Molecular detection of fimH& mrkDgenes of strong biofilm producers & MDR Klebsiella pneumoniae

Abstract

Klebsiella pneumoniae is an adaptable pathogen that forms biofilms on a variety of surfaces. This study's objective was to identify the presence of fimbrial genes (types 1 and 3) in K. pneumoniae strains isolated from various clinical sources based on their antibiotic resistance and ability to form biofilms. According to identification utilizing the vitek 2 technology and confirmation by molecular identification targeting the 16S rRNA gene with a particular primer, forty isolates were identified from clinical specimens. The vitek 2 compact system was utilized to evaluate the antibiotic susceptibility of all the isolates. The findings revealed a range of resistance percentages, including 52.5% for Penicillin, 40.5% for Trimethoprim/Sulfamethoxazole, 34.5% for Cephalosporins, 6.25 % for Fluoroquinolones, and 2.5% for each of Carbapenem, Aminoglycoside, Tetracycline, and Nitrofurantoin. The 96-well microtiter plate technique was utilized to generate biofilms. The results demonstrated that all 40 Klebsiella pneumoniae isolates (100%) produced potent biofilms. In order to identify the genes involved in biofilm formation (fimh & mrkd) and the genes responsible for adhesin in type 1& type 3 fimbriae using traditional PCR method, eleven isolates were chosen for molecular analysis that are powerful biofilm makers and MDR.

Downregulation of the Gene Expression of Efflux Pump Gene (acrA) and its Regulator (ramA) in Klebsiella pneumoniae by Quaternary Ammonium Compounds

“…These results agreed with a local study in Baghdad city done by Ali et al, 15 in which it was observed that out of 39 K. pneumoniae isolates, 1 (2.56%) was a moderate biofilm producer, 21 (53.85%) were weak biofilm producers, and 17 (43.59%) were non-producers. On the contrary, a local study by Jabar et al 16 found that all K. pneumoniae isolates were strong biofilm producers, apart from their origin. Also, the present findings were in disagreement with the study by Makhrmash et al 3 as they revealed that among 56 isolates of K. pneumoniae, 10 (18%) formed weak biofilm, 14 (25%) formed moderate biofilm, and 32 (57%) formed strong biofilm.…”