Molecular detection of bovine and porcine Torque teno virus in plasma and feces

Brassard, Julie; Gagné, Marie-Josée; Lamoureux, Lisyanne; Inglis, G. Douglas; Leblanc, Danielle; Houde, Alain

doi:10.1016/j.vetmic.2007.07.014

Cited by 67 publications

(53 citation statements)

References 21 publications

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“…15 A number of researchers have reported TTV prevalence in various situations. 6,8 Coinfection with different genogroups of TTV in an individual was found to be common. 12 TTV genogroup 2 has been found more frequently in Porcine circovirus-associated disease (PCVAD)-affected pigs than in TTV genogroup 1, 9 although TTV genogroup 2 was also prevalent in European wild boars.…”

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confidence: 99%

Quantitative Detection of Porcine Torque Teno Virus in Porcine Circovirus-2–Negative and Porcine Circovirus–Associated Disease-Affected Pigs

et al. 2010

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Abstract. Torque teno virus (TTV) is a recently identified virus that has a wide range of host tropisms from humans to shrews. Human TTV and Torque teno mini virus are distributed worldwide, and their high prevalence in human populations has been reported. Pigs have their own species-specific TTV, and like human TTV, a high prevalence of porcine TTV also has been reported. Despite its high prevalence, the role of TTVrelated disease or syndrome in humans and pigs has not been determined. In the swine industry, TTV is thought to be one of the agents that aggravate clinical manifestation of Porcine circovirus-associated disease (PCVAD), a newly emerging, economically devastating disease. The purpose of the current study was to quantify TTV viral load in serum obtained from Porcine circovirus-2-negative pigs and PCVAD-affected pigs with real-time quantitative polymerase chain reaction assays and to compare TTV viral load between these groups. Results of this study indicate that there are no statically remarkable differences in TTV viral load between the 2 groups, which indicates that TTV might not be an agent of aggravation in PCVAD.

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confidence: 99%

Quantitative Detection of Porcine Torque Teno Virus in Porcine Circovirus-2–Negative and Porcine Circovirus–Associated Disease-Affected Pigs

et al. 2010

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“…Previous studies reported higher rates for TTSuV in fecal samples, suggesting that the fecal-oral route is an important route of TTSuV transmission (Brassard et al 2008, Leme et al 2012). …”

Section: Discussionmentioning

confidence: 95%

“…This virus was first isolated from a Japanese man with non A-E post-transfusional hepatitis (Nishizawa et al 1997). Since then, TTV has been shown to infect humans, non-human primates, and farm animals (Leary et al 1999, Okamoto et al 2000, 2001, 2002, Brassard et al 2008.…”

Section: Introductionmentioning

confidence: 99%

Torque teno sus virus (TTSuV) infection at different stages of pig production cycle

Leme

Alfieri

2013

Pesq. Vet. Bras.

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Pesq. Vet. Bras. 33 (7) It has been demonstrated that TTSuV might increase the severity of other important viral diseases with economic and public health impacts. At present, there is no information on the age distribution of pigs infected with TTSuV in Brazilian herds. This study evaluated the frequency of TTSuV infection in pigs at different stages of production. Fecal samples (n=190) from pigs at 1 to 24 weeks of age and from breeders at 6 farrow-to-weaning (up to 8 weeks of age) and 9 grower-to-finish (9 weeks of age onwards) farms in the western region of Paraná state, Brazil, were evaluated by PCR. Fragments of the 5' UTRs of TTSuV1 and/or TTSuVk2 DNAs were identified in 126 (66.3%) of the fecal samples. Significant differences were found with the percentages of positive samples for TTSuV1, TTSuVk2, and mixed infections by both genera between and within the different pig production stages. Fecal samples from the grower-to-finish farms had TTSuV detection rates (90.1%; 64/71) that were significantly (p<0.05) higher than those from the farrow-to-weaning farms (52.1%; 62/119). TTSuV detection was significantly (p<0.05) more frequent in finisher pigs than in the animals from the other stages. The UTR nucleotide sequences in this study presented higher similarities to strains from Norway (96%, TTSuV1), and Argentina and China (97.1%, TTSuVk2). These results suggest that TTSuV infection has spread to pigs of all production stages and that the viral infection rate increases with the age of the animals. In the western region of Paraná state, Brazil, TTSuV1 and TTSuVk2-induced infections were more frequently observed in suckling piglets and finisher pigs, respectively. Phylogenetic analysis pointed out the possibility of different strains of TTSuV1 and TTSuVk2 circulating in pig herds of Brazil. há informações sobre a distribuição da infecção pelo TTSuV, de acordo com a faixa etária, em rebanhos suinícolas brasileiros. Este estudo avaliou a frequência da infecção pelo TTSuV nas diferentes categorias de produção de suí-nos. Amostras fecais (n=190) de suínos com 1 a 24 semanas de idade e de reprodutores provenientes 6 unidades produtoras de leitão (até 8 semanas de idade) e 9 unidades de terminação (9 semanas de idade em diante) da região oeste do Paraná, Brasil, foram avaliadas pela técnica de PCR. Fragmentos da região 5' UTR do DNA do TTSuV1 e/ ou TTSuVk2 foram identificados em 126 (66,3%) amostras fecais. Diferenças significativas foram encontradas em relação às porcentagens de amostras positivas para o TTSuV1, Pesq. Vet. Bras. 33 (7)

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“…On the other hand, TTSuV2 was detected in all pSE (100%, 8/8) at 14 days of age but showed a reduced value of 62.5 (5/8) at 30 days of age; however, the virus was detected in all pSE (100%, 8/8) [3][4][5][6][7][8] in more than one sampling times and showed consecutive PCR positivity in at least 2 sampling ages. On the contrary, 62.5% (5 out of 8) of pSE were TTSuV2 PCR positive (No.…”

mentioning

confidence: 92%

“…TTSuV1 and TTSuV2 (TTSuVs) have been detected from serum, plasma, semen, faeces, colostrum, nasal secretion and various tissues [5,8,13,26,29,30] exclusively by Polymerase chain reaction (PCR), and different pig rearing countries have reported prevalence rate ranging from 24 to 100% [4,8,[14][15][16][17][24][25][26]. Limited longitudinal studies employing serum [17,26] and cross-sectional studies employing serum and tissues [1,8,9,27] have shown that TTSuV infection increases with age.…”

mentioning

confidence: 99%

Infection Dynamics of Torque Teno Sus Virus Types 1 and 2 in Serum and Peripheral Blood Mononuclear Cells

Tshering

Takagi

Deguchi

2012

The Journal of Veterinary Medical Science

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ABSTRACT. This study was carried out to investigate the presence of Torque teno sus virus types 1 (TTSuV1) and 2 (TTSuV2) in a longitudinally (14 to 150 days of age) collected paired pooled sera (pSE) and peripheral blood mononuclear cells (pPBMCs) using nested polymerase chain reaction. The detection rate of TTSuV1 in pSE increased from 14 to 90 days of age, but a progressive decline was observed from 120 to 150 days of age, while in pPBMC, a high value was maintained till the end of growing-finishing period. On the contrary, except in PBMCs at 30 days of age, high detection rates of TTSuV2 were found in both pSE and pPBMCs in all sampling ages. The detection rate of TTSuVs between pSE and pPBMCs was positively correlated at all sampling ages except for TTSuV1 at 150 days of age. This is the first study showing the presence of TTSuVs in PBMCs from pigs and describing the in vivo infection dynamics of TTSuV in paired sera and PBMCs during the entire growing and finishing periods of pigs reared in conventional farms.

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Molecular detection of bovine and porcine Torque teno virus in plasma and feces

Cited by 67 publications

References 21 publications

Quantitative Detection of Porcine Torque Teno Virus in Porcine Circovirus-2–Negative and Porcine Circovirus–Associated Disease-Affected Pigs

Quantitative Detection of Porcine Torque Teno Virus in Porcine Circovirus-2–Negative and Porcine Circovirus–Associated Disease-Affected Pigs

Torque teno sus virus (TTSuV) infection at different stages of pig production cycle

Infection Dynamics of <i>Torque Teno Sus Virus</i> Types <i>1</i> and <i>2</i> in Serum and Peripheral Blood Mononuclear Cells

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