Molecular Detection and Antibiogram of Bacillus cereus Isolated from Dairy Goat with Mastitis in Malaysia

Ghazali, Mohd Faizal; Sukiman, M.Z.; Chai, M.H.; Mohamad, Noor Muzamil; Ariffin, S.M. Zainal

doi:10.1016/j.ijid.2021.12.149

Cited by 4 publications

(5 citation statements)

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“…Furthermore, Hayat et al [35] determined that B. cereus is associated with subclinical mastitis in buffaloes in swats, with a (3.27%) prevalence. In addition, Ghazali et al [36] identified that 23 of 78 milk samples from subclinical mastitic goats contained B. cereus. These variations in results can be attributed to weather variations or the hygiene conditions in the farms that differ from those observed in this study.…”

Section: Resultsmentioning

confidence: 99%

Phenotypic diagnosis and genotypic identification of Bacillus cereus causing subclinical mastitis in cows

Eid¹,

Aref²,

Ibrahim

2023

Vet World

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Background and Aim: Bovine mastitis is a disease that affects dairy cows and impacts the global dairy industry. Bacillus spp. can infect the mammary gland during lactation, intramammary treatment, or dry cow therapy. This study aimed to isolate and identify Bacillus spp. in raw milk samples from cows with subclinical mastitis from dairy farms in Beheira, Giza, Alexandria, and Menoufia Governorate, Egypt. We also investigated their antibiotic sensitivity and detected the enterotoxigenic and antibiotic resistance genes. Materials and Methods: A total of 262 milk samples (15-20 ml each) were examined microscopically, biochemically, and phenotypically. A polymerase chain reaction was used for genotypic identification and detecting antibiotic-resistance and enterotoxigenic genes. Antibiotic sensitivity was tested using the agar well diffusion test. Results: Bacillus cereus was identified in 47.7% of samples. Nhe and hblD enterotoxin genes were found in 93.64% (103/110) and 91.82% (101/110) of the samples, respectively. Tetracycline and β-lactam antibiotic-resistance genes were present in 50% (55/110) and 98.18% (108/110), respectively, of the samples. All isolates were resistant to cefepime, cefixime, and oxacillin, while they were susceptible to amoxicillin-clavulanic, chloramphenicol, ampicillin/sulbactam, and levofloxacin. Conclusion: These results highlight the need to promote awareness regarding B. cereus, the most common pathogen causing mastitis in Egyptian dairy cows. We also emphasized that antibiotic misuse during mastitis is a potential public health threat. Keywords: antibiotics, dairy cows, Egypt, lactation.

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Section: Resultsmentioning

confidence: 99%

Phenotypic diagnosis and genotypic identification of Bacillus cereus causing subclinical mastitis in cows

Eid¹,

Aref²,

Ibrahim

2023

Vet World

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“…(24). Klinik ve subklinik mastitisli keçilerden elde edilen 307 süt örneğinin 78'inden izole elde edilen B. cereus'un test edilen amoksisilin/klavulonik asit, sefotoksin ve oksasilin antibiyotiklerine karşı %100 oranında direnç geliştirdiği tespit edilmiştir (23). Yıbar ve ark.…”

Section: Bulgularunclassified

“…Diğer yandan Ghazali ve ark. (23) tarafından süt izolatlarından yapılan bir diğer çalışmada ise sulphametoksazol/trimetoprime karşı %100 oranında direnç tespit edilmiştir. Fiedler ve ark.…”

Section: Bulgularunclassified

“…cereus, insanların yanı sıra hayvanlarda da özellikle mastitis ve yara enfeksiyonlarına yol açmaktadır. Çok sayıdaki çalışmada sığırlarda akut gangrenoz mastitis etkeni olarak bildirilen B. cereus' un, antibiyotik tedavisi, cerrahi müdehaleler veya meme başındaki yaralar kaynaklı enfeksiyona yol açtığı (21), sığırlarda, keçilerde mastitis olgularından sorumlu olduğu (22)(23)(24) bildirilmiştir. Halk sağlığı açısından bakıldığında, doğada yaygın olarak bulunan B. cereus etkeninin yol açtığı bu enfeksiyonlar sebebiyle özellikle çiğ süt ürünleri aracılığıyla da insanlar tarafından alınması ihtimal dahilindedir.…”

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Determination of Susceptibility Profiles of B. cereus Strains towards Certain Antibiotics Isolated from Plant-Derived Foods

YALÇIN,

ÇÖL,

AKSU

2023

Dicle Üniv Vet Fak Derg

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Bacillus cereus, gram pozitif, fakültatif anaerob, sporlu bir bakteridir. Doğada yaygındır ve baharat, et, süt, tahıl, bakliyat gibi gıdalar ile hayvanlarda mastitis olgularından ve yara enfeksiyonlarından izole edilmektedir. Aktarılabilir antibiyotik direnç genlerinin gıda zincirinde saptanmasıyla ilgili çalışmalar Dünya Sağlık Örgütü’nün bakterilerde antibiyotik direnç gelişimi ve yayılımını 21. yüzyılın önemli sağlık sorunlarından biri olarak bildirilmesiyle önem kazanmıştır. Mevcut çalışma bitkisel kaynaklı çeşitli gıdalardan izole edilen B. cereus suşlarının farklı grup antibiyotiklere karşı fenotipik duyarlılık profillerinin belirlenmesi amacıyla gerçekleştirilmiştir. Toplam 152 bitkisel kaynaklı gıdada B. cereus %19.7 oranında ve 1.4x10² kob/g ile 1.0x10⁴ kob/g arasında saptanmıştır. İzole edilen 30 B. cereus suşunun 8 farklı grup antibiyotiğe karşı fenotipik duyarlılık profilleri disk difüzyon yöntemiyle test edilmiştir. Sonuçlar Avrupa Antimikrobiyal Duyarlılık Testi Komitesi (EUCAST) kriterlerine göre duyarlı, orta duyarlı veya dirençli olarak yorumlanmıştır. B. cereus suşlarının tamamı sefiksim ve amoksisilin/klavulonik asite dirençli iken, %3.4’ü sulphametoksazol/trimetoprime karşı duyarlı, %6.6’sı orta derecede duyarlı, %90’nının ise dirençli olduğu saptanmıştır. En yüksek antibiyotik duyarlılık (%100) kloramfenikol ve (%93.3) gentamisine karşı iken, tetrasiklin ve eritromisine eşit oranda (%56.7) duyarlılık belirlenmiştir. Suşların %83.3’ünün siprofloksasine orta derecede duyarlı, %16.7’sinin ise dirençli olduğu saptanmıştır. Sonuç olarak; antibiyotik duyarlılık profillerinin gıda kaynaklı patojenlerde belirlenmesinin antimikrobiyal direnç geni yayılımlarının gıda kaynaklı patojenler aracılığıyla da mümkün olması, uygun tedavi planlarının oluşturulması ve antibiyotik direnç dağılımlarının izlenebilmesi açısından önemli olduğu düşünülmektedir.

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“…It contaminates rice-based products, fresh vegetables, meat, and dairy products. Bacillus cereus also causes mastitis in dairy cows [ 2 ] and goats [ 3 ]. Staphylococcus aureus is a Gram-positive pathogen and a causal agent of several infectious diseases ranging from skin infections to life-threatening.…”

Section: Introductionmentioning

confidence: 99%

Robusta coffee extracts inhibit quorum sensing activity in Chromobacterium violaceum and reduce biofilms against Bacillus cereus and Staphylococcus aureus

et al. 2022

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Background and Aim: Bacillus cereus and Staphylococcus aureus cause foodborne intoxication in humans and animals. Pathogens can produce biofilms controlled by the quorum sensing system. The study aimed to investigate the antibacterial, antibiofilm, and anti-quorum sensing activities of Coffea canephora P. ex Fr. (Robusta coffee) extracts against B. cereus and S. aureus. Materials and Methods: Ethanol extracts of fruit peels and seeds of Robusta coffee were tested for antibacterial activity against B. cereus and S. aureus using a broth microdilution assay. Reduction of the biofilm formation and elimination of the viability of mature biofilm-grown cells of B. cereus and S. aureus were determined. Inhibition of quorum sensing activity in Chromobacterium violaceum by the extracts was investigated using the disk diffusion method and flask incubation assay. Results: Fresh fruit peel extract showed the strongest antibacterial activity against B. cereus and S. aureus with minimum inhibitory concentration (MIC) values of 2 and 4 mg/mL, respectively. However, the extracts did not inhibit Escherichia coli, avian pathogenic E. coli, and Pseudomonas aeruginosa at 8 mg/mL. Significant inhibition of biofilm formation at 1/2 × MIC of the fresh peel extract was detected in B. cereus (56.37%) and S. aureus (39.69 %), respectively. At 8 × MIC of the fresh peel extract, a significant elimination of the mature biofilm viability was detected in B. cereus (92.48%) and S. aureus (74.49%), respectively. The results showed that fresh and dried peel fruit extracts at 1/2 × MIC significantly reduced violacein production with the highest percentage inhibition ranging from 44.53 to 47.48% at 24 h (p ≤ 0.05). Conclusion: The results of the present study suggest the potential therapeutic benefits of Robusta coffee extracts in inhibiting the growth, biofilm, and quorum sensing of both B. cereus and S. aureus. The results put forward an alternative strategy to control the foodborne intoxications caused by both pathogens.

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Molecular Detection and Antibiogram of Bacillus cereus Isolated from Dairy Goat with Mastitis in Malaysia

Cited by 4 publications

References 0 publications

Phenotypic diagnosis and genotypic identification of Bacillus cereus causing subclinical mastitis in cows

Phenotypic diagnosis and genotypic identification of Bacillus cereus causing subclinical mastitis in cows

Determination of Susceptibility Profiles of B. cereus Strains towards Certain Antibiotics Isolated from Plant-Derived Foods

Robusta coffee extracts inhibit quorum sensing activity in Chromobacterium violaceum and reduce biofilms against Bacillus cereus and Staphylococcus aureus

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