Molecular differentiation of Panax notoginseng grown under different conditions by internal extractive electrospray ionization mass spectrometry and multivariate analysis

Zhu, Xiaoping; Chen, Ze-Yan; Qiu, Zi-Dong; Liu, Mingxing; Xu, Jiaquan; Lai, Chang-Jiang-Sheng; Frankevich, Vladimir; Chingin, Konstantin

doi:10.1016/j.phytochem.2021.113030

Cited by 6 publications

(8 citation statements)

References 41 publications

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“…Dominant mass peaks, including m / z 341, m / z 377, m / z 719, and m / z 1061, were found in the mass spectra obtained from all the ginseng samples. In Figure 2 a, the characteristic mass peak m / z 539 was present, but there was no mass peak m / z 1193 [ 24 ], as shown in Figure 2 b,c. Thus, the chemical fingerprints of the three different ginseng samples were similar to each other, but diversity could also be recognized in some metabolites.…”

Section: Resultsmentioning

confidence: 99%

“…The chloride anions were produced via the electrochemical reduction of chlorinated solvents at the ESI capillary, as reduction processes were inherent to the negative ion ESI process, leading to the formation of chloride adducts [M+Cl] − , which competed with the formation of the deprotonated molecules [M-H] − . The relationship between the two species was a difference of 36 Da [ 24 ], as shown in Figure 4 f.…”

Section: Resultsmentioning

confidence: 99%

“…The addition of ammonium chloride in the methanol solution (0.5 mM ammonium chloride) enhanced the signals of ginsenosides, and formed some of the chlorination peaks of the ginsenosides, as shown in Figure S3 for the MS 2 spectrum of the Rg1/Rf chlorination peak m / z 835 and Re/Rd chlorination peak m / z 981; in the negative ion mode, ginsenosides [M+Cl] − and [M-H] − had a difference of 36 Da, forming m / z 799 and m / z 945, respectively [ 24 ].…”

Section: Resultsmentioning

confidence: 99%

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Metabolite Fingerprinting for Identification of Panax ginseng Metabolites Using Internal Extractive Electrospray Ionization Mass Spectrometry

Yuan

Zhang

et al. 2023

Foods

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Ginseng, a kind of functional food and medicine with high nutritional value, contains various pharmacological metabolites that influence human metabolic functions. Therefore, it is very important to analyze the composition and metabolites of ginseng. However, the analysis of active metabolites in ginseng samples usually involves various experimental steps, such as extraction, chromatographic separation, and characterization, which may be time-consuming and laborious. In this study, an internal extractive electrospray ionization mass spectrometry (iEESI-MS) method was developed to analyze active metabolites in ginseng samples with sequential sampling and no pretreatment. A total of 44 metabolites, with 32 ginsenosides, 6 sugars, and 6 organic acids, were identified in the ginseng samples. The orthogonal partial least-squares discriminant analysis (OPLS-DA) score plot showed a clear separation of ginseng samples from different origins, indicating that metabolic changes occurred under different growing conditions. This study demonstrated that different cultivation conditions of ginseng can be successfully discriminated when using iEESI-MS-based metabolite fingerprints, which provide an alternative solution for the quality identification of plant drugs.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Metabolite Fingerprinting for Identification of Panax ginseng Metabolites Using Internal Extractive Electrospray Ionization Mass Spectrometry

Yuan

Zhang

et al. 2023

Foods

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“…Ambient MS in which analytes are ionized at ambient pressure is often employed ( Xin et al, 2014 ; Massaro et al, 2021 ; Pereira et al, 2021 ), although inductively coupled plasma (ICP) MS for multi-element fingerprinting ( Zhao et al, 2017 ) and matrix-assisted laser desorption/ionization (MALDI) MS ( Lai et al, 2012 ) have also been reported. Ambient ionization techniques applicable to herbal materials and products include desorption electrospray ionization (DESI) ( Talaty et al, 2005 ; Freitas et al, 2019 ), ESI on solid substrates ( Deng and Yang, 2013 ; Hu et al, 2014 ; Wang H. et al, 2014 ; Xin et al, 2014 ; Jun et al, 2016 ), tissue ESI and related techniques ( Chan et al, 2011 ; Liu et al, 2011 ; Hu et al, 2013 ), extractive ESI ( Lee et al, 2021 ; Zhang et al, 2022 ), direct analysis in real-time (DART) ( Kumar et al, 2015 ; Wang et al, 2021a ), and desorption atmospheric pressure chemical ionization (DAPCI) ( Pi et al, 2011 ).…”

Section: Applications Of Fingerprinting Methodsmentioning

confidence: 99%

“…To predict which class or cluster an unknown sample belongs to, prediction models using supervised classification methods can be implemented. For example, Zhang et al recently performed PCA and OPLS-DA on 90 P. notoginseng samples grown under different conditions ( Zhang et al, 2022 ). Results from the PCA and OPLSDA are in agreement in which there were 12–14 MS peaks that mainly contributed to the differentiation.…”

Section: Applications Of Fingerprinting Methodsmentioning

confidence: 99%

Advances in Fingerprint Analysis for Standardization and Quality Control of Herbal Medicines

2022

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Herbal drugs or herbal medicines (HMs) have a long-standing history as natural remedies for preventing and curing diseases. HMs have garnered greater interest during the past decades due to their broad, synergistic actions on the physiological systems and relatively lower incidence of adverse events, compared to synthetic drugs. However, assuring reproducible quality, efficacy, and safety from herbal drugs remains a challenging task. HMs typically consist of many constituents whose presence and quantity may vary among different sources of materials. Fingerprint analysis has emerged as a very useful technique to assess the quality of herbal drug materials and formulations for establishing standardized herbal products. Rather than using a single or two marker(s), fingerprinting techniques take great consideration of the complexity of herbal drugs by evaluating the whole chemical profile and extracting a common pattern to be set as a criterion for assessing the individual material or formulation. In this review, we described and assessed various fingerprinting techniques reported to date, which are applicable to the standardization and quality control of HMs. We also evaluated the application of multivariate data analysis or chemometrics in assisting the analysis of the complex datasets from the determination of HMs. To ensure that these methods yield reliable results, we reviewed the validation status of the methods and provided perspectives on those. Finally, we concluded by highlighting major accomplishments and presenting a gap analysis between the existing techniques and what is needed to continue moving forward.

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Applications of ambient ionization mass spectrometry in 2022: An annual review

Rankin‐Turner

Sears

Heaney

2023

Analytical Science Advances

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The development of ambient ionization mass spectrometry (AIMS) has transformed analytical science, providing the means of performing rapid analysis of samples in their native state, both in and out of the laboratory. The capacity to eliminate sample preparation and pre‐MS separation techniques, leading to true real‐time analysis, has led to AIMS naturally gaining a broad interest across the scientific community. Since the introduction of the first AIMS techniques in the mid‐2000s, the field has exploded with dozens of novel ion sources, an array of intriguing applications, and an evident growing interest across diverse areas of study. As the field continues to surge forward each year, ambient ionization techniques are increasingly becoming commonplace in laboratories around the world. This annual review provides an overview of AIMS techniques and applications throughout 2022, with a specific focus on some of the major fields of research, including forensic science, disease diagnostics, pharmaceuticals and food sciences. New techniques and methods are introduced, demonstrating the unwavering drive of the analytical community to further advance this exciting field and push the boundaries of what analytical chemistry can achieve.

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Molecular differentiation of Panax notoginseng grown under different conditions by internal extractive electrospray ionization mass spectrometry and multivariate analysis

Cited by 6 publications

References 41 publications

Metabolite Fingerprinting for Identification of Panax ginseng Metabolites Using Internal Extractive Electrospray Ionization Mass Spectrometry

Metabolite Fingerprinting for Identification of Panax ginseng Metabolites Using Internal Extractive Electrospray Ionization Mass Spectrometry

Advances in Fingerprint Analysis for Standardization and Quality Control of Herbal Medicines

Applications of ambient ionization mass spectrometry in 2022: An annual review

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