥16/≥30 epg was 0.56/0.85 mmol L -1 , respectively (P = 0.620/0.278, respectively). The results indicate that more trematode-infected adult ewes were found to have increased β-hydroxybutyrate blood concentrations and point out to a potential role of liver trematode .... infections in predisposing adult ewes to pregnancy toxaemia.

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Section: Discussionmentioning

confidence: 99%

Potential association between trematode infections and development of pregnancy toxaemia in sheep

et al. 2013

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“…Recent and advanced molecular approaches were utilized to the precise study of Fasciola as a parasite. Most commonly used methods were; Random Amplified Polymorphic DNA (RAPD) (7), single nucleotide polymorphism (SNP) (8), PCR-restricted fragment length polymorphism (PCR-RFLP) (9), and sequencing of the whole genome (10). The distinction between F. hepatica and F. gigantica, two common liver flatworms, in the final hosts, particularly in the cattle and sheep, is crucial because of their diverse epidemiological features.…”

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confidence: 99%

Genotyping study of Fasciola gigantica isolated from cattle in Aqrah city, Iraq

Hamoo¹,

Al-Rubaye²,

Mustafa³

2020

IJVS

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This study was conducted to investigate the 18S rRNA gene of Fasciola gigantica obtained from the liver of cattle live in Aqrah city, Iraq. Fifty-nine Fasciola flukes were collected through routine investigation from livers of naturally infected local cattle (42 cows), from May to August 2017, at the central slaughterhouse of Aqrah city, Kurdistan region of Iraq, the flukes were washed by PBS and then fixed in ethanol. Genomic DNA was extracted, and a 560 bp fragment was amplified by PCR, subsequent by sequencing of PCR products. A remarkable result of this project was the deposition of our gene isolate in GenBank (Accession No. MG786553). However, it was confirmed by the sequence results that isolate species was F. gigantica, and interestingly our samples sequences have alignment match of 100% with many international isolates, without genetic mutations or variations. It is concluded that molecular study could be utilized for both diagnosis and differential diagnosis of parasites with huge precise. Also, an 18S rRNA gene is a perfect fragment for molecular study and phylogenetic analysis of F. gigantica, also our samples have 100% alignment match with universal isolates.

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“…Although fasciolosis commonly occurs in liver and biliary ducts, ec-topic fasciolosis may occur in the peritoneal cavity, lungs, subcutaneous tissue, lymph nodes, eye, and other locations (1, 2). Besides causing disease in humans, it can induce mortality and morbidity in sheep and cattle industry (3).…”

Section: Introductionmentioning

confidence: 99%

“…are the ITSI and ITSII of nuclear DNA and COI from mitochondrial DNA. The COI gene fragment introduced as a most variable marker recommended for future analyses (15,3).…”

Section: Introductionmentioning

confidence: 99%

High-Resolution Melting Analysis as an Appropriate Method to Differentiate between Fasciola hepatica and F. gigantica

Hosseini-Safa¹,

Rokni²,

Mosawi³

et al. 2019

ijph

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Background: Fasciolosis is a shared disease between humans and livestock caused by hepatic trematodes; Fasciola hepatica and F. gigantica. Differentiate between the two species of this genus is essential. High-Resolution Melting (HRM) Analysis represents a new approach to this issue. This method can be performed right after termination of Real-Time PCR. This technique has not been used for identification of adult F. hepatica and F. gigantica genotypes. The aim of this study was to determine Fasciola species by using HRM in isolates taken from Iran, respectively. Methods: Ninety-three Fasciola spp. samples were collected from infected slaughtered animals in different regions of Iran, including North West (Ardebil Province) and South East (Zahedan Province) during 2016. Genomic DNA from the samples was extracted using a DNA extraction kit and then after Real-Time PCR amplification, HRM was done. Results: Overall, 59 and 34 isolates were identified as F. hepatica and F. gigantica, respectively. The percentages of each species from animals were as follows: sheep (F. hepatica, 80.39% and F. gigantica, 19.61%), cattle (F. hepatica, 42.85% and F. gigantica, 57.15%). Conclusion: HRM technique developed in the present study is a powerful, rapid and sensitive technique for epidemiological survey and molecular identification between F. hepatica and F. gigantica.

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Molecular differentiation of Turkey cattle isolates of Fasciola hepatica and Fasciola gigantica

Cited by 12 publications

References 16 publications

Potential association between trematode infections and development of pregnancy toxaemia in sheep

Potential association between trematode infections and development of pregnancy toxaemia in sheep

Genotyping study of Fasciola gigantica isolated from cattle in Aqrah city, Iraq

High-Resolution Melting Analysis as an Appropriate Method to Differentiate between Fasciola hepatica and F. gigantica

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