Molecular Diagnostic Tools for the Detection and Characterization of Phoma Tracheiphila

Russo, Michael F; Grasso, F. M.; Bella, P.; Licciardello, Grazia; Catara, A.; Catara, Vittoria

doi:10.17660/actahortic.2011.892.24

Cited by 6 publications

(2 citation statements)

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“…No strategies have been developed, however, to control soil inoculum. Infected plant stems and leaves that have fallen to the ground contribute to increased P. tracheiphilus inoculum in the soil, as do the residual roots of uprooted lemon trees [12][13][14][15][16][17]. Leaving pruning material on the ground has shown that soil containing infected wood can act as a source of inoculum for wounded citrus roots for more than four months [12].…”

Section: Introductionmentioning

confidence: 99%

“…Specific trials also indicate that the soil type does not substantially influence its infective capacity, although clay soil exhibits slightly lower infectivity [18]. Quantitative qPCR has revealed that fungal propagule density is higher in the upper soil layers (first 10 cm), decreasing in the summer and increasing in the autumn [17,19]. Several authors have shown a possible competitive role for antagonistic fungi isolated from soil [3].…”

Section: Introductionmentioning

confidence: 99%

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Soil Microbial Communities in Lemon Orchards Affected by Citrus Mal Secco Disease

Mosca,

Dimaria,

Nicotra

et al. 2024

Genes

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Mal secco is a vascular disease of citrus caused by the mitosporic fungus Plenodomus tracheiphilus. Soil containing infected plant material constitutes an inoculum source for root infections. In this study, the soil bacterial and fungal communities of five lemon orchards located in Syracuse Province (Sicily, Italy) affected by mal secco were analyzed. Soil samples were collected under lemon tree canopies and subjected to total genomic DNA extraction. The fungal DNA was detected through qPCR in all orchards, with variable concentrations. Bacterial and fungal communities were profiled using 16S and ITS amplicon-based high-throughput sequencing, respectively. According to our results, the relative abundances of the most represented bacterial phyla (e.g., Proteobacteria, Actinobacteriota, Acidobacteriota) changed across the orchards, while in the fungal community, the phylum Ascomycota was dominant, with Basidiomycota and Mortierellomycota abundances fluctuating. On the whole, β diversity analysis showed significant variation in the composition of the soil microbial communities across the orchards. This result was confirmed by the analysis of the core community (taxa present at ≥ 75% of total samples), where putative beneficial bacteria resulted in significantly enriched fungus-infected soil samples, suggesting complex microbial interactions. Our findings shed light on the composition and diversity of the soil microbiome in lemon orchards with the occurrence of mal secco infections.

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Section: Introductionmentioning

confidence: 99%