Molecular detection of six virulence genes in Pseudomonas aeruginosa isolates detected in children with urinary tract infection

Badamchi, Ali; Masoumi, H; Javadinia, Shima; Asgarian, Ramin; Tabatabaee, A

doi:10.1016/j.micpath.2017.03.009

Cited by 32 publications

(30 citation statements)

References 24 publications

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“…It was found that the rates of antibiotic resistance of P. aeruginosa were (27.1%) to aztreonam, (11%) to ciprofloxacin, and (5.8%) to ceftazidime, whereas Badamchi et al (2017) reported that (27.1%) of P. aeruginosa were resistant to ciprofloxacin and (25.9%) to ceftazidime. Several surveys from developed and developing countries confirmed the direct link between the irrational antibiotics use and the emergence of resistant strains.…”

Section: Discussionmentioning

confidence: 99%

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Virulence genes and antibiotic resistance of Pseudomonas aeruginosa isolated from patients in the Northwestern of Morocco

Elmouaden

Laglaoui

En-nanei

et al. 2019

J Infect Dev Ctries

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Introduction: Pseudomonas aeruginosa is an ubiquitous bacterium causes various community-acquired and nosocomial infections. In this investigation, we aimed to screen the antibiotic susceptibility patterns and the prevalence of virulence factor genes in a set of Pseudomonas aeruginosa isolated from nosocomial and community-acquired infections in the Northwestern of Morocco. Methodology: A total of 155 of Pseudomonas aeruginosa strains were collected (January 2015 - December 2016) from nosocomial and community-acquired infections at hospital centers and clinical laboratories in the Northwestern of Morocco. Antimicrobial susceptibility test was performed by the standard disk diffusion method. In addition, PCR assays were used for screening five virulence encoding genes (lasB, algD, plcH, exoA, and exoS). Results: Our results revealed that high level of antimicrobial resistance was detected towards aztreonam (27.1%) followed by meropenem (14.2%). The resistance to imipenem was significantly higher in strains isolated from nosocomial infections (12.7%) than strains isolated from community-acquired infections (1.5%). The results highlighted that lasB (98.7%) and exoS (98.7%) were the most frequent virulence genes. Conclusions: This survey provides data about phenotypic and genotypic properties of Pseudomonas aeruginosa emerged in the Northwestern of Morocco. It could be helpful for the health workers to improve infection control measures and to establish a surveillance system.

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Section: Discussionmentioning

confidence: 99%

“…Several surveys from developed and developing countries confirmed the direct link between the irrational antibiotics use and the emergence of resistant strains. To reduce this problem, it is very important to implement infection control measures such as good hand hygiene and judicious use of antimicrobial agents [22,23].…”

Section: Discussionmentioning

confidence: 99%

Virulence genes and antibiotic resistance of Pseudomonas aeruginosa isolated from patients in the Northwestern of Morocco

Elmouaden

Laglaoui

En-nanei

et al. 2019

J Infect Dev Ctries

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“…11,20 For the rest of the genes, the conditions were as follows: preliminary denaturation at 94°C (5 min), 35 cycles of denaturation at 94°C (60 s), annealing at 48°C (60 s) and extension at 72°C (90 s), with a last extension cycle at 72°C for 10 min. 21 PCR products were run on 1.5% agarose gel and were afterward visualized under UV lamb.…”

Section: Dna Extraction Identification and Virulence Gene Detectionmentioning

confidence: 99%

<p>Virulence Constitution of Multi-Drug-Resistant <em>Pseudomonas aeruginosa</em> in Upper Egypt</p>

Hassuna¹,

Mandour²,

Mohamed³

2020

IDR

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Ventilator-associated pneumonia caused by Pseudomonas aeruginosa (P. aeruginosa) is a major health-care problem. In this study, we explored the epidemiology of virulence determinants among multi-drug-resistant (MDR) clinical P. aeruginosa isolates from hospitalized patients with ventilator-associated pneumonia in intensive care units in Upper Egypt. Patients and Methods: MDR P. aeruginosa isolates were screened for the presence of eight virulence factors and typed by ERIC-PCR. Results: A total of 39 clinical MDR isolates were selected out of 173 isolated P. aeruginosa showing a combination of adhesion and cytotoxicity virulence patterns, with the detection of aprA, exoU, exoS, lasB, algD, toxA in 74.3%, 58.9%, 46.1%, 41.2%, 30.7%, 20.5% of the isolates, respectively. The MDR isolates were grouped into 13 different virulence profiles according to the pattern of virulence gene distribution. exoU genotype was more predominant among the P. aeruginosa isolates with more than 48% of the isolates harboring this gene alone, 7% harboring both exoU and exoS and 43.5% harboring exoS gene. An intermediate degree of diversity was detected by ERIC-PCR typing where the isolates were clustered in 7 major groups, indicating possible cross-infection within the hospital. Conclusion: Our results highlight the increased frequency of virulent P. aeruginosa isolates with a shift to the more virulent cytotoxic exoU genotype. Further hospital infection-control measures are mandatory to control the hospital cross-transmission of these highly virulent isolates. This study could vastly be a help to develop efficient treatment policies against P. aeruginosa induced ventilator-associated pneumonia.

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“…Microbial DNA was isolated by UltraClean Microbial DNA isolation kit (MoBio), following the manufacturer's instructions. Virulence genes (effector protein-encoding genes exoS and exoU, elastase producing lasB, alginate encoding algD, alkaline phosphatase producing aprA and haemolytic phospholipase encoding plcH) were amplified using PCR with reaction parameters and primers described by Ullah et al (2017), Badamchi et al (2017) and Ajayi et al (2003). Results were visualized by agarose gel electrophoresis.…”

Section: Haemolytic Activity and Virulence Genesmentioning

confidence: 99%

Groundwater, soil and compost, as possible sources of virulent and antibiotic-resistant Pseudomonas aeruginosa

Kaszab

Radó

Kriszt

et al. 2019

International Journal of Environmental Health Research

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Pseudomonas aeruginosa is a major public health concern all around the world. In the frame of this work, a set of diverse environmental P. aeruginosa isolates with various antibiotic resistance profiles were examined in a Galleria mellonella virulence model. Motility, serotypes, virulence factors and biofilm-forming ability were also examined. Molecular types were determined by pulsed-field gel electrophoresis (PFGE). Based on our results, the majority of environmental isolates were virulent in the G. mellonella test and twitching showed a positive correlation with mortality. Resistance against several antibiotic agents such as Imipenem correlated with a lower virulence in the applied G. mellonella model. PFGE revealed that five examined environmental isolates were closely related to clinically detected pulsed-field types. Our study demonstrated that industrial wastewater effluents, composts, and hydrocarboncontaminated sites should be considered as hot spots of high-risk clones of P. aeruginosa.

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Molecular detection of six virulence genes in Pseudomonas aeruginosa isolates detected in children with urinary tract infection

Cited by 32 publications

References 24 publications

Virulence genes and antibiotic resistance of Pseudomonas aeruginosa isolated from patients in the Northwestern of Morocco

Virulence genes and antibiotic resistance of Pseudomonas aeruginosa isolated from patients in the Northwestern of Morocco

<p>Virulence Constitution of Multi-Drug-Resistant <em>Pseudomonas aeruginosa</em> in Upper Egypt</p>

Groundwater, soil and compost, as possible sources of virulent and antibiotic-resistant Pseudomonas aeruginosa

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