Molecular Detection of Cytauxzoon spp. in Asymptomatic Brazilian Wild Captive Felids

André, Marcos Rogério; Adania, Cristina Harumi; Machado, Rosangela Zacarias; Allegretti, Silmara Marques; Felippe, Paulo Anselmo Nunes; Silva, Ketty F.; Nakaghi, Andrea Cristina Higa; Dagnone, Ana Silvia

doi:10.7589/0090-3558-45.1.234

Cited by 54 publications

(52 citation statements)

References 19 publications

(31 reference statements)

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“…Cytauxzoon sp. (ANDRÉ et al, 2009) and Hepatozoon sp. (ANDRÉ et al, 2010) DNA obtained from naturally infected wild felids were also used as positive controls.…”

Section: Methodsmentioning

confidence: 99%

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Molecular detection of feline arthropod-borne pathogens in cats in Cuiabá, state of Mato Grosso, central-western region of Brazil

Miceli

Gavioli

Gonçalves

et al. 2013

Rev. Bras. Parasitol. Vet.

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Hemotrophic mycoplasmas (hemoplasmas), Bartonella sp., Hepatozoon sp. and Cytauxzoon felis are prominent pathogens that circulate between cats and invertebrate hosts. The present study aimed to detect the presence of DNA from hemoplasmas, Bartonella sp., Hepatozoon sp. and Cytauxzoon felis, and then confirm it by means of sequencing, in blood samples from cats in Cuiabá, MT, Brazil. From February 2009 to February 2011, blood samples with added EDTA were collected from 163 cats that were being housed in four different animal shelters in the city of Cuiabá, state of Mato Grosso, Brazil and from 15 cats that were admitted to the veterinary hospital of the Federal University of Mato Grosso (UFMT). Out of the 178 cats sampled, 15 (8.4%) were positive for hemoplasmas: four (2.2%) for Mycoplasma haemofelis, 12 (6.7%) for 'Candidatus M. haemominutum' and one (0.5%) for 'Candidatus M. turicensis'. One cat (0.5%), a patient that was attended at the veterinary hospital, was coinfected with M. haemofelis, 'Candidatus M. haemominutum' and 'Candidatus M. turicensis', based on sequencing confirmation. Four cats were positive for Bartonella spp.: three (1.7%) for B. henselae and one (0.5%) for B. clarridgeiae. None of the animals showed Cytauxzoon sp. or Hepatozoon sp. DNA in their blood samples. This study showed that cats housed in animal shelters in the city of Cuiabá, state of Mato Grosso, are exposed to hemoplasmas and Bartonella species.

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“…Cytauxzoon sp. (ANDRÉ et al, 2009) and Hepatozoon sp. (ANDRÉ et al, 2010) DNA obtained from naturally infected wild felids were also used as positive controls.…”

Section: Methodsmentioning

confidence: 99%

“…Although piroplasms similar to Cytauxzoon sp. have been found in cat blood smears in the state of Rio de Janeiro (MENDES-DE-ALMEIDA et al, 2007), molecular confirmation of occurrences of this parasite in Brazil has only been done in wild felids ( ANDRÉ et al, 2009;FILONI et al, 2012). On the other hand, Hepatozoon sp.…”

Section: Mycoplasma Haemofelismentioning

confidence: 99%

Molecular detection of feline arthropod-borne pathogens in cats in Cuiabá, state of Mato Grosso, central-western region of Brazil

Miceli

Gavioli

Gonçalves

et al. 2013

Rev. Bras. Parasitol. Vet.

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“…No presente relato, nenhuma manifestação clínica compatível com a cytauxzoonose foi observada, corroborando com os achados de Soares et al (2004) que detectaram o agente em uma onça parda, e André et al (2009) que identificaram material genético do protozoário em seis jaguatiricas (Leopardus pardalis), duas onças pardas e uma onça pintada (Panthera onca). Em contrapartida, doença fatal em leões (Panthera leo), tigre de bengala e tigre branco (Panthera tigres) foi descrita (Garner et al, 1996, Jakob & Wesemeier, 1996, Peixoto et al, 2007 A presença desse patógeno, na ausência de sinais clínicos, pode estar associada à eficácia do sistema imune, no entanto, a hipótese de uma estirpe menos patogênica envolvida na infecção ou, a possibilidade de que o Puma concolor atue, assim como o Lynx rufus, como hospedeiro reservatório natural da doença deve ser investigada (Meinkoth et al, 2000, Soares et al, 2004.…”

Section: Discussionunclassified

Infección natural por Cytauxzoon felis en onza parda (Puma concolor) de vida libre proveniente de la región suroeste de Mato Grosso do Sul, Brasil

Antunes

Silveira

Oliveira

et al. 2017

Pubvet

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RESUMO.Vários fatores como desmatamento e expansão da agricultura e pecuária tem promovido o contato próximo entre animais selvagens e domésticos, levando ao compartilhamento de patógenos, dentre eles o Cytauxzoon felis. O objetivo com este trabalho é relatar um caso de cytauxzoonose em Puma concolor de vida livre proveniente da região sudoeste de Mato Grosso do Sul, Brasil. O diagnóstico foi obtido por meio de microscopia de sangue periférico e reação em cadeia da polimerase, que neste relato, caracterizou-se como achado acidental, uma vez que manifestações clínicas associadas à doença não foram observadas. A detecção de Cytauxzoon felis alerta para a necessidade de estabelecer a importância epidemiológica dos animais selvagens e o risco que representam para as populações domésticas e selvagens de cativeiro em relação aos patógenos transmitidos por carrapatos. Palavras chave: cytauxzoonose, diagnóstico, piroplasmídeos, felino selvagem Natural infection by Cytauxzoon felis in brown ounce (Puma concolor) of free life from the southwest region of Mato Grosso do Sul, BrazilABSTRACT. Several factors such as deforestation and expansion of agriculture and livestock has promoted the close contact between wild and domestic animals, leading to the sharing of pathogens, including the Cytauxzoon felis. The aim of this study is to report a case of cytauxzoonose in a free-living Puma concolor from the southwest region of Mato Grosso do Sul, Brazil. The diagnosis was obtained through microscopy of peripheral blood smear and polymerase chain reaction, that in this report, was characterized as accidental finding, since clinical manifestations associated to disease were not observed. The

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“…has also been identified in felids in other parts of the world, as well. C. felis infection has been reported in ocelots, 1 pumas, 1 jaguars, 1 and captive-reared lions 23 in Brazil, in addition to the identification of piroplasms morphologically indistinguishable from Cytauxzoon spp. in domestic cats.…”

Section: Introductionmentioning

confidence: 99%

Genetic Variability of Archived Cytauxzoon Felis Histologic Specimens from Domestic Cats in Georgia, 1995–2007

et al. 2009

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Abstract. Cytauxzoon felis infection in domestic cats has historically been nearly 100% fatal. However, increasing reports of domestic cats that survive cytauxzoonosis and reports of asymptomatic cats with C. felis infections suggest the existence of different parasite strains that vary in pathogenicity. The objective of the current study was to obtain epidemiologic information about cytauxzoonosis through genotypic characterization of archived histologic specimens from domestic cats with C. felis infections that were diagnosed in Georgia between 1995 and 2007. Such retrospective data on genetic variability will provide an historic context for current studies of C. felis genotype frequencies. Cytauxzoon felis DNA was obtained from formalin-fixed, paraffin-embedded tissues from infected cats diagnosed with cytauxzoonosis at necropsy. Genetic characterization of C. felis was performed using sequence analysis of the polymerase chain reactionamplified ribosomal internal transcribed spacer regions 1 and 2 (ITS1, ITS2). Eleven different combined ITS1 and ITS2 sequences were identified, the majority of which were identical to those previously reported in fatally infected cats from Georgia. The findings of the current study document the existence of genetically distinct C. felis populations in historical samples and, together with data from contemporary samples, demonstrate a diverse population structure for C. felis.

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Molecular Detection of Cytauxzoon spp. in Asymptomatic Brazilian Wild Captive Felids

Cited by 54 publications

References 19 publications

Molecular detection of feline arthropod-borne pathogens in cats in Cuiabá, state of Mato Grosso, central-western region of Brazil

Molecular detection of feline arthropod-borne pathogens in cats in Cuiabá, state of Mato Grosso, central-western region of Brazil

Infección natural por Cytauxzoon felis en onza parda (Puma concolor) de vida libre proveniente de la región suroeste de Mato Grosso do Sul, Brasil

Genetic Variability of Archived Cytauxzoon Felis Histologic Specimens from Domestic Cats in Georgia, 1995–2007

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