Molecular Detection of the Adult Plant Leaf Rust Resistance Gene<i>Lr34</i>in Romanian Winter Wheat Germplasm

Ciucă, M.; Cristina, Daniel; Turcu, A.G.; Ionescu, V.; Sãulescu, N. N.

doi:10.1556/crc.2014.0040

Cited by 6 publications

(3 citation statements)

References 26 publications

(24 reference statements)

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“…In addition, the co-dominant marker csLV34 was used. A sufficiently high degree of association of this marker with the resistance gene Lr34/Yr18/Sr57/Pm38/Bdv1 has been established [22] and verified [27,28]. This marker detects two alleles: csLV34a with amplified fragments of 229 bp, indicating an absence of wheat resistance to leaf rust, and csLV34b -the 150-bp fragments, indicating the presence of such resistance [29].…”

Section: Resultsmentioning

confidence: 98%

Screening of the Bread Wheat Varieties for the Leaf Rust Resistance Gene Lr34/Yr18/Sr57/Pm38/Bdv1

Radchenko¹,

Sandetska²,

Morgun³

et al. 2022

TOASJ

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Background: The allelic composition of the gene Lr34/Yr18/Sr57/Pm38/Bdv1, which is associated with resistance to leaf rust in varieties of bread wheat (Triticum aestivum L.), has been investigated. Methods: Three DNA markers were used to determine the allelic state of the gene Lr34/Yr18/Sr57/Pm38/Bdv1: the co-dominant molecular genetic markers cssfr5 and csLV34 and the microsatellite marker Xgwm295. Results: Among 32 cultivars evaluated for resistance to leaf rust, 4 were highly resistant, 26 were resistant and 2 were moderately susceptible. Using the co-dominant marker cssfr5 based on the detection of the polymorphic state of one of the exons of the gene Lr34/Yr18/Sr57/Pm38/Bdv1, the Lr34(+) allele, which confers resistance to leaf rust, was found in 25% of the studied varieties. The coincidence between the results obtained with the markers cssfr5 and csLV34 was 84.5%. Conclusion: The data of the conducted molecular genetic analysis were supplemented by observations of the resistance of the studied varieties to leaf rust in the field. The obtained data can be used in breeding programs to develop new varieties and breeding lines with leaf rust resistance.

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Section: Resultsmentioning

confidence: 98%

Screening of the Bread Wheat Varieties for the Leaf Rust Resistance Gene Lr34/Yr18/Sr57/Pm38/Bdv1

Radchenko¹,

Sandetska²,

Morgun³

et al. 2022

TOASJ

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“…In CA and NWA, the large number of locations with high potential risks, particularly in Iran, Azerbaijan, Uzbekistan, southern Russia, Turkey, and Georgia, suggest existence of environmental conditions suitable for both the rust fungi. Stripe rust and stem rust on wheat commonly occur in these countries [ 11 , 13 , 29 , 54 , 62 , 63 ]. Barberry leaves with aecia were observed in Azerbaijan, Georgia, Iran, Tajikistan, Turkey, and Uzbekistan [ 13 , 20 , 24 , 64 , 65 ], indicating at least one of the rust fungi infecting barberry.…”

Section: Discussionmentioning

confidence: 99%

Potential Infection Risks of the Wheat Stripe Rust and Stem Rust Pathogens on Barberry in Asia and Southeastern Europe

Sinha

Chen

2021

Plants

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Barberry (Berberis spp.) is an alternate host for both the stripe rust pathogen, Puccinia striiformis f. sp. tritici (Pst), and the stem rust pathogen, P. graminis f. sp. tritici (Pgt), infecting wheat. Infection risk was assessed to determine whether barberry could be infected by either of the pathogens in Asia and Southeastern Europe, known for recurring epidemics on wheat and the presence of barberry habitats. For assessing infection risk, mechanistic infection models were used to calculate infection indices for both pathogens on barberry following a modeling framework. In East Asia, Bhutan, China, and Nepal were found to have low risks of barberry infection by Pst but high risks by Pgt. In Central Asia, Azerbaijan, Iran, Kazakhstan, southern Russia, and Uzbekistan were identified to have low to high risks of barberry infection for both Pst and Pgt. In Northwest Asia, risk levels of both pathogens in Turkey and the Republic of Georgia were determined to be high to very high. In Southwest Asia, no or low risk was found. In Southeastern Europe, similar high or very high risks for both pathogens were noted for all countries. The potential risks of barberry infection by Pst and/or Pgt should provide guidelines for monitoring barberry infections and could be valuable for developing rust management programs in these regions. The framework used in this study may be useful to predict rust infection risk in other regions.

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“…Wheat ( Triticum aestivum L.), for instance, is a widely grown crop that feeds around 35% of people worldwide (Deng et al ., 2019 ; Grote et al ., 2021 ). It has been intensively bred to accumulate desirable traits for making elite cultivars with high productivity (Baenziger, 2016 ; Bedő and Láng, 2015 ) and resistance to various disease pathogens, including stem rust (Ai et al ., 2015 ; Aman et al ., 2020c ; Ciuca et al ., 2015 ; Dakouri et al ., 2013 ). Two natural alleles, Lr34 (encoding an ABC transporter) and Lr67 (encoding a hexose‐proton symporter), are widely used in breeding programs to provide broad‐spectrum resistance against rust (Krattinger et al ., 2009 ; Milne et al ., 2018 ; Moore et al ., 2015 ; Spielmeyer et al ., 2013a , b ).…”

Section: Introductionmentioning

confidence: 99%

A CRISPR‐based lateral flow assay for plant genotyping and pathogen diagnostics

Sánchez

Ali

Islam

et al. 2022

Plant Biotechnology Journal

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Efficient pathogen diagnostics and genotyping methods enable effective disease management and breeding, improve crop productivity and ensure food security. However, current germplas m selection and pathogen detection techniques are laborious, time-consuming, expensive, and not easy to mass-scale application in the field. Here, we optimized a field-deployable lateral flow assay, Bio-SCAN, as a highly sensitive tool to precisely identify elite germplasm and detect mutations, transgenes, and phytopathogens in less than 1 hour, starting from sample isolation to result output using lateral flow strips. As a proof of concept, we genotyped various wheat germplasms for the Lr34 and Lr67 alleles conferring broad-spectrum resistance to stripe rust, confirmed the presence of synthetically produced herbicide-resistant alleles in the rice genome, and screened for the presence of transgenic elements in the genome of transgenic tobacco and rice plants with 100% specificity. We also successfully applied this new assay to the detection of phytopathogens, including viruses and bacterial pathogens in Nicotiana benthamiana, and two destructive fungal pathogens (Puccinia striiformis f. sp. tritici and Magnaporthe oryzae Triticum) in wheat. Our results illustrate the power of Bio-SCAN in crop breeding, genetic engineering, and pathogen diagnostics to enhance food security. The high sensitivity, simplicity, versatility, and infield deployability make the Bio-SCAN as an attractive molecular diagnostic tool for diverse applications in agriculture.

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Molecular Detection of the Adult Plant Leaf Rust Resistance GeneLr34in Romanian Winter Wheat Germplasm

Cited by 6 publications

References 26 publications

Screening of the Bread Wheat Varieties for the Leaf Rust Resistance Gene Lr34/Yr18/Sr57/Pm38/Bdv1

Screening of the Bread Wheat Varieties for the Leaf Rust Resistance Gene Lr34/Yr18/Sr57/Pm38/Bdv1

Potential Infection Risks of the Wheat Stripe Rust and Stem Rust Pathogens on Barberry in Asia and Southeastern Europe

A CRISPR‐based lateral flow assay for plant genotyping and pathogen diagnostics

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