Molecular Detection of the Arr-2 Gene in Escherichia coli and Klebsiella pneumoniae Resistant to Rifampicin in Abidjan, Côte D'Ivoire

Victoire, Gadou; Guessennd, Nathalie; Toty, Abalé Anatole; Konan, Fernique; Ouattara, Mohamed Baguy; Dosso, Mireille; Diène, Seydina M.; Djaman, Joseph Allico; Rolain, Jean-Marc

doi:10.9734/mrji/2018/40552

Cited by 3 publications

(3 citation statements)

References 23 publications

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“…E. coli is the one which presents the greatest prevalence of resistance (56.7%) even if the 44% presented by K. pneumoniae are not negligible. The high prevalence of ESBLs in this study, as well as some reported in Côte d' d'Ivoire (Guessennd et al, 2011;Gadou et al. 2018) and in other parts of Africa (Togo et al, 2014;Saravanan et al, 2018;Ouchar et al, 2019), show that the spread of ESBL-producing Enterobacteriaceae in an African context remains a very worrisome phenomenon.…”

Section: Discussionsupporting

confidence: 74%

“…blaTEM, blaSHV and blaCTX-M are the main variants of genes coding for resistance in Enterobacteriaceae with the latest one (Ahmad and Khadija, 2019). In Côte d'Ivoire, recent studies have reported the spread of ESBL-producing strains of E. coli and K. pneumoniae in various infections (Tahou et al, 2017;Gadou et al, 2018) although the setup since 2002 of an observatory of antimicrobial resistance is dedicated to actions contributing to fight against the spread of resistant or multi-resistant bacteria (Ouédraogo et al, 2017). In addition, information on genetic variants and intragenic mutations encoding these resistances are scarce.…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization and in silico analysis of mutations associated with extended-spectrum beta-lactamase resistance in uropathogenic Escherichia coli and Klebisiella pneumoniae in two hospitals, Cte dIvoire

Abe¹,

Koffi²,

Sokouri³

et al. 2021

Int. J. Genet. Mol. Biol.

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Escherichia coli and Klebsiella pneumoniae are pathogens frequently involved in urinary tract infections with high epidemic potential. The increase and spread resistance of these microbes to broad spectrum beta-lactam antibiotics are usually reported and is a real public health concern in Côte d'Ivoire but information on genetic variants and intragenic mutations encoding these resistances are scarce. The aim of this study is to characterize genetic variants and describe the intragenic mutations underlying resistance to broad-spectrum beta-lactam antibiotics in uropathogen E. coli and K. pneumoniae in HKB and CHR hospitals with different epidemiological facies in Côte d'Ivoire. 39 strains comprising 30 of E. coli and 9 strains of K. pneumoniae were isolated from which DNA was extracted, amplified and sequenced. ESBLs genes were detected by polymerase chain reaction in 58.8 % of strain analysis. No significant difference was observed between ESBL from HKB and CHR hospitals although HKB and CHR sites present 50 and 56.8% of ESBL respectively. Nucleotide sequences subjected to BLASTn for sequences similarity and homology revealed diversity of resistance genes with dominance of the gene encoding the extended-spectrum β-lactamase CTX-M-15 and the emergence of a new blaTEM-9 gene in Côte d'Ivoire. The significant co-expression of ESBLs might impact 3 rd generation cephalosporin multiresistance among pathogenic bacteria infecting patient population. Routine antibiogram practice could guide the choice of optimal antibiotic therapy for successful treatment and delay the occurrence of multidrug resistance in enterobacterial infections.

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Section: Discussionsupporting

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Molecular characterization and in silico analysis of mutations associated with extended-spectrum beta-lactamase resistance in uropathogenic Escherichia coli and Klebisiella pneumoniae in two hospitals, Cte dIvoire

Abe¹,

Koffi²,

Sokouri³

et al. 2021

Int. J. Genet. Mol. Biol.

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“…The only rifampicin resistance mechanism harboured by our strains was the one linked to ADP-ribosyltransferase (arr) genes 2 and 3, revealed exclusively in the wastewater samples. The arr-1, arr-2 and arr-3 genes carried by class I integrons have been described in Gram-negative bacilli strains in Europe and Asia [64]. The arr-2 gene which was the most frequently detected in the selected strains, particularly in influent samples, has been reported to be associated with several transposons and integrons in K. pneumoniae strains [65].…”

Section: Discussionmentioning

confidence: 99%

Whole genome sequencing snapshot of multi-drug resistant Klebsiella pneumoniae strains from hospitals and receiving wastewater treatment plants in Southern Romania

Surleac

Barbu

Paraschiv³

et al. 2020

PLoS ONE

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We report on the genomic characterization of 47 multi-drug resistant, carbapenem resistant and ESBL-producing K. pneumoniae isolates from the influent (I) and effluent (E) of three wastewater treatment plants (WWTPs) and from Romanian hospital units which are discharging the wastewater in the sampled WWTPs. The K. pneumoniae whole genome sequences were analyzed for antibiotic resistance genes (ARGs), virulence genes and sequence types (STs) in order to compare their distribution in C, I and E samples. Both clinical and environmental samples harbored prevalent and widely distributed ESBL genes, i.e. bla SHV , bla OXA , bla TEM and bla CTX M . The most prevalent carbapenemase genes were bla NDM-1 , bla OXA-48 and bla KPC-2 . They were found in all types of isolates, while bla OXA-162 , a rare bla OXA-48 variant, was found exclusively in water samples. A higher diversity of carbapenemases genes was seen in wastewater isolates. The aminoglycoside modifying enzymes (AME) genes found in all types of samples were aac(6'), ant(2'') Ia, aph(3'), aaD, aac(3) and aph(6). Quinolone resistance gene qnrS1 and the multi-drug resistance oqxA/B pump gene were found in all samples, while qnrD and qnrB were associated to aquatic isolates. The antiseptics resistance gene qacEdelta1 was found in all samples, while qacE was detected exclusively in the clinical ones. Trimethroprim-sulfamethoxazole (dfrA, sul1 and sul2), tetracyclines (tetA and tetD) and fosfomycin (fosA6, known to be located on a transpozon) resistance genes were found in all samples, while for choramphenicol and macrolides some ARGs were detected in all samples (catA1 and catB3 / mphA), while other (catA2,

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Responses of sediment resistome, virulence factors and potential pathogens to decades of antibiotics pollution in a shrimp aquafarm

Lü

Zhang

et al. 2021

Science of The Total Environment

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Molecular Detection of the Arr-2 Gene in Escherichia coli and Klebsiella pneumoniae Resistant to Rifampicin in Abidjan, Côte D'Ivoire

Abstract: Aims: The aim of this study was to demonstrate the presence of rifampicin resistance arr-2 gene in certain enterobacteria of clinical origin from various biological products.

Cited by 3 publications

References 23 publications

Molecular characterization and in silico analysis of mutations associated with extended-spectrum beta-lactamase resistance in uropathogenic Escherichia coli and Klebisiella pneumoniae in two hospitals, Cte dIvoire

Molecular characterization and in silico analysis of mutations associated with extended-spectrum beta-lactamase resistance in uropathogenic Escherichia coli and Klebisiella pneumoniae in two hospitals, Cte dIvoire

Whole genome sequencing snapshot of multi-drug resistant Klebsiella pneumoniae strains from hospitals and receiving wastewater treatment plants in Southern Romania

Responses of sediment resistome, virulence factors and potential pathogens to decades of antibiotics pollution in a shrimp aquafarm

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