Molecular Diagnosis of Cause of Anisakiasis in Humans, South Korea

Lim, Hyemi; Jung, Byung Mun; Cho, Jaeeun; Yooyen, Thanapon; Shin, Eun Hee; Chai, Jong Yil

doi:10.3201/eid2102.140798

Cited by 61 publications

(61 citation statements)

References 14 publications

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“…A. pegreffii infects delphinids, ziphiids, physeterids and neobalaenids as final hosts and has been reported from the waters off Italy, Argentina, Brazil, South Africa and New Zealand (Klimpel et al 2008, Mattiucci & Nascetti 2008, Kellermanns 2009), especially in the temperate zones. Recent cases of anisakiasis caused by A. pegreffii in Korea (Lim et al 2015), Italy and Japan (Mattiucci et al 2013, Lim et al 2015 demonstrate that besides A. simplex (s.s.), A. pegreffii can also become human-pathogenic. In terestingly, A. simplex (s.s.), commonly found in the northern hemisphere and also in Japan in the North Pacific, could not be identified within the present study.…”

Section: Distribution Of Anisakis Sppmentioning

confidence: 99%

“…Some worms can survive inside the human gastrointestinal tract. Especially anisakid nematodes of the genus Anisakis (Anisakidae, Ascaridoidea) have been reported to cause anisakiasis, an inflammation of the human gastrointestinal tract, in many regions worldwide (Ishikura & Namiki 1989, Ishikura & Kikuchi 1990, Klimpel & Palm 2011, Ivanovic et al 2015, Lim et al 2015, Sohn et al 2015, Nieuwenhuizen 2016.…”

Section: Introductionmentioning

confidence: 99%

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Anisakis (Nematoda: Ascaridoidea) from Indonesia

Palm¹,

Theisen²,

Damriyasa³

et al. 2017

Dis. Aquat. Org.

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Section: Distribution Of Anisakis Sppmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Anisakis (Nematoda: Ascaridoidea) from Indonesia

Palm¹,

Theisen²,

Damriyasa³

et al. 2017

Dis. Aquat. Org.

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“…Nine species have so far been detected genetically as belonging to the genus Anisakis . Among these, Anisakis simplex (sensu stricto ) and Anisakis pegreffii are shown to play a zoonotic role in humans . Anisakis pegreffii infection can provoke gastric (GA), intestinal (IA) and gastroallergic anisakiasis (GAA) …”

Section: Introductionmentioning

confidence: 99%

Anisakis pegreffii impacts differentiation and function of human dendritic cells

Napoletano

Mattiucci

Colantoni

et al. 2018

Parasite Immunology

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Human dendritic cells (DCs) show remarkable phenotypic changes when matured in the presence of helminth-derived products. These modifications frequently elicited a polarization towards Th2 cells and regulatory T cells thus contributing to immunological tolerance against these pathogens. In this study, the interaction between DCs and larvae of the zoonotic anisakid nematode Anisakis pegreffii was investigated. A. pegreffii larvae were collected from fish hosts, and monocyte-derived DCs were cocultured in the presence of the live larvae (L) or its crude extracts (CE). In both experimental conditions, A. pegreffii impacted DC viability, hampered DC maturation by reducing the expression of molecules involved in antigen presentation and migration (ie HLA-DR, CD86, CD83 and CCR7), increased the phagosomal radical oxygen species (ROS) levels and modulated the phosphorylation of ERK1,2 pathway. These biological changes were accompanied by the impairment of DCs to activate a T-cell-mediated IFNγ. Interestingly, live larvae appeared to differently modulate DC secretion of cytokines and chemokines as compared to CE. These results demonstrate, for the first time, the immunomodulatory role of A. pegreffii on DCs biology and functions. In addition, they suggest a dynamic contribution of DCs to the induction and maintenance of the inflammatory response against A. pegreffii.

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“…In these countries the main source of fresh marine fish for consumption is the Mediterranean Sea, where A. pegreffii is endemic [8]. In other parts of the world, both sibling species have been identified as responsible for the disease, A. simplex (s.s.) being the main agent in Japan and A. pegreffii in Korea [13,29]. The diagnostic disparity between geographically close countries could be explained by the notably different prevalence of parasitation of the two Anisakis species observed in fish between capture zones [13].…”

Section: Discussionmentioning

confidence: 99%

“…In the last two decades, the use of molecular methods as PCR RFLP, DNA sequencing or, more recently DNA microsatellites, has improved the specific identification of larvae of morphologically non-differentiable Anisakis species, such as those of the A. simplex (s.l.) complex [12][13][14]. To date, it has not been established if any other Anisakis species can cause anisakiosis in humans [8].…”

Section: Introductionmentioning

confidence: 99%

First Molecular Diagnosis of Clinical Cases of Gastric Anisakiosis in Spain

Roca-Geronès

Alcover

Godínez-González

et al. 2020

Genes

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Anisakiosis is a fish-borne disease with gastrointestinal and/or allergic symptoms caused by the consumption of raw or undercooked fish parasitized with nematode larvae of the genus Anisakis. In Europe, Anisakis pegreffii has been detected as the causative agent, although the sibling species Anisakis simplex sensu stricto (s.s.) is also known to cause the disease in other parts of the world, and discrepancies exist regarding their respective pathogenic potential. In Spain a high number of cases has been recorded, with marinated anchovies being the main source of infection, although no specific diagnosis has been documented in humans. In this study, we analyzed three cases of anisakiosis in patients from Barcelona (Spain) who had consumed undercooked hake. All patients described epigastric pain and several larval nematodes were removed endoscopically from their stomachs. Larvae were morphologically characterized as third-stage larvae of Anisakis simplex sensu lato (s.l.) and molecularly identified as A. simplex (s.s.) by means of PCR RFLP of the ITS region of the rDNA and sequencing of the elongation factor1 alpha1 (EF1 α-1) nDNA gen. This study represents the first specific identification of Anisakis larvae in clinical cases of anisakiosis reported in Spain. Specific molecular diagnosis is of crucial importance for assessing the health risk of Anisakis sibling species. Hake consumption stands out as a risk factor for anisakiosis, since this fish species can be highly parasitized.

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Molecular Diagnosis of Cause of Anisakiasis in Humans, South Korea

Cited by 61 publications

References 14 publications

Anisakis (Nematoda: Ascaridoidea) from Indonesia

Anisakis (Nematoda: Ascaridoidea) from Indonesia

Anisakis pegreffii impacts differentiation and function of human dendritic cells

First Molecular Diagnosis of Clinical Cases of Gastric Anisakiosis in Spain

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