Molecular Detection of Cholera Infection during the Outbreak in Thi-Qar Province/Iraq in 2015-2016

Hanan, Zaman K.

doi:10.1088/1742-6596/1279/1/012068

Cited by 2 publications

(2 citation statements)

References 22 publications

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“…Salmonella genomic DNA was extracted using the Genaid Kit, according to the manufacturer's instructions (Geneaid Biotech, Taiwan), a nanodrop was used to measure the concentration and purity of the DNA, to ensure the presence of the genetic material, it was transferred to an agarose gel at a concentration of 1% at a voltage of 70 volts and 85 mA for 20 minutes, the isolated DNA was used as a template for PCR [9].…”

Section: Genomic Dna Extractionmentioning

confidence: 99%

Phylogenetic Analysis of Biofilm Association Protein (BapA) Amplicons in Salmonella spp. Isolated from Broiler (Ross 308) in Thi-Qar Province/Iraq

Gharkan,

Hanan,

Abdulkareem

2023

IOP Conf. Ser.: Earth Environ. Sci.

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This study was conducted to isolate Salmonella bacteria from broiler (Ross 308). A total of 184 fecal samples were collected from birds, showing symptoms of salmonella infection, from two different fields in Thi Qar Governorate in Al-Islah and Al-Fadhlia Districts. 30 isolates infected with Salmonella were diagnosed, by biochemical tests, API 20 E test and molecularly INVA gene. In this study, the BapA gene responsible for biofilm production was studied. Ensure its presence in isolates of broiler feces, the target piece has been amplified, to determine the effect of variations in the BapA gene on the weights and performance of the study birds, and knowing the extent to which the gene is related to the production of biofilms. The result of the nitrogen base sequencing was to obtain nine genetic mutations in the target segment of the gene, 7 of these mutations were silent mutations, it has no effect on study birds and two mutations are of the type of missense mutation, their effect was to change the amino acid code, where the first boom was in the site 130, changed the nitrogenous base from A to C, changed the amino acid from isoleucine to arginine, and change the code from ATA > CTA, led to a change in the genotype with the mutation, the birds with the CC mutant genotype, suffers from a lack of weight and weak immunity, as for the second boom in the site 571, it changed the nitrogenous base from T to C. The amino acid changes from valine to alanine, change the code from GTG > GCG, thus, mutations led to a change in genotypes, the birds with the mutant genotype were also CC, their weights were somewhat similar to the weights of the uninjured birds, the bacteria were weak or non-producing biofilms.

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Section: Genomic Dna Extractionmentioning

confidence: 99%

Phylogenetic Analysis of Biofilm Association Protein (BapA) Amplicons in Salmonella spp. Isolated from Broiler (Ross 308) in Thi-Qar Province/Iraq

Gharkan,

Hanan,

Abdulkareem

2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…from various sources such as humans, soil, chickens, and wastewater [ 49 ]. Iraq is the only country in the Arabian Peninsula that reported the multidrug-resistant Vibrio cholerae , making it unique to this region [ 316 ].…”

Section: Logical Relation Between Sets Of Multidrug-resistant (Mdr) Microbesmentioning

confidence: 99%

Emerging Status of Multidrug-Resistant Bacteria and Fungi in the Arabian Peninsula

Borgio

Rasdan

Sonbol

et al. 2021

Biology

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We aimed to identify the prevalence and emerging status of multidrug-resistant bacteria and fungi and their associated mortality in nine countries in the Arabian Peninsula. Original research articles and case studies regarding multidrug-resistant bacteria and fungi in the Arabian Peninsula, published during the last 10 years, were retrieved from PubMed and Scopus. A total of 382 studies were included as per the inclusion and exclusion criteria, as well as the PRISMA guidelines, from a thorough screening of 1705 articles, in order to analyse the emerging status and mortality. The emerging nature of >120 multidrug-resistant (MDR) bacteria and fungi in the Arabian Peninsula is a serious concern that requires continuous monitoring and immediate preventive measures. More than 50% (n = 453) of multidrug-resistant, microbe-associated mortality (n = 871) in the Arabian Peninsula was due to MDR Acinetobacter baumannii, Mycobacterium tuberculosis and Staphylococcus aureus infection. Overall, a 16.51% mortality was reported among MDR-infected patients in the Arabian Peninsula from the 382 articles of this registered systematic review. MDR A. baumannii (5600 isolates) prevailed in all the nine countries of the Arabian Peninsula and was one of the fastest emerging MDR bacteria with the highest mortality (n = 210). A total of 13,087 Mycobacterium tuberculosis isolates were reported in the region. Candida auris (580 strains) is the most prevalent among the MDR fungal pathogen in the Arabian Peninsula, having caused 54 mortalities. Active surveillance, constant monitoring, the development of a candidate vaccine, an early diagnosis of MDR infection, the elimination of multidrug resistance modulators and uninterrupted preventive measures with enhanced data sharing are mandatory to control MDR infection and associated diseases of the Arabian Peninsula. Accurate and rapid detection methods are needed to differentiate MDR strain from other strains of the species. This review summarises the logical relation, prevalence, emerging status and associated mortality of MDR microbes in the Arabian Peninsula.

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Molecular Detection of Cholera Infection during the Outbreak in Thi-Qar Province/Iraq in 2015-2016

Cited by 2 publications

References 22 publications

Phylogenetic Analysis of Biofilm Association Protein (BapA) Amplicons in Salmonella spp. Isolated from Broiler (Ross 308) in Thi-Qar Province/Iraq

Phylogenetic Analysis of Biofilm Association Protein (BapA) Amplicons in Salmonella spp. Isolated from Broiler (Ross 308) in Thi-Qar Province/Iraq

Emerging Status of Multidrug-Resistant Bacteria and Fungi in the Arabian Peninsula

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