Molecular differentiation of Thysaniezia (Helictometra) giardi and Moniezia species based on 18s rRNA gene in small ruminants

Alfatlawi, Monyer Abdulameir Abd; Ismail, Yahia Kh.; Ali, Mansour Jadaan; Karawan, Azhar Chafat; Ibadi, Israa N.

doi:10.33899/ijvs.2020.126407.1313

Cited by 5 publications

(4 citation statements)

References 16 publications

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“…The similarity of the current isolates from the present work with the isolates from Kyrgyzstan, Italy, Spain, and Turkey can be explained to some degree due to the moving animals and the flies between countries. It's necessary to know when the parasitic period occure in order to prevent the clinical signs and economic losses caused by O. ovis infection, this knowledge will be a valuable tool to help in choosing the right treatment at the right period (21)(22)(23)(24)(25).…”

Section: Discussionmentioning

confidence: 99%

Morphological and phylogenetic characterization of Oestrus ovis larvae in sheep: Al-Qadisiyah province, Iraq

Alhayali¹,

Alsaadawi²,

Al-Fatlawi³

et al. 2021

IJVS

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The fly larvae infect the nasal cavities and sinuses (frontal and maxillary) of sheep, goats, and a range of wild ruminants, forming a disease called oestrosis (Nasal myiasis or nasal bot). The disease is one of the significantly diseases for the Iraqi small ruminant industry that causes detrimental economic losses. The current work was carried out to morphologically-and molecularly-characterize O. ovis larvae collected from sheep in a slaughterhouse in Al-Qadisiyah province, Iraq. The study depended on collecting 20 larvae (at different stages) from 20 sheep from 15 October till 17 December 2020. The morphological examination was done using a stereomicroscope and relying on larval characteristic features, including the posterior end, spiracles, and cephalopharyngeal skeleton. The molecular characterization was performed utilizing polymerase chain reaction (PCR) and partial gene sequencing (PGS) methods of the cytochrome c oxidase subunit I (cox1) gene at 700bp and 300-bp regions. Morphologically, the first-stage larvae (L1) showed characteristic mouth hooks, while the second-stage larvae (L2) revealed clear terminal stigmas. For the third-stage larvae (L3), the color of body segments and their spines' were the most important features for this larval stage. The PCR showed amplification at both regions 700bp and 300bp, in 8 and 7 isolates, respectively. The PGS revealed 15 different local isolates in genetic level aligned with isolates from Kyrgyzstan, Italy, Spain, and Turkey. This study shows the important strain differences of O. ovis that infect the local sheep in Al-Qadisiyah province, Iraq.

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Section: Discussionmentioning

confidence: 99%

Morphological and phylogenetic characterization of Oestrus ovis larvae in sheep: Al-Qadisiyah province, Iraq

Alhayali¹,

Alsaadawi²,

Al-Fatlawi³

et al. 2021

IJVS

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“…The PCR primers used in this analysis to the amplify ITS2rDNA gene of P. skrjabini, it is being designed according to (9), while COX1mtDNA gene was prepared as (non-degenerate primer) according to the molecular study of other Spirurida (19), these primers were produced from Bioneer company, Korea as following table 1, and reaction conditions of PCR for amplification showed as in the table 2. (20)(21)(22).…”

Section: Methodsmentioning

confidence: 99%

COX1 gene and ITS-2 region: A comparative study of molecular diagnosis of Parabronema skrjabini in camels (Camelus dromedaries), Al-Najaf Province, Iraq

Al-Fatlawi¹,

A-Fatlawy²

2021

IJVS

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The current study was contributed to the analysis of the nucleotide sequence pattern of the nucleotide sequence of the tissue DNA isolates based on the Internal Transcribed Spacer ribosomal DNA (ITS2rDNA) gene and Cytochrome c oxidase subunit 1 mitochondrial DNA (COX1 mtDNA) using the traditional polymerase chain reaction (150 samples of abomasum collected directly from camel carcasses after working in Al-Najaf slaughterhouse from 1/10/2019 to 1/2/2020). ITS2rDNA were well suited for the prepared primer with size783 bp and identical ratio ranged 81.17-99.73% of the same species, as indicated a high similarity of the isolates taken from two-humped camels in China or less related to Parabronema skrjabini in sheep and goat. In addition, the study identified the number of the mutations within the four COX1 gene and ITS-2 region, which were the most conservative region of the host's species, supporting the concept of host specificity with Parabronema skrjabini. The COX1 gene and ITS-2 region applied to confirm the diagnosis using a universal primer, as it included eight isolates with a size of 689 bp, identical values were ranged from 84.99-98.02% depending on the multiple sequence alignment and showed an increase in the substitution level among isolates at an upper taxonomic level. Studying of the COX1 gene and ITS-2 region in Parabronema skrjabini demonstrated a significant relation in the cluster and an early common ancestor with isolates of the two-humped camel (China). As for the COX1 gene and ITS-2 region, the phylogenetic relationship supported the ribosomal gene results, especially with Habronema muscae or related species such as Habronema majus, Dirofilaria repens, Dipetalonema evansi, Setaria tundra, Cercopithifilaria sp towards the root node. Therefore, considering COX1 gene and ITS-2 region as an ideal tool in determining the phylogenetic history of the sequence maps, but less conservative mode than the ITS2 ribosome gene based to a taxonomic species level.

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“…The ticks were intensively examined under the evaluation of Prof. Dr. Monyer Abdulameir Abd Alfatlawi (specialist in veterinary parasitology), Head of Department of Microbiology, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah City, Iraq. The ticks were visualized under a dissecting microscope and relying on standard information regarding methods and tick morphological features described by Soulsby (14) and Shubber et al (15).…”

Section: Microscopic Examinationmentioning

confidence: 99%

Genotypic analysis of ticks species infesting cattle in Al-Diwaniyah abattoir

Ali¹,

Atiyah²,

Al-Fatlawi³

et al. 2021

IJVS

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Different vectors are considered critical for disease transmission between animals; however, ticks play a significant role in the dissemination of various infectious illnesses of animals and human importance. The current work was carried out to categorize ticks genetically of those isolated from cattle that entered Al-Diwaniyah abattoir. In the present study, 50 tick samples were collected and subjected to microscopic examination and genetic-based methods of polymerase chain reaction and partial gene sequencing, both utilized the mitochondrial cytochrome c oxidase subunit I (COX1) gene as a genotyping element. The findings of the microscopic examination showed that the ticks were from Hyalomma spp. Further analysis, the polymerase chain reaction revealed the genus of Hyalomma of the ticks, but when the PGS was performed, one sample of H. detritum, three samples of H. excavatum, and two samples of H. marginatum were identified. When the phylogenetic analyses were conducted, H. detritum showed close genetic similarity to an isolate from Spain EU827695.1. H. excavatum revealed similarity with isolates from India MK863382.1 and Turkey MT230050.1. In contrast, H. marginatum displayed close identity to an isolate from Iran (MG557555.1). In conclusion, these findings may indicate evolutionary links of the locally identified isolates to different world isolates, probably due to the trade-moving of animals.

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Molecular differentiation of Thysaniezia (Helictometra) giardi and Moniezia species based on 18s rRNA gene in small ruminants

Cited by 5 publications

References 16 publications

Morphological and phylogenetic characterization of Oestrus ovis larvae in sheep: Al-Qadisiyah province, Iraq

Morphological and phylogenetic characterization of Oestrus ovis larvae in sheep: Al-Qadisiyah province, Iraq

COX1 gene and ITS-2 region: A comparative study of molecular diagnosis of Parabronema skrjabini in camels (Camelus dromedaries), Al-Najaf Province, Iraq

Genotypic analysis of ticks species infesting cattle in Al-Diwaniyah abattoir

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