Molecular cloning, polymorphism, and functional activity of the bovine and water buffalo Mx2 gene promoter region

Babiker, H. A. E.; Saito, Tetsuya; Nakatsu, Yuichiro; Takasuga, S.; Morita, Mitsuo; Sugimoto, Yoshikazu; Ueda, Junji; Watanabe, Tomomasa

doi:10.1186/s40064-016-3729-5

Cited by 4 publications

(3 citation statements)

References 44 publications

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“…BAMBI gene was cloned into PMD-18-T vector (Takara Biotechnology Co., Ltd., Dalian, China) and sequenced to identify its sequence according to previous report ( 28 ). BAMBI gene was subsequently cloned into eukaryotic expression vector pCMVp-NEO-BAN (pBAMBI; Takara Biotechnology Co., Ltd.) to generate BAMBI-overexpressed BGC-823 cells.…”

Section: Methodsmentioning

confidence: 99%

Bone morphogenetic protein and activin membrane‑bound inhibitor overexpression inhibits gastric tumor cell invasion via the transforming growth factor‑β/epithelial‑mesenchymal transition signaling pathway

et al. 2018

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Gastric carcinoma is one of the most common human malignancies and remains the second leading cause of cancer-associated mortality worldwide. Gastric carcinoma is characterized by early-stage metastasis and is typically diagnosed in the advanced stage. Previous results have indicated that bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI) overexpression has been demonstrated to inhibit growth and metastasis of gastric cancer cells. However, the molecular mechanisms of the BAMBI-mediated signaling pathway in the progression of gastric cancer are poorly understood. In the present study, to assess whether BAMBI overexpression inhibited the growth and aggressiveness of gastric carcinoma cells through regulation of transforming growth factor (TGF)-β/epithelial-mesenchymal transition (EMT) signaling pathway, the growth and metastasis of gastric carcinoma cells were analyzed following BAMBI overexpression and knockdown in vitro and in vivo. Molecular changes in the TGF-β/EMT signaling pathway were studied in gastric carcinoma cells following BAMBI overexpression and knockdown. DNA methylation of the gene regions encoding the TGF-β/EMT signaling pathway was investigated in gastric carcinoma cells. Tumor growth in tumor-bearing mice was analyzed after mice were subjected to endogenous overexpression of BAMBI. Results indicated that BAMBI overexpression significantly inhibited gastric carcinoma cell growth and aggressiveness, whereas knockdown of BAMBI significantly promoted its growth and metastasis compared with the control (P<0.01). The TGF-β/EMT signaling pathway was downregulated in BAMBI-overexpressed gastric carcinoma cells; however, signaling was promoted following BAMBI knockdown. In addition, it was observed that BAMBI overexpression significantly downregulated the DNA methylation of the gene regions encoding the TGF-β/EMT signaling pathway (P<0.01). Furthermore, RNA interference-mediated BAMBI overexpression also promoted apoptosis in gastric cancer cells and significantly inhibited growth of gastric tumors in murine xenografts (P<0.01). In conclusion, the present findings suggest that BAMBI overexpression inhibited the TGF-β/EMT signaling pathway and suppressed the invasiveness of gastric tumors, suggesting BAMBI may be a potential target for the treatment of gastric carcinoma via regulation of the TGF-β/EMT signaling pathway.

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Section: Methodsmentioning

confidence: 99%

Bone morphogenetic protein and activin membrane‑bound inhibitor overexpression inhibits gastric tumor cell invasion via the transforming growth factor‑β/epithelial‑mesenchymal transition signaling pathway

et al. 2018

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“…During early pregnancy in cattle, conceptus IFNT induces MX2 expression in circulating PBL and may be used to diagnose pregnancy [64,66]. The bovine MX2 promoter region contains multiple ISREs which would explain why MX2 is highly expressed in bovine tissues in response to type I IFNs [67].…”

Section: Ifnt Dependent Epithelial and Stroma Fibroblast Degsmentioning

confidence: 99%

Conceptus-induced, interferon tau-dependent gene expression in bovine endometrial epithelial and stromal cells†

Chaney

Grose

Charpigny

et al. 2020

Biology of Reproduction

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Bovine endometrium consists of epithelial and stromal cells that respond to conceptus interferon tau (IFNT), the maternal recognition of pregnancy (MRP) signal, by increasing expression of IFN-stimulated genes (ISGs). Endometrial epithelial and stromal-cell-specific ISGs are largely unknown but hypothesized to have essential functions during pregnancy establishment. Bovine endometrial epithelial cells were cultured in inserts above stromal fibroblast (SF) cells for 6 h in medium alone or with IFNT. The epithelial and SF transcriptomic response was analyzed separately using RNA sequencing and compared to a list of 369 DEGs recently identified in intact bovine endometrium in response to elongating bovine conceptuses and IFNT. Bovine endometrial epithelial and SF shared 223 and 70 DEGs in common with the list of 369 endometrial DEGs. Well-known ISGs identified in the epithelial and SF were ISG15, MX1, MX2, and OAS2. DEGs identified in the epithelial but not SF included a number of IRF molecules (IRF1, IRF2, IRF3, and IRF8), mitochondria SLC transporters (SLC25A19, SLC25A28, and SLC25A30), and a ghrelin receptor. Expression of ZC3HAV1, an anti-retroviral gene, increased specifically within the SF. Gene ontology analysis identified the type I IFN signaling pathway and activation of nuclear factor kappa B transcription factors as biological processes associated with the epithelial cell DEGs. This study has identified biologically relevant IFNT-stimulated genes within specific endometrial cell types. The findings provide critical information regarding the effects of conceptus IFNT on specific endometrial compartments during early developmental processes in cattle.

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“…However, considering that the IFN promoter sequences in human and trout are different [27,[41][42][43], and since EPC cells were grown at 15°C, while HeLa and FHs74 cells were grown at 37°C, one possibility could be that temperature-dependent conformation of the Mx promoter region interfered with activation of transcription in human cell lines... Although there are currently no concerns about consumers eating DNA-vaccinated fish each vaccine construct will be evaluated on an individual basis [44] and the lack of activity of the trout Mx promoter in human cells could make it attractive for some fish DNA vaccines.…”

Section: Evaluation Of the Lavrmentioning

confidence: 99%

Time-course study of the protection induced by an interferon-inducible DNA vaccine against viral haemorrhagic septicaemia in rainbow trout

Sepúlveda

Lorenzen

Rasmussen

et al. 2019

Fish & Shellfish Immunology

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The highly effective DNA vaccines against diseases caused by fish rhabdoviruses in farmed fish consist of a DNA plasmid vector encoding the viral glycoprotein under the control of a constitutive cytomegalovirus promoter (CMV). Among others, attempts to improve efficacy and safety of these DNA vaccines have focused on regulatory elements of plasmid vectors, which play a major role in controlling expression levels of vaccine antigens. Depending on the context, use of a fish-derived promoter with minimal activity in mammalian cells could be preferable. Another aspect related to the CMV promoter is that constitutive expression of the vaccine antigen may lead to rapid elimination of antigen expressing cells in the fish and thereby potentially reduce the long-term effects of the vaccine. In this study, we compared DNA vaccines with the interferon-inducible Mx promoter from rainbow trout and the CMV promoter, respectively. Plasmid constructs encoding the enhanced green fluorescent protein (EGFP) were used for the in vitro analysis, whereas DNA vaccines encoding the glycoprotein (G) of the viral haemorrhagic septicaemia virus (VHSV) were applied for the in vivo examination. The in vitro analysis showed that while the DNA vaccine with the CMV promoter constitutively drove the expression of EGFP in both fish and human cell lines, the DNA vaccine with the Mx promoter inducibly enhanced the expression of EGFP in the fish cell line. To address the impact on protection, a time-course model was followed as suggested by Kurath et al. (2006), where vaccinated fish were challenged with VHSV at 2, 8 and 78 weeks post-vaccination (wpv). The DNA vaccine with the CMV promoter protected at all times, while vaccination with the DNA vaccine containing the Mx promoter only protected the fish at 8 wpv. However, following induction with Poly (I:C) one week before the challenge, high protection was also evident at 2 wpv. In conclusion, the results revealed a more fish host dependent activity of the trout Mx promoter compared to the traditionally used cross species-active CMV promoter, but improvements will be needed for its application in DNA vaccines to ensure long term protection.

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Molecular cloning, polymorphism, and functional activity of the bovine and water buffalo Mx2 gene promoter region

Cited by 4 publications

References 44 publications

Bone morphogenetic protein and activin membrane‑bound inhibitor overexpression inhibits gastric tumor cell invasion via the transforming growth factor‑β/epithelial‑mesenchymal transition signaling pathway

Bone morphogenetic protein and activin membrane‑bound inhibitor overexpression inhibits gastric tumor cell invasion via the transforming growth factor‑β/epithelial‑mesenchymal transition signaling pathway

Conceptus-induced, interferon tau-dependent gene expression in bovine endometrial epithelial and stromal cells†

Time-course study of the protection induced by an interferon-inducible DNA vaccine against viral haemorrhagic septicaemia in rainbow trout

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