Molecular diagnosis of onychomycosis

Petinataud, Dimitri; Berger, Sibel; Contet‐Audonneau, N.; Machouart, M.

doi:10.1016/j.mycmed.2014.10.008

Cited by 29 publications

(26 citation statements)

References 45 publications

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“…An initial issue in the molecular diagnostic approach, that must be addressed, is the protocol of DNA extraction . A reliable molecular diagnosis requires the existence of an appropriate DNA extraction protocol.…”

Section: Discussionmentioning

confidence: 99%

“…In onychomycoses, the technical difficulties in the extraction of DNA from nails are probably connected to the tough and durable nature of the specific material, but also to the rigid fungal cell wall as well as the PCR inhibitors associated with clinical specimens. Also, there is a lack of standardised molecular techniques for the detection of fungal DNA, directly from the clinical samples in nail fungal infections …”

Section: Discussionmentioning

confidence: 99%

“…An initial issue in the molecular diagnostic approach, that must be addressed, is the protocol of DNA extraction. 15 A reliable molecular diagnosis requires the existence of an appropriate DNA extraction protocol. In mycology, a known example of how important is the extraction protocol, is the Aspergillus PCR, wherein the extraction of the genetic material is described as more important than the PCR itself.…”

Section: Discussionmentioning

confidence: 99%

“…Also, there is a lack of standardised molecular techniques for the detection of fungal DNA, directly from the clinical samples in nail fungal infections. 15 The aim of this study was the ranking of three existing separate extraction protocols of DNA from nails and the acquisition of information for possible future use in the molecular diagnosis of onychomycoses, mostly in small and medium size laboratories lacking of automated machinery and by the use of relatively ordinary reagents and apparatus.…”

Section: Discussionmentioning

confidence: 99%

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A comparative study of three extraction protocols of DNA from nails: Potential use in the diagnosis of onychomycoses

Kotrotsiou

Chatzimichaloglou

Nasr

et al. 2016

Mycoses

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Molecular techniques can be useful adjuncts to the diagnosis of onychomycoses. However, the nail presents difficulties in the extraction of its DNA. The comparison of three extraction protocols of DNA from nails and their ranking for possible use in the molecular diagnosis of onychomycoses are described. Extraction was performed on weighed nail clippings of equal size from positive (31) or negative (14) samples, according to the culture result. At Prot1, the extraction was performed according to Tahir and Watson, with an additional step implementing silica columns. At Prot2, the methodology proposed by the Statens Serum Institute of Copenhagen was used. At Prot3, DNA was extracted by the use of magnetic separation after homogenisation with glass beading. The evaluation parameters were DNA purity, DNA concentration, total DNA yield/g of tissue, cost and duration. The multiples of the means of medians of the first three parameters, for each protocol, were calculated. Prot3 showed the highest DNA purity. Prot2 presented the highest DNA concentration and DNA yield/g of tissue, while it was the cheapest and shortest. In total, the three protocols were graded as Prot2>Prot1>Prot3. The second method, although had a lower DNA purity, presented the higher DNA concentration and DNA yield, while its duration and cost were also favourable.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

A comparative study of three extraction protocols of DNA from nails: Potential use in the diagnosis of onychomycoses

Kotrotsiou

Chatzimichaloglou

Nasr

et al. 2016

Mycoses

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“…Seu uso ainda é restrito à pesquisas e em trabalho comparativo para métodos diagnósticos em onicomicose teve 92% de sensibilidade e 42% de especificidade. 11 100%)11 pelo sequenciamento do DNA71 dos fungos na amostra. Muitas variações e refinamento da técnica de PCR tem sido propostas para onicomicose[72][73][74] e além da presença ou ausência de fungos na amostra também é possível através de protocolo "real-time PCR"75 estimar a viabilidade dos fungos através da quantidade relativa de transcrito na amostra.…”

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Padrões de dermatoscopia da placa ungueal nas onicomicoses

Bet¹

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Recent advances in the diagnosis of dermatophytosis

et al. 2020

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Dermatophytosis is a disease of global significance caused by pathogenic keratinolytic fungi called dermatophytes in both animals and humans. The recent taxonomy of dermatophytes classifies them into six pathogenic genera, namely Microsporum, Trichophyton, Epidermophyton, Nannizzia, Lophophyton and Arthroderma. It is because of the delayed diagnostic nature and low accuracy of dermatophyte detection by conventional methods that paved the path for the evolution of molecular diagnostic techniques, which provide the accurate and rapid diagnosis of dermatophytosis for an appropriate, timely antifungal therapy that prevents the nonspecific over‐the‐counter self‐medication. This review focuses on the importance of rapid and accurate diagnosis of dermatophytosis, limitations of conventional methods, selection of targets in diagnosis, and factors affecting sensitivity and specificity of various molecular diagnostic technologies in the diagnosis of dermatophytosis. Generally, all the molecular techniques have a significant edge over the conventional methods of culture and microscopy in the dermatophytosis diagnosis. However, in mycology laboratory, the suitability of any molecular diagnostic technique in the diagnosis of dermatophytosis is driven by the requirement of time, economy, complexity, the range of species spectrum detected and the scale of diagnostic output required. Thus, various choices involved in the pursuit of a diagnosis of dermatophytosis are determined by the available conditions and the facilities in the laboratory.

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Molecular diagnosis of onychomycosis

Cited by 29 publications

References 45 publications

A comparative study of three extraction protocols of DNA from nails: Potential use in the diagnosis of onychomycoses

A comparative study of three extraction protocols of DNA from nails: Potential use in the diagnosis of onychomycoses

Padrões de dermatoscopia da placa ungueal nas onicomicoses

Recent advances in the diagnosis of dermatophytosis

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