Molecular detection of human papillomavirus-16 among Sudanese patients diagnosed with squamous cell carcinoma and salivary gland carcinoma

Mohamed, Fatima; Aldayem, Leena N; Hemaida, Maisa A; Siddig, Omayma; Osman, Zeinab H.; Shafig, Irene R.; Salih, M. A. M.; Muneer, Mohamed S.; Hassan, Rowa; Ahmed, Eiman Siddig; Hassan, Lamis Ahmed; Bakheet, Osama El Hadi; Edris, Ali Mahmoud Mohammed; Ahmed, Ayman; Mohamed, Nouh Saad; Siddig, Emmanuel Edwar

doi:10.1186/s13104-021-05471-5

Cited by 11 publications

(20 citation statements)

References 22 publications

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“…This might reflect the fact that the cyclin D1 is rarely amplified in HPV-infected tumors [51,52]. Although we do not have information on HPV infection on the studied samples, a previous study on Sudanese patients diagnosed with upper aerodigestive tract cancers showed 26 (17.3%) out of 150 patients were positive for HPV [53].…”

Section: Discussionmentioning

confidence: 81%

Expression of Cyclin D1 in Oral Squamous Cell Carcinoma

et al. 2021

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Background: Cyclin D1 expression regulates normal cell cycle. Its deregulation or overexpression may cause disruption in the normal cell cycle control and lead to cancer progression. In this study, we aimed to study the expression of cyclin D1 in oral squamous cell carcinoma (OSCC) and find its association with the different grades of oral tumors, if any. Methods: This cross-sectional study included 40 formalin-fixed paraffin-embedded tissue blocks specimens of OSCC with variable grades. The expression of cyclin D1 was evaluated through immunohistochemical (IHC) staining. Results: There were 9 female and 31 male samples, with a male-to-female ratio of 3.4:1. The age ranged between 25 and 90 years with an average age of 65.5 years. Twenty-five (62.5%) samples were diagnosed as well-differentiated squamous cell carcinoma (WDSCC) and fifteen (37.5%) as poorly differentiated squamous cell carcinoma (PDSCC). No cases of moderately differentiated squamous carcinoma were included in the study. The expression of cyclin D1 was detected in the cases of WDSCC and a lesser expression was seen in the PDSCC with a P-value of 0.0003, OR 1581 and 95% CI (29.8239 to 83810.7113). Conclusion: Cyclin D1 is expressed in OSCC and stronger expression was detected in WDSCC.

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Section: Discussionmentioning

confidence: 81%

Expression of Cyclin D1 in Oral Squamous Cell Carcinoma

et al. 2021

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“…The Human Papillomavirus Genotyping Kit for 23 types (Yaneng Bioscience co., Shen Zhen, China) was used to perform PCR to amplify the conserved region of HPV gene in conjunction with reverse dot blot (RDB) analysis to identify the HPV subtypes. The membrane strips with fixed type-specific probes can detect infections from multiple HPV subtypes including 23 types (HPV 6,11,16,18,31,33,35,39,42,43,45,51,52,53,56,58,59,66,68,73,81,82,and 83). This method had a sensitivity of 104 copies/mL and a specificity of 99%; β-actin was used as an internal positive control (primer sequence of HPV18 E6E7 used in Table S2).…”

Section: Methodsmentioning

confidence: 99%

Infection with Human Papillomavirus 18 Promotes Alkylating Agent-Induced Malignant Transformation in a Human Esophageal Cell Line

Zhang

Pan

et al. 2021

Chem. Res. Toxicol.

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The relationship between human papillomavirus (HPV) and esophageal cancer (EC) has been controversial, which may be caused by the difference in geographic regions of sample origin. Thus, we conducted a case-control study to find that HPV increased the risk of esophageal cancer, and the HPV18 detection rate is the highest (24.2%) among patients with EC, suggesting that HPV18 could be the most risk subtype of HPV infected. We then identified high-risk HPV18 and N-methyl-N′-nitro-N-nitroso-guanidine (MNNG) to establish a model on the viral etiology cooperating with environmental carcinogens. Het-1A cells containing HPV18 were continuously exposed to MNNG or not; then the morphological phenotype and function assays were performed in 25th passage cells. MNNG promoted the proliferation and invasion abilities and inhibited apoptosis both in Het-1A-HPV18 and control group. However, the Het-1A-HPV18 had a stronger change in phenotypic features and formed more transformed foci in soft agar. Further, Western blot found p53 and p21 were down-regulated, and expression of c-Myc, MMP-2, and MMP-9 and Bcl-2/Bax ratio were up-regulated. Our results revealed that MNNG was easier to induce malignant transformation of Het-1A cells transfected with HPV18. It is good evidence for the close relationship between HPV and the etiology of EC, providing foundation for further study in molecular mechanism and specific intervention targets.

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Section: Introductionmentioning

confidence: 99%

“…18 Patients were seronegative for major viruses including HIV, Hepatitis E virus (HEV), human papillomavirus (HPV), and major human arboviruses such as chikungunya (CHIK), Crimean-Congo hemorrhagic fever (CCHF), dengue (DEN), Rift Valley fever (RVF), West Nile (WNV), and Zika (ZIKV) viruses. [18][19][20][21][22][23][24] Cases were presented at the outpatient clinic at the Royal Care hospital in Khartoum. Following the presentation of the first case an ethical approval was obtained from the research ethics committee (Institutional Review Board).…”

Section: Introductionmentioning

confidence: 99%