Molecular diagnosis of Ehrlichia canis infection in dogs with uveitis

Veloso, Jéssica Fontes; Sauer, Leonardo; Oriá, Arianne Pontes; Gomes, Deusdete Conceição; Raposo, Ana; Andrade, Camila Fernanda Oliveira; Oliveira, Thais Nascimento de Andrade; Carlos, Renata Santiago Alberto

doi:10.5433/1679-0359.2018v39n3p1049

Cited by 5 publications

(2 citation statements)

References 13 publications

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“…Ocular lesions are common in both natural and experimental infections of E. canis, with a prevalence that may vary between 15% and 100% of cases (KOMNENOU et al, 2007;ORIÁ et al, 2008). The commonly reported ocular diseases are anterior uveitis, panuveitis, secondary glaucoma, vitreous hemorrhage and/or retinal detachment, chorioretinitis, optic neuritis and signs related with bleeding disorders such as hyphema and conjunctival hemorrhage among others (LEIVA et al, 2005;KOMNENOU et al, 2007;VELOSO et al, 2018). Hemorrhagic eye disorder may occur in 29.4% of patients infected with E. canis (LEIVA et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Relationship between ocular abnormalities and hematologic alterations in patients infected naturally by Ehrlichia canis

et al. 2021

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To determine the association between ocular abnormalities and hematologic findings in Ehrlichia canis-infected dogs, 120 dogs suspected of canine ehrlichiosis were studied. All patients were subjected to rapid serologic diagnostic test for Ehrlichia canis, and the diagnosis was confirmed by quantitative PCR. PCR-positive patients underwent ophthalmologic examination and a hemogram and were grouped into patients with thrombocytopenia and anemia (G1), patients with thrombocytopenia (G2), and patients without hematologic disorders (G3). For ophthalmic evaluation, the patients were grouped into those having mild, severe, both mild and severe, and no ocular alterations. All patients presented with uveitis (100%). Severe ocular disorders predominated in G1 and G2 patients. Dogs with anemia and leukopenia showed severe ocular disorders. In dogs with a greater number of leukocytes and copies/µl of the TRAG gene, the intraocular pressure (IOP) tends to decrease and vice versa (r = −0.23, r = −0.26). In conclusion, uveitis is the earliest ocular alteration of canine ehrlichiosis. The severity of ocular abnormalities in patients with ehrlichiosis is associated with thrombocytopenia and may be aggravated by anemia and leukopenia. The hemogram and IOP are useful tools for predicting eye involvement in Ehrlichia canis-infected patients. In regions where Ehrlichia canis is endemic, the use of the tonometer should be routinely in dog general consultation.

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Section: Introductionmentioning

confidence: 99%

Relationship between ocular abnormalities and hematologic alterations in patients infected naturally by Ehrlichia canis

et al. 2021

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“…Several PCR modalities were designed to improve the sensitivity of E. canis detection in laboratory methods (DOYLE et al, 2005;NAKAGHI et al, 2010). Nested PCR (nPCR) has been used routinely to detect E. canis to increase analytical sensitivity over cPCR (SALLES et al, 2015;VELOSO et al, 2018;AYAN et al, 2020). Notwithstanding, this method has a high contamination risk that may result in unexpected cross-amplifications (DOYLE et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

New sensitive real-time PCR targeting p28 gene for detection of Ehrlichia canis in blood samples from dogs

et al. 2021

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This study aims to describe a new detection method of a quantitative real-time polymerase chain reaction (qPCR) targeting the 28 kDa outer membrane protein gene (p28) as well as to compare this method with a conventional PCR (cPCR), which targets the same gene, in order to evaluate the performance of the technique designed in this study in detecting Ehrlichia canis (E. canis). Optimum oligonucleotides concentrations were reached, and the analytical sensitivity and specificity of the qPCR were performed. A total of 218 dogs’ whole blood samples were conventionally collected for this study. The DNA was extracted from each sample. Subsequently, the samples were tested by an established cPCR and the new qPCR to compare each technique’s performances. This new qPCR method for the molecular detection of E. canis presented a detection limit of ten copies of the fragment and was considered specific for E. canis according to analytical specificity analyses performed in vitro and in silico. The standard curve revealed 100% efficiency and a coefficient of determination (R2) equivalent to 99.8%. Among the samples examined by qPCR, 24.31% were considered positive, significantly greater than those detected by cPCR (15.13%). The qPCR technique reached a higher sensitivity than the cPCR when targeting the p28 gene in detecting E. canis. The qPCR standardized in this study is an efficient method for confirming canine monocytic ehrlichiosis (CME) diagnosis and might provide the parasitemia monitoring during the disease treatment.

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Molecular prevalence and factors associated with Ehrlichia canis infection in dogs from the North Pantanal wetland, Brazil

et al. 2023

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Background and Aim: Canine monocytic ehrlichiosis is a vector-borne disease caused by the obligatory intracellular bacterium Ehrlichia canis, which is distributed across tropical and subtropical regions worldwide. Its prevalence within dog populations is high in municipalities located across the Pantanal biome, but it remains unknown in Barão de Melgaço, Mato Grosso, Brazil. This study aimed to determine the molecular prevalence and factors associated with E. canis infection in dogs domiciled in Barão de Melgaço. Materials and Methods: A cross-sectional study was carried out to investigate the prevalence of E. canis infection in 369 dogs from urban and rural areas in Barão de Melgaço, North Pantanal wetland, Brazil. Initially, the dogs were examined, and, through a questionnaire, the risk factors were investigated. Blood samples were subjected to DNA extraction and PCR was performed to estimate the prevalence of E. canis infection. Results: The molecular prevalence of E. canis infection in dogs was 42.5% and none of the studied variables were significantly associated with polymerase chain reaction (PCR) positivity (p > 0.05). Conclusion: The high molecular prevalence demonstrates an increased transmission of the agent across the city. This also indicates that attention needs to be paid to E. canis infection and control measures should be introduced to prevent its transmission. The demographic and clinical risk factors commonly associated with E. canis infection in this study were not associated with PCR positivity. Keywords: bacterium, DNA, dog, ehrlichiosis, Pantanal.

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Molecular diagnosis of Ehrlichia canis infection in dogs with uveitis

Cited by 5 publications

References 13 publications

Relationship between ocular abnormalities and hematologic alterations in patients infected naturally by Ehrlichia canis

Relationship between ocular abnormalities and hematologic alterations in patients infected naturally by Ehrlichia canis

New sensitive real-time PCR targeting p28 gene for detection of Ehrlichia canis in blood samples from dogs

Molecular prevalence and factors associated with Ehrlichia canis infection in dogs from the North Pantanal wetland, Brazil

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