Molecular detection of virulent  Mannheimia haemolytica  and  Pasteurella multocida  in  lung tissues of pneumonic sheep from semiarid tropics, Rajasthan, India

Singh, Fateh; Sonawane, G. G.; Meena, Rajendra K.

doi:10.3906/vet-1802-73

Cited by 11 publications

(6 citation statements)

References 15 publications

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“…Isolation and identification of M. haemolytica and P. multocida based on the cultural and biochemical characters and by the PCR assays targeting the specific genes have been reported earlier similar to the present study [13,31]. Moreover, the specific identification of P. multocida by 16S rRNA and KMT1 gene and the characterization by capsular typing has a significant impact on the epidemiology and control of pasteurellosis in domestic animals [8,32,33]. It is also proposed that the molecular typing methods provide rapid bacterial identification and have proved to be more specific over the cultural and biochemical tests [13,34].…”

Section: Discussionsupporting

confidence: 77%

Isolation and molecular characterization of Mannheimia haemolytica and Pasteurella multocida associated with pneumonia of goats in Chhattisgarh

Rawat¹,

Gilhare²,

Kushwaha³

et al. 2019

Vet World

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Aim: The purpose of this study was to isolate and characterize the Mannheimia haemolytica and Pasteurella multocida from blood, nasal discharge, and lung tissue of pneumonic goats. Materials and Methods: A total of 14 goats were investigated for pneumonic pasteurellosis. Of 14 goats, nasal swabs and blood samples were collected from 10 clinically diseased animals. Moreover, lung tissue and heart blood samples were collected during necropsy of four goats died with pneumonia. All the samples were processed for the isolation of M. haemolytica and P. multocida in the laboratory. Bacterial isolates were identified by cultural and biochemical characters and 16S rRNA sequence analysis. All the isolates were subjected to susceptibility testing using commonly used antimicrobials. M. haemolytica isolates were characterized by PHSSA gene detection. P. multocida isolates were characterized by KMT1 gene detection and capsule typing. Results: On necropsy of dead goats, the pneumonia was characterized as acute fibrinous bronchopneumonia. Bacterial culture revealed the isolation of M. haemolytica (7) and P. multocida (5) of 10 clinical cases. Moreover, M. haemolytica and P. multocida were coisolated from two of the lung tissues. Furthermore, one of the other two lung tissues showed the isolation of M. haemolytica while the other showed recovery of P. multocida. Bacterial isolates were specifically identified by the 16S rRNA sequence analysis. The isolates showed reduced susceptibility to β-lactams, aminoglycosides, and fluoroquinolones . Moreover, the PHSSA and KMT1 genes were specifically detected among M. haemolytica, and P. multocida isolates, respectively. All P. multocida isolates belonged to serogroup A. Conclusion: The present study reported an occurrence of pneumonic pasteurellosis caused by M. haemolytica and P. multocida in a goat flock.

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Section: Discussionsupporting

confidence: 77%

Isolation and molecular characterization of Mannheimia haemolytica and Pasteurella multocida associated with pneumonia of goats in Chhattisgarh

Rawat¹,

Gilhare²,

Kushwaha³

et al. 2019

Vet World

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“…Of the 213 cultures two (2) nasal swabs were tentatively identi ed as M.haemolytica. This nding is lower than the ndings of [32], [33], [34] whose reports were 2.22% in sheep and goat, 11.2% in sheep and 14.10% and 11.80% in sheep and goats respectively.…”

Section: Discussioncontrasting

confidence: 73%

MALDI TOF Mass Spectrometry and Molecular detection of Mannheimia haemolytica from sheep and goats in Holeta and Sebeta Town, Oromia Special Zone, Ethiopia

Umer,

Bulto,

Bekele

et al. 2023

Preprint

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Background:Mannhemia haemolytica is one of the most important bacteria among causative agent of pneumonic pasteurellosis in small ruminants throughout worldwide and it is also economically devastating pathogen in Ethiopia. Methods: A cross sectional study was carried out from November 2021 to May 2022 with the aim to identify Mannheimia haemolytica from sheep and goat in Sebeta and Holeta town, Oromia special zone, Ethiopia. A total of 235 samples (213 nasal swabs and 22 whole blood) were collected. Sheep and goat with clinical signs suggestive of pneumonic pasteurollosis were purposively sampling. Bacterial identification was conducted using biochemical, Biolog, Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI TOF MS) and Real time PCR detection. Moreover, antimicrobials susceptibility test was also conducted on the identified bacterial isolates using disc diffusion method. Results: The result showed that from a total of 235 samples, only two nasal swab samples were positive for M. haemolytica (0.85%). The two isolates were confirmed by all the tests and similar result was obtained by; biochemical, Biolog, MALDI TOF MS and real time PCR. Up on antimicrobial susceptibility testing, the two isolates were resistant to Streptomycin, Erythromycin and Clindamycin whereas they were susceptible to Tetracycline, Chloramphenicol, Trimethoprim/sulfonamides and Penicillin. Generally, this study revealed that M. haemolytica is among the causative agent of pneumonic pasteurellosis in sheep and goat in the study area. Although, the other remaining bacteria responsible for the disease. Conclusion: The research suggests that a combination of diagnostic methods such as MALDI TOF MS, Biolog, and real-time PCR should be used, as well as for a more in-depth investigation to identify the strain or serotype of M. haemolytica using advanced molecular sequencing and also analysis of the remaining causal agent from various species and locations in countries are significant to address the present vaccination and antibiotic resistance issues.

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“…In the evening, the animals returned back to the shed where they were provided with dry and green fodder and the formulated concentrates. Pneumonia was the leading cause of mortality at the farms [7,8]. Clinically, the sheep and goats exhibited coughing, sneezing, nasal discharge, and labored breathing.…”

Section: Geography Of the Area And Disease Overviewmentioning

confidence: 99%

Antimicrobial resistance and phenotypic and molecular detection of extended-spectrum β-lactamases among extraintestinal Escherichiacoli isolated from pneumonic and septicemic sheep and goats in Rajasthan, India

Singh¹,

Sonawane²,

Kumar³

et al. 2019

Turk J Vet Anim Sci

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The present study was planned to isolate Escherichia coli from pneumonic and septicemic sheep and goats and to determine antimicrobial resistance (AMR) patterns of the isolates and evaluate them for the presence of extended-spectrum β-lactamase (ESBL) using the phenotypic and molecular methods. Pneumonic lung tissues and heart blood samples were collected by performing necropsy on sheep (n = 96) and goats (n = 08). The samples were processed and used for bacterial isolation. E. coli isolates (n = 58) including 53 from 34 sheep and 5 from 3 goats were recovered and identified by the cultural and biochemical characteristics and 16S rRNA sequencing. The isolates were tested to ascertain AMR pattern, plasmid features, and ESBL production. The highest rate of resistance (65.5%) was recorded against amoxicillin, enrofloxacin, norfloxacin, and ofloxacin. Eighteen (31%) out of 58 isolates showed the ESBL phenotype by combination disk assay. There was a variability in the number (1-5) and size (2 to >20 kb) of plasmids among isolates. Polymerase chain reaction (PCR) amplification revealed the presence of bla TEM (70.6%) and bla SHV (1.7%) genes. PCR specificity was confirmed by nucleotide sequencing. The present study indicates that the extraintestinal and multidrug-resistant (MDR) E. coli strains were associated with pneumonia and/or septicemia in sheep and goats. Therefore, thorough surveillance and monitoring of MDR bacteria are urgently needed to implement the infection control strategies and to restore the efficacy of available antibiotics.

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Molecular detection of virulent Mannheimia haemolytica and Pasteurella multocida in lung tissues of pneumonic sheep from semiarid tropics, Rajasthan, India

Cited by 11 publications

References 15 publications

Isolation and molecular characterization of Mannheimia haemolytica and Pasteurella multocida associated with pneumonia of goats in Chhattisgarh

Isolation and molecular characterization of Mannheimia haemolytica and Pasteurella multocida associated with pneumonia of goats in Chhattisgarh

MALDI TOF Mass Spectrometry and Molecular detection of Mannheimia haemolytica from sheep and goats in Holeta and Sebeta Town, Oromia Special Zone, Ethiopia

Antimicrobial resistance and phenotypic and molecular detection of extended-spectrum β-lactamases among extraintestinal Escherichiacoli isolated from pneumonic and septicemic sheep and goats in Rajasthan, India

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