Molecular Detection and Characterization of Salmonella Spp. Isolated From Fresh Fishes Sold in Selected Upazila Markets of Bangladesh

Abstract

Aquatic environments are the major reservoirs of Salmonella. Therefore, fishery products have been recognized as a major carrier of food-borne organism. Fish is known to harbor bacteria of public health significance. Aquatic environments are known to influence the bacterial loads in the harvested fish. The present work was undertaken for molecular detection and characterization of Salmonella species isolated from fresh fishes sold in different markets of Jamalpur,Tangail, Kishoreganj and Netrokona districts of Bangladesh. The isolates were identified by their morphological, cultural and biochemical characteristics with standard reference organisms, and molecular methods. Out of 20 pangas fish (Pangasius spp.) samples the number of samples found to be positive for Salmonella spp. was 14 (70%); of 20 koi fish (Anabas spp.) samples this number was 17 (85%); and of 20 tilapia fish (Oreochromis spp.) samples it was 15 (75%). All the isolates of Salmonella were confirmed by targeting genus specific histidine transport operon gene. Antimicrobial susceptibility test was performed to know the susceptibility and resistance patterns of the isolates to different antimicrobial agents. Results of antimicrobial susceptibility test shows that 40 (86.95%) isolates were found to be resistant to azithromycin, 42 (91.30%) were resistant to erythromycin. On the other hand all isolates were 100% susceptible to ciprofloxacin and gentamicin, 38 (82.62%) were susceptible to norfloxacin, 40 (86.95%) were susceptible to streptomycin.

Antimicrobial resistance profiles of salmonella spp. and escherichia coli isolated from fresh nile tilapia (oreochromis niloticus) fish marketed for human consumption

“…Higher prevalence of Salmonella spp. of 64.9% and 75% were also reported previously in tilapia imported from Thailand [ 54 ] and fresh tilapia samples collected from different markets in Bangladesh [ 55 ], respectively. Various countries have different hygienic control and management programs, which may explain the differences in Salmonella spp.…”

Molecular Detection and Antibiotyping of Multidrug-Resistant Salmonella Isolated from Houseflies in a Fish Market

“…Tetracycline-, amoxicillin-, and azithromycin-resistant Salmonella spp. were previously detected in fish in Bangladesh [29]. No published data are yet available on AMR-associated houseflies from fish markets in Bangladesh.…”

“…Moreover, antibiotic-resistant Salmonella spp. were also detected in some indigenous fishes in Bangladesh [29]. Therefore, this study was conducted to investigate whether flies persistent in the fish market area carry antibiotic-resistant Salmonella spp.…”

Antibiotic Susceptibility Profile and Nutritional Evaluation of Raw and Cooked Meat of Cyprinus Carpio

“…Antibiotic-resistant bacteria isolated from fish are quite common, and these antibiotic-resistant, beta-lactamase-producing foodborne bacteria might be transferred to food handlers and consumers. Uncooked fish have high nutritional content but more contamination of bacterial pathogens, while cooked fish meat contains fewer bacterial pathogens and less nutritional value (Seel et al, 2016) Kousar et al (2019).…”

“…In the kingdom, Animalia fish is the largest group, having a population of approximately 30,000 wellknown species and used mainly for oil and food production (Seel et al, 2016;Suseno et al, 2014). Fish consumption as food is recommended because of its good digestibility and highly rich polyunsaturated fatty acids (PUFA).…”