Molecular Detection of Antibiotic Resistance Determinants in Lactobacillus Bacteria Isolated from Human Dental Plaques

Gharajalar, Sahar Nouri; Firouzamandi, Masoumeh

doi:10.29252/jommid.5.3.4.51

Cited by 2 publications

(2 citation statements)

References 13 publications

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“…β-Lactamases represent the main mechanism of bacterial resistance to β-lactam antibiotics. Some extended-spectrum beta-lactamases (ESBLs) have been detected in clinical isolates of lactobacilli : OXA-48 [ 33 ], bla TEM, bla OXA-1 , bla SHV [ 35 ], and bla CTX-M [ 36 ]. In our previous study, we revealed a wide distribution of the bla TEM gene in Lactobacillus isolates from different sources and detected genes bla SHV and bla OXA-1 in some of them [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

Alarming Antibiotic Resistance of Lactobacilli Isolated from Probiotic Preparations and Dietary Supplements

et al. 2022

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In this study, we screened eight commercially available brands of Lactobacillus-containing probiotic preparations and dietary supplements for resistance towards commonly administered antibiotics of different classes. According to disc diffusion results, most of the isolates were resistant to vancomycin and susceptible to penicillin-type antibiotics (ampicillin and amoxicillin), carbapenems (imipenem, meropenem, and ertapenem), and inhibitors of protein synthesis (chloramphenicol, erythromycin, tetracycline, clarithromycin, and linezolid). However, based on minimum inhibitory concentration (MIC) values, six strains were reconsidered as resistant to tetracycline. All tested lactobacilli were resistant towards amikacin, ciprofloxacin, and norfloxacin. Resistance to cephalosporins was highly variable and decreased in the following order: ceftazidime/cefepime, ceftriaxone, cefotaxime, cefazolin, and cefoperazone. PCR screening for antibiotic resistance determinants in probiotic lactobacilli revealed a wide occurrence of vancomycin resistance gene vanX, ciprofloxacin resistance gene parC, and extended-spectrum β-lactamase gene blaTEM. We also detected the tetK gene for tetracycline resistance in one isolate. Additionally, we identified discrepancies between the claims of the manufacturers and the identified species composition, as well as the enumerated amount of viable bacteria, for several products. The results of this study raise concerns about the safety of lactobacilli for human consumption as probiotics, as they may act as reservoirs of transferable antibiotic resistance genes.

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Section: Discussionmentioning

confidence: 99%

Alarming Antibiotic Resistance of Lactobacilli Isolated from Probiotic Preparations and Dietary Supplements

et al. 2022

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“…Antibiotic disks included Cefotaxime (30µg), Erythromycin (15 µg), Penicillin (10 unit), Chloramphenicol (30 µg), Tetracycline (30 µg), Ceftriaxone (30 µg), Vancomycin (30 µg), and Azithromycin (15 µg) (Padtanteb, Iran). [14,15].…”

Section: Methodsmentioning

confidence: 99%

Antibacterial Activity of Trachyspermum ammi Essential Oil Against Streptococcus mutans Isolated from Human Dental Plaques

Gharajalar

Hassanzadeh²,

Mahmoudi³

et al. 2022

JoMMID

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Introduction: Mutans Streptococci are significant pathogens isolated from dental plaques. Due to the increasing frequency of antimicrobial resistance among pathogens causing dental caries, more studies have focused on using natural agents against them. This study aimed to evaluate the antibacterial effects of Trachyspermum ammi essential oil against Streptococcus mutans isolated from dental plaques. Methods: Twenty human dental plaque samples were collected, and eight S. mutans isolates were detected using biochemical and molecular tests. The antibiotic susceptibility patterns of the isolates were determined by the disc diffusion method. Also, T. ammi essential oil antibacterial activity was investigated using the disc diffusion method and determining minimum inhibitory concentration (MIC50) and minimum bactericidal concentration (MBC) values. Results: Phenotypic and genotypic characterization identified S. mutans in 40% of the samples. The antibiotic susceptibility assay revealed the highest resistance patterns against cefotaxime (100%), ceftriaxone (100%), and penicillin (87.5%). The T. ammi essential oil demonstrated 20 µg/ml MIC and 80µg/ml MBC against S. mutans. Conclusion:The present study revealed a potent antibacterial activity for T. ammi essential oil against S. mutans isolates.

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Molecular Detection of Antibiotic Resistance Determinants in Lactobacillus Bacteria Isolated from Human Dental Plaques

Cited by 2 publications

References 13 publications

Alarming Antibiotic Resistance of Lactobacilli Isolated from Probiotic Preparations and Dietary Supplements

Alarming Antibiotic Resistance of Lactobacilli Isolated from Probiotic Preparations and Dietary Supplements

Antibacterial Activity of Trachyspermum ammi Essential Oil Against Streptococcus mutans Isolated from Human Dental Plaques

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