Molecular Diagnosis of Toxoplasma gondii in Aborted Women

Ghasemi, Fatemeh; Rasti, Sima; Bandehpour, Mojgan; Kazemi, Bahram; Piroozmand, Ahmad; Mousavi, Gholam Abbas

doi:10.5812/jjm.15925

Cited by 4 publications

(7 citation statements)

References 4 publications

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“…Based on the results of Ghasemi et al (2015a,b), only the T. gondii IgM seropositivity was significantly higher in women with abortion (3.6%) than normal delivery women (0.9%) (P = 0.37%). Also, Ghasemi et al (2015a) found that the molecular prevalences of T.gondii were seven (6.4%) and two (1.8%) among aborted and normal delivery women, respectively. We observed that the rate of T.gondii infection was associated with contact to cat, raw/half-cooked meat consumption and raw vegetables use in both the case and control groups.…”

Section: Discussionmentioning

confidence: 93%

“…Based on the results of Ghasemi et al. (2015a,b), only the T. gondii IgM seropositivity was significantly higher in women with abortion (3.6%) than normal delivery women (0.9%) ( P = 0.37%). Also, Ghasemi et al.…”

Section: Discussionmentioning

confidence: 94%

“…Also, Ghasemi et al. () found that the molecular prevalences of T.gondii were seven (6.4%) and two (1.8%) among aborted and normal delivery women, respectively.…”

Section: Discussionmentioning

confidence: 97%

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ToRCH “co‐infections” are associated with increased risk of abortion in pregnant women

Rasti

Ghasemi

Abdoli

et al. 2016

Congenital Anomalies

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ToRCH infections (toxoplasmosis, rubella, cytomegalovirus and Herpes simplex virus) have long been known to be associated with bad obstetric outcomes. However, little information is available about the impact of ToRCH co-infections on the outcome of pregnancy. Hence, we tested the IgG and IgM antibodies to Toxoplasma gondii, Rubella, Cytomegalovirus and Herpes Simplex Virus among 81 pregnant women with abortion (case group) and 98 pregnant women with normal delivery (control group). In the single-infection model, only CMV-IgM seropositivity was significantly increased in case than control group (25.9% in case and 12.2 % in control, OR = 2.5, P = 0.019). In the co-infection model, 14 patterns were recognized, but two patterns were significantly increased in the case than the control group. Co-infection of T. gondii IgG + CMV IgM was 9.1-fold increased in the case than the control group (8.6% in the case and 1% in control, OR = 9.1; P = 0.024). Also, co-infection of T. gondii IgG + HSV IgG + CMV IgM was 7.7-fold increased in case than the control group (7.4% in case and 1 % in control, OR = 7.7; P = 0.04). Although the OR of other co-infections was higher in the case than the control group, the difference was not statistically significant. These findings indicate that ToRCH co-infections are associated with increased risk of abortion than single infection. Hence, the rates of co-infections should be considered in prenatal screening of ToRCH infections.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 94%

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ToRCH “co‐infections” are associated with increased risk of abortion in pregnant women

Rasti

Ghasemi

Abdoli

et al. 2016

Congenital Anomalies

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“…Signs and symptoms of the disease can be observed in the acute phase. 2,3 Immune deficiency in people with chronic toxoplasmosis can lead to reactivation of the parasite and emergence of dangerous or even fatal conditions, such as encephalitis. 4,5 A low number of CD4+ lymphocytes in HIV/AIDS patients can lead to increased risk of opportunistic infections or reactivation of chronic infections.…”

Section: Introductionmentioning

confidence: 99%

Epidemiologic evaluation of toxoplasmosis and leading risk factors in HIV/AIDS patients in Arak City, Iran

Aghaee¹,

Saki²,

Didehdar³

et al. 2017

AMJ

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“…The mechanisms by which T. gondii induces pathogenesis in the placental and foetal tissues are not completely clear yet . Exploring the cellular and molecular changes that take place in the placental barrier in relation to toxoplasmosis is still an open field, especially with the unique capability T. gondii to hijack host cell molecules and the recently discovered epigenetic manipulation . Peroxisome proliferator‐activated receptors (PPAR) and sex‐determining region Y‐box 2 (SOX‐2) are important transcription factors in human placenta.…”

Section: Introductionmentioning

confidence: 99%

Human placental PPAR‐γ and SOX‐2 expression in serologically proved toxoplasmosis

Saad

El‐Anwar

Lotfy

et al. 2018

Parasite Immunology

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To explore PPAR-γ and SOX-2 transcription factors expression in placenta according to maternal anti-Toxoplasma gondii serological profile during pregnancy and pregnancy outcome. The study included 240 placentas, grouped according to IgM and IgG serostatus and then subgrouped according to pregnancy outcome that varied between miscarriages, premature labour, stillbirth and giving birth to CNS anomaly or apparently healthy neonates. Samples were H&E stained and histopathologically scored blindly. PPAR-γ expression was measured by ELISA, while SOX-2-positive nuclei were stained immunohistochemically to be calculated by ImageJ. The mean pathological score was significantly higher in IgM+ve and IgG rising than IgG-ve and persistent low groups. Former groups showed significantly higher PPAR-γ (mean = 258.63, 227.11). However, PPAR-γ was higher in apparently healthy neonate subgroups. SOX-2 was significantly lower in IgM+ve and IgG rising groups (mean = 12.87, 43.13) and associated with obvious fibrosis. SOX-2 lowest count was in CNS anomaly subgroup. PPAR-γ and SOX-2 changes may give clues of how Toxoplasma induces pathogenesis during vertical transmission. Triggering PPAR-γ expression may be a tool to downregulate the inflammatory response and establish a metabolically permissive cellular environment for Toxoplasma persistence. Low SOX-2 is suspected to disturb placental mesenchymal stem cells pluripotency and neuroectoderm development.

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Molecular Diagnosis of Toxoplasma gondii in Aborted Women

Cited by 4 publications

References 4 publications

ToRCH “co‐infections” are associated with increased risk of abortion in pregnant women

ToRCH “co‐infections” are associated with increased risk of abortion in pregnant women

Epidemiologic evaluation of toxoplasmosis and leading risk factors in HIV/AIDS patients in Arak City, Iran

Human placental PPAR‐γ and SOX‐2 expression in serologically proved toxoplasmosis

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