Molecular Cytogenetic Study of the Nf2 Gene Deletion in Meningioma in Sudanese Patients

Abstract

Meningioma is the second most common adult central nervous system tumor. Mutations and/or deletions within the tumor suppressor gene neurofibromatosis type 2 (NF2) are associated with meningioma development and progression. We studied 29 meningioma samples by cytogenetic analysis and interphase fluorescence in situ hybridization (I-FISH) using a locus-specific probe for the NF2 gene region. We detected loss of the NF2 gene in all samples except for one. In 10 of the 29 samples, karyotypic analyses confirmed the I-FISH results and revealed additional numerical and/or structural rearrangements in nine of them. Our study confirmed: i) the limited role of banding cytogenetics in assessing chromosomal rearrangements in meningioma, as this tumor is hard to be grown in cell culture; ii) we could show that two-color I-FISH is well-suited for NF2-deletion screening. Our results were in accordance with those of comparable studies, even though the frequency of 97.0% of meningiomas with NF2 deletions is exceptionally high in the studied Sudanese patients.

New insights into the genomic landscape of meningiomas identified FGFR3 in a subset of patients with favorable prognoses

“…NF2 -mutated meningiomas show higher chromosomal instability during progression than non- NF2 mutated meningiomas [27–32]. In this study, tumors with NF2 mutations harbored 1.83 GAs per patient compared to the average of 1.24 GAs in non- NF2 mutations, consistent with this instability.…”

Frequency of the Loss of Heterozygosity of the NF2 Gene in Sporadic Spinal Schwannomas

“…Few cells presented monosomy of chromosome 22. This is different from meningiomas, which may present deletions or monosomy in isolation in different samples of the tumor (20,32). We know of no other studies in which a two-color FISH was used to analyze sporadic schwannomas.…”

“…The DNA derived from the BAC-probe RP11-551L12, which is specific to the NF2 gene (22q12.2), was labeled with Texas Red, while the BCR Spectrum Green Probe located at 22q11.2 (Abbott Laboratories, Illinois, USA) served as an internal control. The FISH procedure was conducted according to the standard protocols (19,20). For microscopic evaluation, 200 interphase nuclei were examined in each specimen.…”

Enhanced anticancer activity of DM1-loaded star-shaped folate-core PLA-TPGS nanoparticles

“…After trypsinization and washing with PBS, the cells were fixed with methanol-acetic acid (3:1) and air-dried on slides. A bacterial artificial chromosome (BAC) clone specific to Her2 DNA was labeled with dUTP-FITC (Fermentas, Burlington, Canada) and the chromosome 17 centromere probe (p17H8) was labeled with chromatide Alexa Fluor 594-5-dUTP (Invitrogen, Carlsbad, CA, USA) using nick translation, and FISH was performed as described previously [ 31 ].…”