High-Resolution Melting Analysis as an Appropriate Method to Differentiate between Fasciola hepatica and F. gigantica

Hosseini-Safa, Ahmad; Rokni, Mohammad Bagher; Mosawi, Sayed Hussain; Heydarian, Peyman; Azizi, Hakim; Davari, Afshin; Aryaiepour, Mojgan

doi:10.18502/ijph.v48i3.894

Cited by 10 publications

(9 citation statements)

References 22 publications

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“…The chronic nature of trematode infection can lead to misdiagnosis and consequently the administration of the wrong drugs and exacerbation of the disease ( 16 ). Given the importance of the issue, today, several methods have been designed to increase the sensitivity of diagnostic tests for these diseases ( 17 , 18 ). Human infection with Fasciola sp.…”

Section: Discussionmentioning

confidence: 99%

Contamination of Vector Snails with the Larval Stages of Trematodes in Selected Areas in Northern Iran

Aryaeipour¹,

Mansoorian²,

Rad³

et al. 2022

ijph

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Background: Identification of freshwater snails and possible trematodes transmission sites are essential to continue monitoring the potential for disease outbreaks in areas with a history of parasitic infections. We aimed to search some areas in the margin of the Caspian Sea, northern Iran to identify the snail fauna of this area and verify the contamination of vector snails. Methods: More than 5,308 snails from 51 diverse and permanent habitats were studied from April 2019 to October 2021. Snails were collected randomly and identified using shell morphology. Trematode infection in snails was investigated by the release of cercariae and dissection methods. Results: Five families of freshwater snails including Lymnaeidae, Physidae, Planorbidae, Bithyniidae, and Viviparidae were investigated in the Caspian Sae Litoral of Iran. Physidae were found as the most prevalent snails (55.1%) followed by Lymnaeidae (29.4%). The parasitize rate was observed as 20% using releasing cercaria technique. Echinostomatoidea (31%), Schistosomatoidea (8%), and Diplostomoidea (21%), and Plagiorchioidea (40%) were seen as detected parasites. Meanwhile, 60% of the studied snails illustrated the other stages of trematodes. Conclusion: The rate of infection of snails with different cercaria in northern Iran is significant. It needs further deep studies to clarify the situation of zoonoses transmitted by snails in the region. Policy makers should pay attention more to this area in terms of monitoring the snail-transmitted diseases.

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Section: Discussionmentioning

confidence: 99%

Contamination of Vector Snails with the Larval Stages of Trematodes in Selected Areas in Northern Iran

Aryaeipour¹,

Mansoorian²,

Rad³

et al. 2022

ijph

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“…The results of another study showed that the subjects who were positive for ELISA, their stool and urine samples were 96% and 90% positive by nested PCR (use of ITS1 such as present study) respectively, and only 20% of samples was diagnosed by parasitology method ( 13 ). In addition, using High-Resolution Melting (HRM) there was a powerful, rapid, and sensitive technique for an epidemiological survey and molecular identification between F. hepatica and F. gigantica ( 38 ).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Semi-Nested PCR Compared with Indirect-ELISA to Diagnose Human Fasciolosis

Alizadeh¹,

Aryaeipour²,

Mohebali³

et al. 2022

ijph

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Background: We aimed to compare semi-nested PCR with indirect ELISA to diagnose human fasciolosis. Methods: Overall, 70 serum samples were collected from different areas in Iran suspected for fascioliasis. Individuals were classified based on diagnostic of fascioliasis and habitat in endemic areas. Finally, all serum samples were tested by indirect ELISA (using secretory excretory antigen) and semi-nested PCR (using ITS1 gene). The study was conducted in the School of Publish Health, Tehran University of Medical Sciences, Iran in 2021. Results: Significant differences were found between agreement and similarity of patients' results of indirect ELISA and semi-nested PCR 94.46% and 98.4% respectively (Cohen's kappa ≥0.6; P-value≤0.05). No cross-reactions were observed with other parasitic diseases (toxocariasis, hydatidosis, strongyloidiasis, toxoplasmosis, cutaneous leishmaniasis, taeniasis and trichinosis). 69.84% of samples were positive by both techniques. In addition, the percentage of agreement and similarity between the results of the two techniques based on habitat in endemic areas was 88.9-100% and 97.7-100%, respectively (Cohen's kappa ≥0.6; P-value≤0.05). Conclusion: Semi-nested PCR could be a suitable method for following up on patients' treatment and a confirmatory method for ELISA as for diagnosis of human fascioliasis.

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“…in both human and animal samples ( 19 ). In addition, HRM has been used in parasitic helminth studies such as rapid differentiation between Fasciola hepatica and F. gigantica and also, Echinococcus granulosus and E. canadensis ( 20 , 21 ).…”

Section: Discussionmentioning

confidence: 99%

High-Resolution Melting Analysis in Comparison with Microscopic Method: An Experimental Study to Diagnosis of Plasmodium Species Infections in Human

Allahmoradi

Haghi

Nateghpour

et al. 2020

IJPA

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Background: Among the human parasitic diseases, malaria is the main cause of morbidity and mortality. To prevent the high mortality and tracking malaria elimination efforts, a prompt and sensitive diagnosis is essential. This study aimed to compare High-Resolution Melting (HRM) and microscopic methods to diagnose Plasmodium falciparum and P. vivax. Methods: Eighty-one blood samples were collected from patients with clinical symptoms who were suspect to malaria in Chabahar district, southeastern Iran and also, from those who were referred to Malaria National Laboratory in the Tehran University of Medical Sciences, Tehran, Iran. Microscopic examination and HRM method were used to the diagnosis of Plasmodium parasites simultaneously. Results: Microscopic results revealed 45 positive cases (12 P. falciparum and 33 P. vivax) out of 81 collected samples while according to HRM analysis results 11 and 33 samples were identified as P. falciparum and P. vivax, respectively. HRM analysis also revealed 1 mixed infection of P. falciparum and P. malariae. Conclusion: HRM analysis provides a promising mean for simultaneous detection and discrimination of the Plasmodium spp. especially in mixed infection cases.

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High-Resolution Melting Analysis as an Appropriate Method to Differentiate between Fasciola hepatica and F. gigantica

Cited by 10 publications

References 22 publications

Contamination of Vector Snails with the Larval Stages of Trematodes in Selected Areas in Northern Iran

Contamination of Vector Snails with the Larval Stages of Trematodes in Selected Areas in Northern Iran

Evaluation of Semi-Nested PCR Compared with Indirect-ELISA to Diagnose Human Fasciolosis

High-Resolution Melting Analysis in Comparison with Microscopic Method: An Experimental Study to Diagnosis of Plasmodium Species Infections in Human

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