Molecular detection of intrauterine microbial colonization in women with endometriosis

Khan, Khaleque Newaz; Fujishita, Akira; Masumoto, Hiroshi; Muto, Hideki; Kitajima, Michio; Masuzaki, Hiroaki; Kitawaki, Jo

doi:10.1016/j.ejogrb.2016.01.040

Cited by 135 publications

(177 citation statements)

References 30 publications

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“…Liu and Lang believes the activity of endometrial cells determines the development of EMS [20]. However, a group of scientists followed the explanation of Khan, which is the bacterial contamination hypothesis due to the discovery of bacterial colonization in the endometrium of EMS patients [12,15,21]. In the current study, many significant different OTUs presented in the flora of ET and PF inferred the specific microbial community existed in EMS.…”

Section: Discussionmentioning

confidence: 64%

Microbiota composition and distribution along the female reproductive tract of women with endometriosis

Wei

Zhang

Tang

et al. 2020

Ann Clin Microbiol Antimicrob

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Endometriosis (EMS) is a multifactorial disease that affects 10%-15% women of reproductive age and is associated with chronic pelvic pain and infertility. The pathogenesis of EMS has not been consistently explained until now. In this study, we involved 36 endometriosis patients and 14 control subjects who performed laparoscopic surgery due to gynecological benign tumor. The samples from lower third of vagina (CL), posterior vaginal fornix (CU), cervical mucus (CV), endometrium (ET) and peritoneal fluid (PF), were collected and sequenced by 16S rRNA amplicon. The continuous change of the microbiota distribution was identified along the reproductive tract. The flora in lower reproductive tract (CL, CU) were dominated by Lactobacillus. Significant difference of the community diversity began showing in the CV of EMS patients and gradually increased upward the reproductive tract. It indicates the microbiota in cervical samples is expected to be an indicator for the risk of EMS. This study also highlights the decreasing of Lactobacillus in vaginal flora and the increasing of signature Operational Taxonomic Units (OTUs) in transaction zone (CV) and upper reproductive tract (ET, PF) of EMS patients, which reflect the alteration of microbial community associated with EMS, participation of specific colonized bacteria in the EMS pathogenesis and relationship between microbiota and development of disease.

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Section: Discussionmentioning

confidence: 64%

Microbiota composition and distribution along the female reproductive tract of women with endometriosis

Wei

Zhang

Tang

et al. 2020

Ann Clin Microbiol Antimicrob

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“…was decreased in the endometrial samples that had been derived from the women with endometriosis and after GnRHa treatment. Most recently, the authors confirmed the bacterial culture‐based findings by the molecular method …”

Section: Intrauterine Microbial Colonization After Treatment With An mentioning

confidence: 62%

“…Most recently, the authors confirmed the bacterial culture-based findings by the molecular method. 61…”

Section: Lipopolysaccharide-induced Expression Of Cell-cell Adhesiomentioning

confidence: 99%

Bacterial contamination hypothesis: a new concept in endometriosis

Khan

Fujishita

Hiraki

et al. 2018

Reprod Medicine & Biology

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114

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BackgroundEndometriosis is a multifactorial disease that mainly affects women of reproductive age. The exact pathogenesis of this disease is still debatable. The role of bacterial endotoxin (lipopolysaccharide, LPS) and Toll‐like receptor 4 (TLR4) in endometriosis were investigated and the possible source of endotoxin in the pelvic environment was examined.MethodsThe limulus amoebocyte lysate test was used to measure the endotoxin levels in the menstrual fluid and peritoneal fluid and their potential role in the growth of endometriosis was investigated. Menstrual blood and endometrial samples were cultured for the presence of microbes. The effect of gonadotrophin‐releasing hormone agonist (GnRHa) treatment on intrauterine microbial colonization (IUMC) and the occurrence of endometritis was investigated.Main findings (Results)Lipopolysaccharide regulates the pro‐inflammatory response in the pelvis and growth of endometriosis via the LPS/TLR4 cascade. The menstrual blood was highly contaminated with Escherichea coli and the endometrial samples were colonized with other microbes. A cross‐talk between inflammation and ovarian steroids or the stress reaction also was observed in the pelvis. Treatment with GnRHa further worsens intrauterine microbial colonization, with the consequent occurrence of endometritis in women with endometriosis.ConclusionFor the first time, a new concept called the “bacterial contamination hypothesis” is proposed in endometriosis. This study's findings of IUMC in women with endometriosis could hold new therapeutic potential in addition to the conventional estrogen‐suppressing agent.

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“…Additionally, several studies revealed a positive association between the presence of pathogenic bacteria in the uterine cavity and the onset of the disease. The increased isolation of Actinomyces , Corynebacterium , Enterococcus , E. coli , Fusobacterium , Gardnerella , Prevotella , Propionibacterium , Staphylococcus , and Streptococcus from endometrial samples and menstrual blood from patients diagnosed with endometriosis has established the evidence for this association . Further, the reproductive outcomes of patients with endometriosis are significantly improved after antibiotic therapy, underscoring the impact of pathogens in the disease .…”

Section: Role Of Bacteria In Women's Healthmentioning

confidence: 96%

Deciphering the effect of reproductive tract microbiota on human reproduction

Moreno

Simón

2018

Reprod Medicine & Biology

108

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BackgroundThe female reproductive tract contains an active microbiome comprising mainly bacteria from the Lactobacillus genus, which is associated with a healthy microbiome state. However, spatio‐temporal fluctuations of this microbiome that occur in response to internal and external factors may impact the physiology of the reproductive tract organs and even lead to pathological states.MethodsCurrent literature covering the reproductive tract microbiome is summarized and contextualized in this review.Main findingsThis review presents the current knowledge about the bacterial composition of the lower and upper reproductive tract as well as the impact of the microbiota on women's health and reproduction. We place special focus on the impact of the endometrial microbiome in infertility and assisted reproductive technologies.ConclusionThe assessment of the reproductive tract microbiome adds a new microbiological perspective to human reproduction, pregnancy, and onset of new life, highlighting the importance of considering the evaluation of microbial communities to improve personalized care in reproductive medicine and women's health.

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Molecular detection of intrauterine microbial colonization in women with endometriosis

Cited by 135 publications

References 30 publications

Microbiota composition and distribution along the female reproductive tract of women with endometriosis

Microbiota composition and distribution along the female reproductive tract of women with endometriosis

Bacterial contamination hypothesis: a new concept in endometriosis

Deciphering the effect of reproductive tract microbiota on human reproduction

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