Isolation and molecular characterization of Mannheimia haemolytica and Pasteurella multocida associated with pneumonia of goats in Chhattisgarh

Abstract

Aim: The purpose of this study was to isolate and characterize the Mannheimia haemolytica and Pasteurella multocida from blood, nasal discharge, and lung tissue of pneumonic goats. Materials and Methods: A total of 14 goats were investigated for pneumonic pasteurellosis. Of 14 goats, nasal swabs and blood samples were collected from 10 clinically diseased animals. Moreover, lung tissue and heart blood samples were collected during necropsy of four goats died with pneumonia. All the samples were processed for the isolation of M. haemolytica and P. multocida in the laboratory. Bacterial isolates were identified by cultural and biochemical characters and 16S rRNA sequence analysis. All the isolates were subjected to susceptibility testing using commonly used antimicrobials. M. haemolytica isolates were characterized by PHSSA gene detection. P. multocida isolates were characterized by KMT1 gene detection and capsule typing. Results: On necropsy of dead goats, the pneumonia was characterized as acute fibrinous bronchopneumonia. Bacterial culture revealed the isolation of M. haemolytica (7) and P. multocida (5) of 10 clinical cases. Moreover, M. haemolytica and P. multocida were coisolated from two of the lung tissues. Furthermore, one of the other two lung tissues showed the isolation of M. haemolytica while the other showed recovery of P. multocida. Bacterial isolates were specifically identified by the 16S rRNA sequence analysis. The isolates showed reduced susceptibility to β-lactams, aminoglycosides, and fluoroquinolones . Moreover, the PHSSA and KMT1 genes were specifically detected among M. haemolytica, and P. multocida isolates, respectively. All P. multocida isolates belonged to serogroup A. Conclusion: The present study reported an occurrence of pneumonic pasteurellosis caused by M. haemolytica and P. multocida in a goat flock.

Pathomorphological studies of respiratory mannheimiosis in goats

“…Two animals died without manifestation of any clinical sign. The clinical signs observed in the present study were almost similar to those reported earlier [10] . The manifestation of acute respiratory distress usually appears within 10-14 days in adult ruminants after exposed to stressful conditions.…”

“…The results observed were typical to those exhibited by Mannhemia spp. Involvement of Pasteurella multocida and Mannhemia haemolytica in pneumonic goats has also been reported from Chhattisgarh [10] and Western Maharashtra [18] . Pneumonia in small ruminants is primarily caused by parainfluenza-3 virus and respiratory syncytial virus and mycoplasma infection and is predisposed by variable weather conditions [14] .…”

Exploring the Rumen and Cecum Microbial Community from Fetus to Adulthood in Goat

“…These two dominant bacteria, Mannheimia (18.33%) and Bibersteinia (13.63%), are pathogenic microorganisms. Mannheimia haemolytica and Bibersteinia trehalosi are the main causes of pneumonia in sheep and goats of all ages, and can induce septicemia in lambs or kids [35,36]. The results revealed that kids born in a natural environment without quarantine might easily acquire pathogenic microorganisms.…”

Prevalence and antibiotic susceptibility of Mannheimia haemolytica and Pasteurella multocida isolated from ovine respiratory infection: A study from Karnataka, Southern India

“…Increasing prevalence of respiratory infection in small ruminants even after HS and PPR vaccination was a serious concern. Recently respiratory infection outbreaks due to M. haemolytica and P. multocida have been reported in various states of India [ 22 , 23 ]. In the present study, we investigated the involvement of M. haemolytica and P. multocida with the respiratory infection of sheep.…”