Molecular Cloning, Segmental Distribution and Ontogenetic Regulation of Cationic Amino Acid Transporter 2 in Pigs

Zou, Shigeng; Zhi, Ai-Nlin; Zhou, Xuyang; Zuo, Jianjun; Zhang, Yan; Huang, Zhiyi; Xu, Pingwen; Feng, Dan

doi:10.5713/ajas.2009.80469

Cited by 3 publications

References 22 publications

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Expression of cationic amino acid transporters in pig skeletal muscles during postnatal development

et al. 2017

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The cationic amino acid transporter (CAT) protein family transports lysine and arginine in cellular amino acid pools. We hypothesized that CAT expression changes in pig skeletal muscles during rapid pig postnatal development. We aimed to investigate the tissue distribution and changes in the ontogenic expression of CATs in pig skeletal muscles during postnatal development. Six piglets at 1, 12, 26, 45, and 75 days old were selected from six litters, and their longissimus dorsi (LD), biceps femoris (BF), and rhomboideus (RH) muscles, and their stomach, duodenum, jejunum, ileum, colon, liver, kidney, heart, and cerebrum were collected. CAT-1 was expressed in all the 12 tissues investigated. CAT-2 (CAT-2A isoform) expression was highest in the skeletal muscle and liver and lowest in the jejunum, ileum, kidney, and heart. CAT-3 was expressed mainly in the colon and detected in the jejunum, ileum, and cerebrum. The CAT-1 expression was higher in the skeletal muscle of day 1 pigs than in that of older pigs (P < 0.05). The CAT-2 mRNA level was lowest at day 1, but increased with postnatal development (P < 0.05). There was no significant change in CAT-1 expression among the LD, BF, and RH during postnatal development (P > 0.05); however, there was a change in CAT-2 expression. The CAT-2 expression was highest in the LD of 12-, 26-, 45-, and 75-day-old pigs, followed by the BF and RH (P < 0.05). These results suggest that CAT-1 and CAT-2 play different roles in pig skeletal muscles during postnatal development.

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Expression of cationic amino acid transporters in pig skeletal muscles during postnatal development

et al. 2017

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Effects of low-protein diet and feed restriction on mRNA expression of cationic amino acid transporters in porcine skeletal muscles

Ishida

Ashihara

Nakashima

et al. 2022

Preprint

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We investigated the effects of a low-protein diet and feed restriction on the mRNA expression of cationic amino acid transporters (CATs) in porcine skeletal muscles, specifically, the longissimus dorsi (LD), rhomboideus (RH), and biceps femoris (BF) muscles. Eighteen piglets were randomly divided into three groups: a control (CP21%, ad libitum), low-protein diet (LP, CP16%, ad libitum), and feed-restricted diet (FR, CP21%, 76% feed intake of control pigs) groups. The expression levels of CAT-1 in the LD and BF muscles of LP pigs were higher than that of control pigs, whereas that of FR pigs did not differ from that of control pigs. The CAT-2A expression levels in the RH muscle of FR pigs was higher than that of control pigs. The concentrations of free lysine in all muscles of LP and FR pigs were lower than that of control pigs. The concentrations of free arginine in the LD and RH muscles of LP and FR pigs were lower than that of control pigs. To examine the factors involved in the regulation of CATs mRNA expression, we evaluated the in vitro effects of lysine, arginine, insulin-like growth factor-1, and dexamethasone on the expression of CAT-1, CAT-2A, and CAT-2B in C2C12 myotubes. CAT-1 expression levels increased in response to lysine and/or arginine deprivation. We show, for the first time, that CAT-1 and CAT-2 expression levels in skeletal muscles differ in response to dietary treatments and CAT-1 expression in skeletal muscles appears to increase in response to low free lysine concentrations.

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Molecular Cloning, Tissue Distribution and Expression of Porcine y+L Amino Acid Transporter-1

AiMin¹,

Zhou²,

Zuo³

et al. 2009

Asian Australas. J. Anim. Sci

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In this study, we cloned, sequenced and characterized porcine y+L Amino Acid Transporter-1 (y+LAT1). By screening a translated EST database with the protein sequence of the human y + LAT1 and by using rapid amplification of cDNA ends (RACE), the full-length cDNA encoding porcine y + LAT1 was isolated from porcine intestine RNA. It was 2,111 bp long, encoding a 511 amino acid trans-membrane glycoprotein composed of 12 transmembrane domains. The predicted amino acid sequence was found to be 91%, 90%, 87% and 87% identical to those of cattle, human, mouse and rat y + LAT1 respectively. Real-time RT-PCR results indicated that the small intestine had the highest y + LAT1 mRNA abundance and the lung had the lowest y + LAT1 mRNA abundance. Baby hamster kidney (BHK) cells transfected with green fluorescent protein (GFP) tagged porcine y + LAT1 cDNA indicated that the cellular localization of the gene product in BHK was on the plasma membrane.

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Molecular Cloning, Segmental Distribution and Ontogenetic Regulation of Cationic Amino Acid Transporter 2 in Pigs

Cited by 3 publications

References 22 publications

Expression of cationic amino acid transporters in pig skeletal muscles during postnatal development

Expression of cationic amino acid transporters in pig skeletal muscles during postnatal development

Effects of low-protein diet and feed restriction on mRNA expression of cationic amino acid transporters in porcine skeletal muscles

Molecular Cloning, Tissue Distribution and Expression of Porcine y+L Amino Acid Transporter-1

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