Molecular detection of Mycoplasma suis in captive white-lipped peccaries (Tayassu pecari) and wild boars (Sus scrofa) in Brazil

Dias, Guilherme Bim; Amaral, Renan Bressianini do; Gatto, Igor Renan Honorato; Lapera, Ivan Moura; Oliveira, Luís Guilherme de; Hoppe, Estevam Guilherme Lux; Machado, Rosangela Zacarias; André, Marcos Rogério

doi:10.1016/j.cimid.2019.01.013

Cited by 18 publications

(20 citation statements)

References 24 publications

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Section: Accepted Articlementioning

confidence: 99%

“…Three studies failed to detect haemoplasmas in ungulates, all of which sampled captive populations: one study included a very small sample of Barbary sheep (Ammotragus lervia) (Santos et al, 2017), whereas the other two sampled peccaries (Tayassu spp.) (da Costa Dias et al, 2019). However, these studies used M. suis-specific primers, which prevented detecting other haemotropic Mycoplasma species.…”

Section: Artiodactylsmentioning

confidence: 99%

“…Among suids, only wild boars (Sus scrofa) have been investigated. In all cases, M. suis was the causative pathogen (Dias et al, 2019;Hoelzle et al, 2010). However, all studies used M. suis-and/or M. parvum-specific primers, which limit knowing whether wild suids can be infected with other haemoplasmas.…”

Section: Artiodactylsmentioning

confidence: 99%

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Worldwide occurrence of haemoplasmas in wildlife: Insights into the patterns of infection, transmission, pathology and zoonotic potential

Millán

Cataldo

Volokhov

et al. 2020

Transbound Emerg Dis

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Section: Accepted Articlementioning

confidence: 99%

Section: Artiodactylsmentioning

confidence: 99%

See 1 more Smart Citation

Worldwide occurrence of haemoplasmas in wildlife: Insights into the patterns of infection, transmission, pathology and zoonotic potential

Millán

Cataldo

Volokhov

et al. 2020

Transbound Emerg Dis

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“…At the genomic level, there are no reports on the diversity of PH affecting pigs in Brazil. Even though M. suis and M. parvum have been detected in domestic and wild pigs from Brazil (Dias et al., 2019; Gatto et al., 2019; Martins et al., 2019; Toledo et al., 2016), and domestic pigs from China (Fu et al., 2017) and Korea (Seo, Kwon, & Kwak, 2019), little is known about the occurrence and genetic diversity of M. parvum . Therefore, cloning and sequencing are important tools used to access the genetic diversity of fastidious agents, using DNA from field samples (Charlebois, Jacques, & Archambault, 2014).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of emerging hemotropic mycoplasmas in domestic pigs from Brazil

Sonálio

Perles

Gatto

et al. 2020

Transbound Emerg Dis

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Mycoplasma suis and Mycoplasma parvum bind strongly to erythrocytes and may cause clinical hemoplasmosis in swine, affecting several age groups. Mycoplasma spp. infected animals may be asymptomatic carriers and/or show nonspecific clinical signs.In Brazil, information on genetic diversity associated with porcine hemoplasmas (PH) has not been described yet. Therefore, this study has aimed to detect, quantify and characterize the genetic diversity of PH in finishing pigs from technified farms in the state of Goiás, central-western Brazil. Ethylenediaminetetraacetic acid-blood samples from 450 swine belonging to 30 different farms from Goiás state were collected at the slaughterhouse. Quantitative real-time PCR (qPCR) assays were performed for the molecular detection and quantification of PH 16S rRNA gene fragments. Cloning and sequencing of 16S and 23S rRNA amplicons were performed to evaluate the genetic diversity. Moreover, a questionnaire was applied to each farm manager to obtain epidemiological information about the herd. The results on qPCR showed herd occurrence of 68.89% for PH. Quantification values (starting quantity [SQ]) ranged from 8.43 × 10 −1 to 4.69 × 10 6 copies/µl, and 52.71% of the samples presented SQ values equal or lower than 1 × 10 3 copies/µl. Risk factors were not evaluated once all farms had at least one positive animal. However, Spearman's coefficient test revealed that the occurrence of PH was inversely associated with the number of farrows per week, weaned piglets per week, and weight at slaughter. Phylogenetic analysis based on maximum likelihood and Bayesian methods showed that the 16S rRNA and 23S rRNA gene sequences obtained from five samples formed a single cluster closely related to M. parvum. Genotype analysis using DNASP software confirmed seven and four different 16S and 23S rRNA genotypes among the cloned amplicons, indicating that there are several genotypes of M. parvum circulating in individual pigs and among pig farms in central-western Brazil. K E Y W O R D S emerging disease, genotypes, intensive pig farming, Mycoplasma parvum, Mycoplasma suis, porcine hemoplasmas | 1163 SONALIO et AL. | INTRODUC TI ONHemotropic mycoplasmas (HMs) are known for infecting several mammal species, including wild and domestic pigs. Three hemoplasmas have been described in swine, namely Mycoplasma suis (Kinsley, 1932), Mycoplasma parvum (Splitter, 1950) and 'Candidatus M. haemosuis ' (Fu et al., 2017). The above-mentioned HMs induce red blood cells (RBCs) to undergo programmed cell death, also known as eryptosis, characterized by cell shrinkage, membrane blebbing, activation of proteases and phosphatidylserine exposure on the outer membrane leading to recognition by macrophages and, therefore, phagocytosis (Felder

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“…In acute cases, infected pigs showed high fever, hemolytic anemia, and death in clinical trials. M. suis infections have been reported worldwide, including in China, and have resulted in significant economic losses in the swine industry [14][15][16][17]. Some investigations have suggested that humans in close contact with M. suis-infected pigs could also become infected, meaning that M. suis could jeopardize human health [15,[18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Mycoplasma suis Alpha-Enolase Subunit Vaccine Induces an Immune Response in Experimental Animals

et al. 2021

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Recombinant protein technology has emerged as an excellent option for vaccine development. However, prior to our study, the immune induction ability of recombinant Mycoplasma suis alpha-enolase (rMseno) in animals remained unclear. The purpose of this study was to develop a rMseno protein subunit vaccine and to determine its ability to elicit an immunological response. To accomplish this, we cloned the gene into pET-15b, expressed it in BL21 cells, and purified it. Following the establishment of immunity, the immunogenicity and potential for protection of rMseno were evaluated in mice and piglets. The results demonstrate that anti-M. suis serum recognized the pure rMseno protein in both mice and piglets as evidenced by high levels of specific anti-rMseno antibodies, significantly increased levels of IFN-γ and IL-4 cytokines, and significantly increased T lymphocyte proliferation index. Piglets also had significantly increased levels of specific IgG1, IgG2a, CD4+, and CD8+ cells. The rMseno findings demonstrated a robust immunological response in mice and piglets, affording partial clinical protective efficacy in piglets.

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Molecular detection of Mycoplasma suis in captive white-lipped peccaries (Tayassu pecari) and wild boars (Sus scrofa) in Brazil

Cited by 18 publications

References 24 publications

Worldwide occurrence of haemoplasmas in wildlife: Insights into the patterns of infection, transmission, pathology and zoonotic potential

Worldwide occurrence of haemoplasmas in wildlife: Insights into the patterns of infection, transmission, pathology and zoonotic potential

Genetic diversity of emerging hemotropic mycoplasmas in domestic pigs from Brazil

Mycoplasma suis Alpha-Enolase Subunit Vaccine Induces an Immune Response in Experimental Animals

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