Virulence genes and antibiotic resistance of Pseudomonas aeruginosa isolated from patients in the Northwestern of Morocco

Abstract

Introduction: Pseudomonas aeruginosa is an ubiquitous bacterium causes various community-acquired and nosocomial infections. In this investigation, we aimed to screen the antibiotic susceptibility patterns and the prevalence of virulence factor genes in a set of Pseudomonas aeruginosa isolated from nosocomial and community-acquired infections in the Northwestern of Morocco.
 Methodology: A total of 155 of Pseudomonas aeruginosa strains were collected (January 2015 - December 2016) from nosocomial and community-acquired infections at hospital centers and clinical laboratories in the Northwestern of Morocco. Antimicrobial susceptibility test was performed by the standard disk diffusion method. In addition, PCR assays were used for screening five virulence encoding genes (lasB, algD, plcH, exoA, and exoS).
 Results: Our results revealed that high level of antimicrobial resistance was detected towards aztreonam (27.1%) followed by meropenem (14.2%). The resistance to imipenem was significantly higher in strains isolated from nosocomial infections (12.7%) than strains isolated from community-acquired infections (1.5%). The results highlighted that lasB (98.7%) and exoS (98.7%) were the most frequent virulence genes.
 Conclusions: This survey provides data about phenotypic and genotypic properties of Pseudomonas aeruginosa emerged in the Northwestern of Morocco. It could be helpful for the health workers to improve infection control measures and to establish a surveillance system.

No Correlation between Biofilm Formation, Virulence Factors, and Antibiotic Resistance in Pseudomonas aeruginosa: Results from a Laboratory-Based In Vitro Study

“…QS in Pseudomonas consist of a three interconnected systems, namely the Las, Rhl, and Pqs systems; these systems facilitate the changes needed for the pathogens’ survival, by the sensing of diffusible signal molecules found in their surroundings, which is a proxy for the population density in the niche [ 96 , 97 , 98 ]. However, many antibiotics also have the ability to affect these QS systems in Pseudomonas , either by directly acting on gene expression or by the degradation of these signal molecules [ 99 ]. The emergence of P. aeruginosa isolates with different biofilm-forming capacities may also inform the genetic heterogeneity within these species, which is an important factor for successful infection in humans [ 9 ].…”

Antimicrobial resistance among GLASS pathogens in Morocco: an epidemiological scoping review

“…Although the findings of this study may seem useful, some limitations must be considered when the interpretation of the results is required. The strict focus on GLASS bacteria might have led to oversight of important pathogens like Helicobacter pylori [ 105 , 106 ], and Pseudomonas aeruginosa [ 107 , 108 ], which are of significate public health concern in AMR. The Validity and generalizability of the findings to the entire country’s regions might be affected by the clinical-based, cross-sectional study design of the published papers, mainly collected from Casablanca and Marrakech cities.…”

Study the Ability of Pseudomonas Aeruginosa Isolated from Different Clinical Cases to Biofilm Formation and Detection of Algd Gene

“…The results of the molecular detection of the algD gene that contribute to the production of the alginate layer in P. aeruginosawere: all isolates possessed algD gene about ratio 21(95.4 %) and one isolate did not possessed it , shown in figure (3), The results of this study were in agreement with (27) and (28) While not in agreement with (29) .…”