Molecular cloning, tissue expression and protein structure prediction of the porcine 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) gene

Xiao, Chen; Li, Zongjin; Kong, Lujun; Liu, Guifen; Fu, Jinluan; Wang, Aiguo

doi:10.1016/j.gene.2011.12.051

Cited by 17 publications

(14 citation statements)

References 31 publications

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“…Steers had lower HMGCR mRNA levels in the liver. HMGCR is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism, mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase ( Chen et al, 2012 ). Thus, lower expression of HMGCR in steers may reflect higher circulating cholesterol concentrations through a negative feedback mechanism.…”

Section: Discussionmentioning

confidence: 99%

Effects of Castration on Expression of Lipid Metabolism Genes in the Liver of Korean Cattle

Baik

Nguyen

Jeong

et al. 2014

Asian Australas. J. Anim. Sci

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Castration induces the accumulation of body fat and deposition of intramuscular fat in Korean cattle, resulting in improved beef quality. However, little is known about the metabolic adaptations in the liver following castration. To understand changes in lipid metabolism following castration, hepatic expression levels of lipid metabolism genes were compared between Korean bulls and steers. Steers had higher (p<0.001) hepatic lipids contents and higher (p<0.01) mRNA levels of lipogenic acetyl-CoA carboxylase. This differential gene expression may, in part, contribute to increased hepatic lipid content following the castration of bulls. However, we found no differences in the hepatic expression levels of genes related to triglyceride synthesis (mitochondrial glycerol-3-phosphate acyltransferase, diacylglycerol O-acyltransferase 1 and 2) and fatty acid (FA) oxidation (carnitine palmitoyltransferase 1A, C-4 to C-12 straight chain acyl-CoA dehydrogenase, very long chain acyl-CoA dehydrogenase) between bulls and steers. No differences in gene expression for very-low-density lipoprotein (VLDL) secretion, including apolipoprotein B mRNA and microsomal triglyceride transfer protein (MTTP) protein, were observed in the liver although MTTP mRNA levels were higher in steers compared to bulls. In conclusion, FA synthesis may contribute to increased hepatic lipid deposition in steers following castration. However, hepatic lipid metabolism, including triglyceride synthesis, FA oxidation, and VLDL secretion, was not significantly altered by castration. Our results suggest that hepatic lipid metabolism does not significantly contribute to increased body fat deposition in steers following castration.

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Section: Discussionmentioning

confidence: 99%

Effects of Castration on Expression of Lipid Metabolism Genes in the Liver of Korean Cattle

Baik

Nguyen

Jeong

et al. 2014

Asian Australas. J. Anim. Sci

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“…The trans-membrane domain of protein can serve as a membrane receptor for membrane-anchored protein and ion channel protein (Chen et al, 2012a). Grass carp HMGR is also a trans-membrane protein (the trans-membrane domain at the N-terminal 20-37, 57-79, 91-113, 157-179 and 192-214, respectively).…”

Section: Discussionmentioning

confidence: 99%

“…Cholesterol acts as an important precursor for several physiological compounds such as steroid hormones, corticoids and bile acids (Sheen, 2000;Chen et al, 2012a). Although it is not an essential nutrient for fish, it plays important physiological roles.…”

Section: Introductionmentioning

confidence: 99%

“…3-Hydroxy-3-methylglutaryl-Coenzyme A reductase (HMGR) is a rate-limiting enzyme for cholesterol biosynthesis, catalyzing conversion of HMG-CoA to mevalonic acid (Norambuena et al, 2013). It has been reported that HMGR is a good marker of stress response and tissue damage in fish because of its conversed N-and C-terminus portions (Chen et al, 2012a;Gornati et al, 2005). However, to date there is very little available information on fish HMGR.…”

Section: Introductionmentioning

confidence: 99%

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Ye¹,

Huang²,

Ye³

2018

Turk. J. Fish. Aquat. Sci.

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Abstract3-Hydroxy-3-methylglutaryl-Coenzyme A reductase (HMGR) is a rate-limiting enzyme for cholesterol biosynthesis. The aim of this study was to identify HMGR molecular characterization in grass carp (Ctenopharyngodon idellus), and to evaluate regulation of its expression by oxidized fish oil. Specific primers were designed for 3'RACE and 5'RACE to gain HMGR cDNA sequence of grass carp and to clone full-length HMGR cDNA. This cDNA covered 3591 bp, with 2526 bp for the open reading frame (ORF), 77 bp for 5'-untranslated region (UTR) and 988 bp for 3'UTR. ORF encoded a protein consisting of 841 amino acids. The result revealed that HMGR mRNA is predominantly expressed in liver. Multiple alignments and phylogenetic analysis indicated that grass carp HMGR has a high degree of conservation with other fish species. A 10-week feeding trial was conducted to investigate effects of offering diets containing fresh and oxidized fish oil on HMGR expression in liver and intestine of grass carp. The results showed that HMGR expression in fish fed the diet containing oxidized fish oil was significantly higher than fish fed the fresh fish oil after 10, 20, 30 and 70 days of feeding. Serum diamineoxidase (DAO) activity of fish was also increased by oxidized fish oil.

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“…However, Kim et al (2011) found that methyl jasmonate treatment reduced the expression of CAS gene in the roots of wild Bupleurum falcatum. HMGR, catalyzing the conversion of HMG-CoA to mevalonic acid, is considered as a rate-limiting enzyme in cholesterol synthesis, and it also plays a critical role in controlling isoprenoid derivatives related pathways (Chen et al 2012). Cools et al (2011) found that ethylene treatment could increase in the expression of HMGR gene in onion.…”

Section: Discussionmentioning

confidence: 99%

Ethylene treatment improves diosgenin accumulation in in vitro cultures of Dioscorea zingiberensis via up-regulation of CAS and HMGR gene expression

Diarra¹,

He²,

Wang³

et al. 2013

Electron. J. Biotechnol.

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Background:The perennial medicinal herb Dioscorea zingiberensis is a very important plant used for steroid drug manufacturing for its high level of diosgenin in rhizome. Although the stimulation of diosgenin accumulation by ethylene has been reported in a few of plant species, its regulation is not yet characterized at the molecular level, the underlying molecular mechanism remains elusive. Results: In this study, the effects of ethylene on diosgenin biosynthesis in in vitro cultures of D. zingiberensis were described. The results showed that, in samples treated with ethylene at concentration E3 (10 4 dilution of 40% ethephon), the diosgenin biosynthesis was significantly promoted in comparison with the control samples. Treatment with high concentrations of ethylene had inhibitory effect, whereas with low concentration of the gas elicitor brought about no detectable deleterious effect on the growth rate and diosgenin content of the cultures. The considerable increase of diosgenin level in in vitro cultured Dioscorea zingiberensis by ethylene application is accompanied by the concomitant increase of soluble proteins and chlorophyll content. The gene expressions of cycloartenol synthase and 3-hydroxy-3-methylglutaryl-CoA reductase but not of squalene synthase or farnesyl pyrophosphate synthase were up-regulated by applied ethylene. Conclusions: Our results suggest that ethylene treatment enhanced diosgenin accumulation via upregulation of the gene expressions of cycloartenol synthase and 3-hydroxy-3-methylglutaryl-CoA reductase.

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Molecular cloning, tissue expression and protein structure prediction of the porcine 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) gene

Cited by 17 publications

References 31 publications

Effects of Castration on Expression of Lipid Metabolism Genes in the Liver of Korean Cattle

Effects of Castration on Expression of Lipid Metabolism Genes in the Liver of Korean Cattle

Untitled

Ethylene treatment improves diosgenin accumulation in in vitro cultures of Dioscorea zingiberensis via up-regulation of CAS and HMGR gene expression

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