Molecular detection of virulence factors and biofilm formation in Pseudomonas aeruginosa obtained from different clinical specimens in Bandar Abbas

Bahador, Nima; Shoja, Saeed; Faridi, Foroogh; Dozandeh-Mobarrez, Banafsheh; Qeshmi, Fatemeh Izadpanah; Javadpour, Sedigheh; Mokhtary, Sedigheh

doi:10.18502/ijm.v11i1.701

Cited by 20 publications

(28 citation statements)

References 13 publications

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“…In addition, it needs for bacterial dissemination during infection (Yousefi-Avarvand et al, 2015). Our results identified exoS gene most prevalent (70%) within the P. aeruginosa isolates which in agreement with other study and similar to (Bahador et al, 2019, Mingxiang et al, 2018. In Poland, Pobiega revealed a rate for exoS (92.3%) among clinical samples (Amirmozafari et al, 2016).…”

Section: Discussionsupporting

confidence: 93%

“…The current study identified that (98%, 85%) of the isolated samples produced biofilm by TCP and CRP method respectively, this is agreed with (Jabalameli et al, 2012, Hamad, 2018. The biofilm production facilitates to ascent bacterial resistant strains and eases the efficacy of antibiotic therapy (Bahador et al, 2019). ExoS is a major cytotoxin that is required for bacterial colonisation and invasion.…”

Section: Discussionmentioning

confidence: 99%

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Evaluating the prevalence of virulence factor gene and biofilm production in Pseudomonas aeruginosa isolated from different clinical samples.

Hussen

2020

ZJPAS

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Pseudomonas aeruginosa is considered a resourceful pathogen; which has several essential virulence effectors such as exoenzyme, exotoxin and biofilm might help to it is infection. This study aimed to investigate the frequency of exoS gene, the determination of biofilm production and antimicrobial resistance among clinical samples of P. aeruginosa. In our study, 227 specimens of P. aeruginosa collected from different clinical specimens which were attending public hospitals in Erbil city. Antimicrobial resistance of samples identified by Kirby-Bauer disk diffusion method. Through PCR virulence gene exoS was studied. Biofilm production measured by both Congo Red Agar (CRA) and tissue culture plate method (TCP). Among 227 clinical samples, 40 (17.6%) were positive for P. aeruginosa. Imipenem was showed most effective antibiotic 95% against P. aeruginosa. Incidence of exoS gene was 70% within the P. aeruginosa isolates. Moreover, around 75% of clinical samples produced biofilm and approximately 40% of them produced strong biofilm. Our study showed that the incidence of bla exoS gene and biofilm formation, which are common virulence factors in the clinical samples, especially in burn patients, and are a severe problem in the treatment of the patient.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Evaluating the prevalence of virulence factor gene and biofilm production in Pseudomonas aeruginosa isolated from different clinical samples.

Hussen

2020

ZJPAS

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“…We used a quantitative phenotypic test considered to be a gold standard to detect biofilm production by clinical isolates of P. aeruginosa. Moreover, different studies that used the same technique to analyze biofilm production by P. aeruginosa obtained percentages for biofilm production ranging from 73.7 % to 98.6 % in the isolates analyzed 1 , 3 , 6 , 9 , 10 , 24 , that were higher than those described in this study.…”

Section: Discussioncontrasting

confidence: 64%

Phenotypic and genotypic analysis of biofilm production by Pseudomonas aeruginosa isolates from infection and colonization samples

Rodrigues

Lima

Sena

et al. 2020

Rev. Soc. Bras. Med. Trop.

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Introduction: Pseudomonas aeruginosa is an opportunistic pathogen associated with healthcare-related infections, affecting mainly patients with underlying diseases and immunosuppression. This microorganism has several virulence mechanisms that favour its pathogenesis, including the production of biofilm. This study aimed to analyze the phenotypic production of biofilms, the occurrence of quorum sensing (QS) genes, and the clonal profile of clinical isolates of P. aeruginosa from colonized/infected patients in a tertiary hospital in Recife-PE. Methods: We obtained 21 isolates that were classified as infection isolates (II), and 10 colonization isolates (CI). The phenotypic analysis for biofilm production was performed quantitatively. The QS genes were detected by specific PCRs, and the clonal profile was assessed using ERIC-PCR. Results: Of the 31 isolates, 58.1 % (18/31) were biofilm producers, of which 70 % (7/10) were CI and classified as weakly adherent; 52.4 % (11/21) of the II produced biofilms, and were classified as weak (38.1 %, (8/21)), moderate (9.5 %, (2/21)), and strongly adherent (4.8 %, (1/21)). All isolates harbored the QS genes analyzed. In the clonal analysis, 26 distinct genetic profiles were identified, highlighting the presence of a clone in four samples, i.e., one infection isolate, and 3 colonization isolates. Conclusions: The detection of biofilm formation is important in P. aeruginosa in addition to the identification of colonization and infection isolates, especially from complex environments such as ICUs. Further, we define a strategy for monitoring and analyzing P. aeruginosa strains that can potentially cause infections in hospitalized patients.

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“…Pseudomonas aeruginosa , as a non-fermenter gram-negative bacterium can survive under various environmental conditions. It is an opportunistic pathogen that can colonize in healthy individuals and moist sites in the hospitals ( 1 ). This bacterium causes a wide range of infections, particularly in immunocompromised, burn, and cystic fibrosis patients.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Antibiotic Resistance Pattern, Alginate and Biofilm Production in Clinical Isolates of Pseudomonas aeruginosa

Davarzani¹,

Saidi²,

Besharati³

et al. 2021

ijph

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Background: Pseudomonas aeruginosa is one of the most common opportunistic bacteria causing nosocomial infections, which has significant resistance to antimicrobial agents. This bacterium is a biofilm and alginate producer. Biofilm increases the bacterial resistance to antibiotics and the immune system. Therefore, the present study was conducted to investigate the biofilm formation, alginate production and antimicrobial resistance patterns in the clinical isolates of P. aeruginosa. Methods: One hundred isolates of P. aeruginosa were collected during the study period (from Dec 2017 to Jul 2018) from different clinical samples of the patients admitted to Milad and Pars Hospitals at Tehran, Iran. Isolates were identified and confirmed by phenotypic and genotypic methods. Antimicrobial susceptibility was specified by the disk diffusion method. Biofilm formation and alginate production were measured by microtiter plate and carbazole assay, respectively. Results: Sixteen isolates were resistant to all the 12 studied antibiotics. Moreover, 31 isolates were MultidrugResistant (MDR). The highest resistance rate was related to ofloxacin (36 isolates) and the least resistance was related to piperacillin-tazobactam (21 isolates). All the isolates could produce the biofilm and alginate. The number of isolates producing strong, medium and weak biofilms was equal to 34, 52, and 14, respectively. Alginate production was more than 400 μg/ml in 39 isolates, 250-400 μg/ml in 51 isolates and less than 250 μg/ml in 10 isolates. Conclusion: High prevalence of MDR, biofilm formation, and alginate production were observed among the clinical isolates of P. aeruginosa. The results also showed a significant relationship between the amount of alginate production and the level of biofilm formation.

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Molecular detection of virulence factors and biofilm formation in Pseudomonas aeruginosa obtained from different clinical specimens in Bandar Abbas

Cited by 20 publications

References 13 publications

Evaluating the prevalence of virulence factor gene and biofilm production in Pseudomonas aeruginosa isolated from different clinical samples.

Evaluating the prevalence of virulence factor gene and biofilm production in Pseudomonas aeruginosa isolated from different clinical samples.

Phenotypic and genotypic analysis of biofilm production by Pseudomonas aeruginosa isolates from infection and colonization samples

Evaluation of Antibiotic Resistance Pattern, Alginate and Biofilm Production in Clinical Isolates of Pseudomonas aeruginosa

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