Molecular comparison of Neospora caninum oocyst isolates from naturally infected dogs with cell culture-derived tachyzoites of the same isolates using nested polymerase chain reaction to amplify microsatellite markers

First in vitro isolation of Besnoitia besnoiti from chronically infected cattle in Germany

“…In addition, we also used the NA42/13 cell line and primary embryonic bovine cells (KH-R), previously applied to in vitro isolate N. caninum or Hammondia spp. (Schares et al, 2003(Schares et al, , 2005Basso et al, 2009).…”

Neosporosis in animals—The last five years

“…Clinical disease maybe associated with the strain of N. caninum. Isolates of N. caninum from various hosts are genetically similar although many strains have their own molecular signature as determined by multilocus microsatellite analysis (Regidor-Cerrillo et al, 2006;Al-Qassab et al, 2009aBasso et al, 2009bBasso et al, , 2010. The molecular characteristics of a strain could be useful for epidemiological studies.…”

Genetic diversity of bovine Neospora caninum determined by microsatellite markers

“…The Jaccard's similarity index analysis (Fig. 1) showed that these two allelic profiles differed significantly from each other and from other isolates of previous studies (Regidor-Cerrillo et al 2006and, 2012Al-Qassab et al 2009;Basso et al, 2009). No relationship could be established among strains from the different geographical origins.…”

“…Recently, microsatellites (short, tandem repeats with 2-6 bases long), have represented another class of genetic markers (Ajzenberg et al 2001, De Luca et al 2002. Using microsatellite markers, Regidor-Cerrillo et al (2006, 2012and 2013, Basso et al (2009), Pedraza-Díaz et al (2009) and Al-Qassab et al (2009 described different genetic variation among N. caninum derived from European countries, United States, Argentina and Japan. Although, N. caninum strains have been isolated in Iran (NC-Iran) (Salehi et al, 2012) and many molecular and serological studies have been carried out on neosporesis (Haddadzadeh, et al, 2007, Razmi et al, 2007 A C C E P T E D M A N U S C R I P T…”