Molecular Diagnostics in Human Papillomavirus-Related Head and Neck Squamous Cell Carcinoma

Wai, Katherine C.; Strohl, Madeleine P.; Zante, Annemieke van; Ha, Patrick K.

doi:10.3390/cells9020500

Cited by 27 publications

(34 citation statements)

References 81 publications

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“…The eventual feasibility of the use of the sensor in biological relevant media was evaluated by establishing a calibration curve of E7 protein in saliva samples spiked with different concentrations of E7. Indeed, one widely accepted detection scenario is based on HPV-16 E7 detection in saliva [34]. Saliva, which is 90.5% composed of water, is in addition composed of a variety of electrolytes, including sodium, potassium, calcium, magnesium, chloride, bicarbonate, and phosphate.…”

Section: Specific Sensing Of Hpv-16 E7mentioning

confidence: 99%

Reduced graphene oxide–based field effect transistors for the detection of E7 protein of human papillomavirus in saliva

et al. 2020

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Several challenging biological sensing concepts have been realized using electrolyte-gated reduced graphene oxide field effect transistors (rGO-FETs). In this work, we demonstrate the interest of rGO-FET for the sensing of human papillomavirus (HPV), one of the most common sexually transmitted viruses and a necessary factor for cervical carcinogenesis. The highly sensitive and selective detection of the HPV-16 E7 protein relies on the attractive semiconducting characteristics of pyrene-modified rGO functionalized with RNA aptamer Sc5-c3. The aptamer-functionalized rGO-FET allows for monitoring the aptamer-HPV-16 E7 protein binding in real time with a detection limit of about 100 pg mL −1 (1.75 nM) for HPV-16 E7 from five blank noise signals (95% confidence level). The feasibility of this method for clinical application in point-of-care technology is evaluated using HPV-16 E7 protein suspended in saliva and demonstrates the successful fabrication of a promising field effect transistor biosensor for HPV diagnosis.

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Section: Specific Sensing Of Hpv-16 E7mentioning

confidence: 99%

Reduced graphene oxide–based field effect transistors for the detection of E7 protein of human papillomavirus in saliva

et al. 2020

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“…The identification of HPV in head and neck SCCs, by in situ hybridization or PCR, especially in tumors of the oropharynx (tonsil, base of tongue and soft palate) can be useful, as those associated with HPV have better therapeutic response, higher survival rates and lower risk of recurrence when compared to HPV-negative patients. 16 , 20 , 21 …”

Section: Complementary Diagnosismentioning

confidence: 99%

“…In the case of oropharyngeal SCCs, p16 protein screening by immunohistochemistry in tumor tissues is considered an excellent marker and an acceptable substitute for HPV screening. 21 …”

Section: Complementary Diagnosismentioning

confidence: 99%

“…Moreover, regarding head and neck carcinomas, in situ hybridization of RNA is capable of detecting E6/E7 mRNA and allows the observation of transcriptional viral activity in tumor cells and seems to be the most specific test for HPV. 21 Mutations in the NRF2, KEAP1 and CUL3 genes, leading to gain of function in the NRF2 pathway and in the Tp53 gene, are more prevalent among HPV-negative head and neck carcinomas when compared to HPV-positive ones. These molecular biomarkers could also be useful in patient stratification for treatment.…”

Section: Complementary Diagnosismentioning

confidence: 99%

“… 20 Recent technologies are being studied aiming to detect HPV genetic material in serum and saliva, as possible non-invasive tools for the monitoring and follow-up of the treatment of patients with oropharyngeal SCC. 21 …”

Section: Complementary Diagnosismentioning

confidence: 99%

See 2 more Smart Citations

Update on human papillomavirus – Part II: complementary diagnosis, treatment and prophylaxis

Araújo

Magalhães

Garcia

et al. 2021

Anais Brasileiros de Dermatologia

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In this nonsystematic review, the complementary diagnosis, treatment, prevention, and control of human papillomavirus are discussed. The histopathology is addressed regarding its indications, main findings and limitations, as a complementary diagnostic method largely used by dermatologists. Electron microscopy is briefly reviewed, along with its contribution to the accumulated knowledge on HPV, as well as the relevance of research in using this technology for future advances in diagnosis and treatment. Molecular information about the virus is continuously increasing, and the practical applications of HPV serology, molecular identification and genotyping are discussed. Vaccines are a valuable tool in primary HPV infection prevention and are now available in many countries; their composition, indications, and adverse effects are revisited. Local and systemic treatment options are reviewed and off-label prescriptions are discussed. Finally, health education focusing on HPV infection as a sexually transmitted infection of worldwide relevance and the many barriers to improve primary and secondary prevention are addressed.

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Preoperative Circulating Tumor HPV DNA and Oropharyngeal Squamous Cell Disease

Lam,

Sangal,

Aggarwal

et al. 2024

JAMA Otolaryngol Head Neck Surg

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ImportanceThe utility of preoperative circulating tumor tissue-modified viral human papillomavirus DNA (TTMV-HPV DNA) levels in predicting human papillomavirus (HPV)–associated oropharyngeal squamous cell carcinoma (HPV+ OPSCC) disease burden is unknown.ObjectiveTo determine if preoperative circulating tumor HPV DNA (ctHPVDNA) is associated with disease burden in patients with HPV+ OPSCC who have undergone transoral robotic surgery (TORS).Design, Setting, and ParticipantsThis cross-sectional study comprised patients with HPV+ OPSCC who underwent primary TORS between September 2021 and April 2023 at one tertiary academic institution. Patients with treatment-naive HPV+ OPSCC (p16-positive) and preoperative ctHPVDNA levels were included, and those who underwent neck mass excision before ctHPVDNA collection were excluded.Main Outcomes and MeasuresThe main outcome was the association of increasing preoperative ctHPVDNA levels with tumor size and lymph node involvement in surgical pathology. The secondary outcome was the association between preoperative ctHPVDNA levels and adverse pathology, which included lymphovascular invasion, perineural invasion, or extranodal extension.ResultsA total of 70 patients were included in the study (65 men [93%]; mean [SD] age, 61 [8] years). Baseline ctHPVDNA levels ranged from 0 fragments/milliliter of plasma (frag/mL) to 49 452 frag/mL (median [IQR], 272 [30-811] frag/mL). Overall, 58 patients (83%) had positive results for ctHPVDNA, 1 (1.4%) had indeterminate results, and 11 (15.6%) had negative results. The sensitivity of detectable ctHPVDNA for identifying patients with pathology-confirmed HPV+ OPSCC was 84%. Twenty-seven patients (39%) had pathologic tumor (pT) staging of pT0 or pT1, 34 (49%) had pT2 staging, and 9 patients (13%) had pT3 or pT4 staging. No clinically meaningful difference between detectable and undetectable preoperative ctHPVDNA cohorts was found for tumor size or adverse pathology. Although the median preoperative ctHPVDNA appeared to be higher in pT2 through pT4 stages and pN1 or pN2 stages, effect sizes were small (pT stage: η2, 0.002 [95% CI, −1.188 to 0.827]; pN stage: η2, 0.043 [95% CI, −0.188 to 2.600]). Median preoperative log(TTMV-HPV DNA) was higher in active smokers (8.79 [95% CI, 3.55-5.76]), compared with never smokers (5.92 [95% CI, −0.97 to 1.81]) and former smokers (4.99 [95% CI, 0.92-6.23]). Regression analysis did not show an association between tumor dimension or metastatic lymph node deposit size and preoperative log(TTMV-HPV DNA). After univariate analysis, no association was found between higher log(TTMV-HPV DNA) levels and adverse pathology.Conclusions and RelevanceIn this cross-sectional study, preoperative ctHPVDNA levels were not associated with disease burden in patients with HPV+ OPSCC who underwent TORS.

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Molecular Diagnostics in Human Papillomavirus-Related Head and Neck Squamous Cell Carcinoma

Cited by 27 publications

References 81 publications

Reduced graphene oxide–based field effect transistors for the detection of E7 protein of human papillomavirus in saliva

Reduced graphene oxide–based field effect transistors for the detection of E7 protein of human papillomavirus in saliva

Update on human papillomavirus – Part II: complementary diagnosis, treatment and prophylaxis

Preoperative Circulating Tumor HPV DNA and Oropharyngeal Squamous Cell Disease

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