Molecular detection and phylogenetic tree of echovirus in children at Thi-Qar province Iraq

Abstract

The current study was conducted in Thi-Qar province, southern Iraq, A number of 100 blood samples collected from sick Children in Mohammed Al-Mousawi Hospital for Children (ages between 2 days - 3 years), males and females, during the period from November 2021 to February 2022.This study aimed to detects the presence of Echovirus RNA in patients serum by RT-PCR and compare sequences results of current study isolates with previously described isolates from other region of the world and then draw its phylogenetic tree. Based on the results recorded among 100 child have different symptoms only 9% of patients infected with Echovirus, the results also noted a significant difference in viral prevalence in babies patients. The results from the sequencing analysis indicated that three viral isolates belong to the human echovirus, out of nine positive samples for PCR analysis. The results of the viral sequencing showed that the Iraqi strain is identical to the American isolate, China's isolate and Russia's isolate ,all of them have 100% sequencing identical.