Molecular detection and characterization of Anaplasma platys in dogs and ticks in Cuba

Silva, Cláudia Bezerra da; Santos, Huarrisson Azevedo; Navarrete, Maylín González; Ribeiro, Carla Carolina Dias Uzedo; González, Belkis Corona; Zaldívar, Maykelin Fuentes; Pires, Marcus Sandes; Peckle, Maristela; Costa, Renata Lins da; Vitari, Gabriela Lopes Vivas; Massard, Carlos Luiz

doi:10.1016/j.ttbdis.2016.04.012

Cited by 17 publications

(11 citation statements)

References 38 publications

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“…Anaplasma platys infection has been commonly reported in dogs and is described as being a potential human pathogen (Arraga-Alvarado et al, 2014;Geurden et al, 2018;Maggi, Mascarelli, Havenga, Naidoo, & Breitschwerdt, 2013;Matei et al, 2016). Rhipicephalus sanguineus ticks have been identified as the dominant vector in the transmission of A. platys (Cicuttin et al, 2014;Geurden et al, 2018;Ramos et al, 2014;Silva et al, 2016). Wolbachia spp.…”

Section: Discussionmentioning

confidence: 99%

Screening of ectoparasites from domesticated dogs for bacterial pathogens in Vientiane, Lao PDR

Nguyen¹,

Theppannga²,

Vongphayloth³

et al. 2020

Zoonoses and Public Health

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Arthropod‐borne diseases are widespread worldwide and are a complex interaction between animals, humans and ectoparasites. The understanding of the diversity and epidemiology of organisms transmitted by arthropod vectors, and the role of hosts and vectors in transmission of infections remain limited in Lao PDR. What knowledge does exist is primarily focused on more rural regions of the country. This study screened ectoparasites from domestic dogs in Vientiane city for the presence of bacterial pathogens of zoonotic importance. A total of 3,511 arthropod vectors were collected from 112 dogs. Vectors collected were Rhipicephalus sanguineus ticks, Ctenocephalides felis felis and Ctenocephalides felis orientis fleas and Heterodoxus spiniger lice. A sub‐sample of vectors from each dog was analysed by PCR to identify the potential bacteria. From 129 vector pools, Rickettsia spp. was detected in 6.7% (7/105) pools of ticks, 86.4% (19/22) pools of fleas and both pools of lice. Sequencing analysis confirmed Rickettsia felis in 13 flea pools and one louse pool and Rickettsia asembonensis in six flea pools. Anaplasmataceae was identified in 14.3% (15/105) tick pools and 100% (22/22) flea pools. Sequencing revealed the presence of Anaplasma platys in ticks and Wolbachia pipientis in fleas. Leptospira spp. was detected in one tick and one louse pool, and Brucella spp. was detected in 12.4% (13/105) tick pools. All samples were negative for Bartonella spp., Coxiella burnetii and Borrelia burgdorferi. This is the first study providing evidence of R. asembonensis in fleas in Laos. Results from this study show arthropods are potential vectors to transmit zoonotic infection in Vientiane city, suggesting humans are at risk of zoonotic infections in the city.

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Section: Discussionmentioning

confidence: 99%

Screening of ectoparasites from domesticated dogs for bacterial pathogens in Vientiane, Lao PDR

Nguyen¹,

Theppannga²,

Vongphayloth³

et al. 2020

Zoonoses and Public Health

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“…Other than the similarity between the geographical areas of occurrence, the fact that a significant number of animals were co-infected implies that R. sanguineus is probably capable of transmitting both agents. Studies that aimed to detect the DNA of E. canis and A. platys in both dogs and in ticks that infest these animals, reinforce the involvement of this tick in the transmission cycle of these agents (CICUTTIN et al, 2015;SILVA et al, 2016).…”

Section: Agent ------------------------------------Group-------------mentioning

confidence: 89%

Molecular detection of Ehrlichia canis and Anaplasma platys in dogs from municipality of Belém, State of Pará, Brazil

et al. 2019

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The occurrence of diseases transmitted by ticks in dogs is very frequent in Brazil, among these diseases we can highlight the ehrlichiosis and anaplasmosis, which are caused by Ehrlichia canis and Anaplasma platys, respectively. The objective of this study was to survey the occurrence of these pathogens in blood samples from domiciled and stray dogs from the city of Belém, Pará. Two hundred and seventy six dogs were sampled for convenience, and the DNA extracted from the blood of these animals was submitted to nested-PCR for research of E. canis and A. platys. E. canis DNA was detected in 39.4% (109/276) and A. platys DNA in 23.1% (64/276) of the samples, there was a statistically significant difference between the frequency of these agents (P<0.0001), and there was coinfection in 13.4% (37/276) of animals. The frequency of detection of these parasites was higher in stray dogs than in those domiciled for both E. canis (OR=2.84) and A. platys (OR=10.5). Considering the results, it was possible to conclude that E. canis and A. platys are present in the studied population, with stray dogs being more affected by these parasites.

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“…Additionally, a study by Navarrete et al [ 24 ] revealed an increased risk of Ehrlichia infection in some localities with a history of tick infestation. The presence of A. platys , the agent of canine cyclic thrombocytopenia, was also confirmed by PCR diagnosis in 16% of tested dogs ( n = 100) [ 116 ], and the observation of A. platys -like morulae in dog platelets by using blood smears [ 116 ].…”

Section: Ticks and Tbps Infection In Dogsmentioning

confidence: 96%

“…Concerning other pathogens, molecular characterization of 21 isolates of A. platys detected in dogs in Cuba was performed based on gltA and 16S rRNA genes [ 116 ]. Likewise, eight isolates of E. canis detected in ticks fed on dogs were assessed using the16S rRNA sequences [ 117 ].…”

Section: Genetic Variability Of a Marginale Amentioning

confidence: 99%

“…Three different A. platys genotypes were identified in the dog blood samples. Some of the isolates of A. platys had a common ancestry with an African isolate, while others were related to isolates worldwide, suggestive of possible multiple introductions of this pathogen in Cuba with the influx of people, animals, and products [ 116 ]. Concerning Babesia sp.…”

Section: Genetic Variability Of a Marginale Amentioning

confidence: 99%

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Ticks and Tick-Borne Diseases in Cuba, Half a Century of Scientific Research

et al. 2020

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Ticks and the vast array of pathogens they transmit, including bacteria, viruses, protozoa, and helminths, constitute a growing burden for human and animal health worldwide. In Cuba, the major tropical island in the Caribbean, ticks are an important cause of vector-borne diseases affecting livestock production, pet animal health and, to a lesser extent, human health. The higher number of tick species in the country belong to the Argasidae family and, probably less known, is the presence of an autochthonous tick species in the island, Ixodes capromydis. Herein, we provide a comprehensive review of the ticks and tick-borne pathogens (TBPs) affecting animal and human health in Cuba. The review covers research results including ecophysiology of ticks, the epidemiology of TBPs, and the diagnostic tools used currently in the country for the surveillance of TBPs. We also introduce the programs implemented in the country for tick control and the biotechnology research applied to the development of anti-tick vaccines.

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Molecular detection and characterization of Anaplasma platys in dogs and ticks in Cuba

Cited by 17 publications

References 38 publications

Screening of ectoparasites from domesticated dogs for bacterial pathogens in Vientiane, Lao PDR

Screening of ectoparasites from domesticated dogs for bacterial pathogens in Vientiane, Lao PDR

Molecular detection of Ehrlichia canis and Anaplasma platys in dogs from municipality of Belém, State of Pará, Brazil

Ticks and Tick-Borne Diseases in Cuba, Half a Century of Scientific Research

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