Molecular cloning of the estrogen and progesterone receptors of the American alligator

Katsu, Yoshinao; Bermudez, Dieldrich S.; Braun, Edward L.; Helbing, Caren C.; Miyagawa, Shinichi; Gunderson, Mark P.; Kohno, Satomi; Bryan, Teresa A.; Guillette, Louis J.; Iguchi, Taisen

doi:10.1016/j.ygcen.2003.11.008

Cited by 70 publications

(36 citation statements)

References 56 publications

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“…Phylogenetic studies of nuclear and mitochondrial genes have supported the hypothesis that birds are more closely related to reptilian species than to other vertebrates (Cao et al, 2000;Katsu et al, 2006;Marquez et al, 2011). ERa and ERb sequences of the American alligator clustered within a branch of a single clade comprising birds and crocodilians, while mammals clustered within another branch of this clade (Katsu et al, 2004). Our results further support the hypothesis that pigeons are a sister group to crocodilians and birds.…”

Section: Discussionsupporting

confidence: 83%

Molecular cloning, expression, and regulation of estrogen receptors in pigeon oviduct epithelial cells

Zhang¹,

Chen²,

Li³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Estrogen regulates reproductive behavior and drives the proliferation and differentiation of several cell types. These physiological functions of estrogen are mediated by estrogen receptors (ERs), and each ER isoform plays a distinct role. To clarify the molecular mechanism of estrogen action and to evaluate the effect of ERs on the secretion of ovalbumin (OVA) in pigeon oviduct epithelial cells (POECs), we determined the complete coding sequences encoding ER alpha (ERα) and ER beta (ERβ) in pigeons. The abundance of pigeon ERα and ERβ mRNA was detected using quantitative polymerase chain reaction. These results revealed that pigeon ERα is highly expressed in the oviduct, while pigeon ERb is highly expressed in the ovary and kidney. We hypothesize that ERα mRNA predominates over that of ERβ in the oviduct. The expression of ERα can be down- 1927©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (1): 1926-1937 (2014 Characterization and regulation of pigeon ERs regulated by 17β-estradiol, and the knockdown of ERα promoted OVA mRNA expression in cultured POECs, indicating that ERα may play an important role in OVA secretion.

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Section: Discussionsupporting

confidence: 83%

Molecular cloning, expression, and regulation of estrogen receptors in pigeon oviduct epithelial cells

Zhang¹,

Chen²,

Li³

et al. 2014

Genet. Mol. Res.

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“…Endogenous or exogenous estrogen would generate molecular and cellular action through ERs which belong to the nuclear receptor super-family and act as transcription factors. Like birds and mammals, alligators exhibit 2 forms of the nuclear ER, ER ␣ (ESR1) and ER ␤ (ESR2), which show high sequence similarity to ERs found in other crocodilians, birds, and mammals [Katsu et al, 2004[Katsu et al, , 2006Naidoo et al, 2008]. Extensive research on the molecular action of the steroid hormone receptors, including the ERs, indicates that these receptors are part of a transcription complex along with a number of chaperones and cofactors, including the heat shock proteins (HSPs) [Picard, 2006].…”

mentioning

confidence: 99%

Potential Contributions of Heat Shock Proteins to Temperature-Dependent Sex Determination in the American Alligator

Kohno

Katsu

Urushitani

et al. 2009

Sex Dev

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Sex determination in the American alligator depends on the incubation temperature experienced during a thermo-sensitive period (TSP), although sex determination can be ‘reversed’ by embryonic exposure to an estrogenic compound. Thus, temperature and estrogenic signals play essential roles during temperature-dependent sex determination (TSD). The genetic basis for TSD is poorly understood, although previous studies observed that many of the genes associated with genetic sex determination (GSD) are expressed in species with TSD. Heat shock proteins (HSPs), good candidates because of their temperature-sensitive expression, have not been examined in regard to TSD but HSPs have the ability to modify steroid receptor function. A number of HSP cDNAs (HSP27, DNAJ, HSP40, HSP47, HSP60, HSP70A, HSP70B, HSP70C, HSP75, HSP90α, HSP90β, and HSP108) as well as cold-inducible RNA binding protein (CIRBP) and HSP-binding protein (HSPBP) were cloned, and expression of their mRNA in the gonadal-adrenal-mesonephros complex (GAM) was investigated. Embryonic and neonatal GAMs exhibited mRNA for all of the HSPs examined during and after the TSP. One-month-old GAMs were separated into 3 portions (gonad, adrenal gland, and mesonephros), and sexual dimorphism in the mRNA expression of gonadal HSP27 (male > female), gonadal HSP70A (male < female), and adrenal HSP90α (male > female) was observed. These findings provide new insights on TSD and suggest that further studies examining the role of HSPs during gonadal development are needed.

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“…Quantitative real-time PCR (Q-PCR) has been used to measure mRNA expression in the American alligator [Katsu et al, 2004;Gunderson et al, 2006;Kohno et al, 2008]. Table 1 reports primer sequence information, annealing temperatures, and accession numbers.…”

Section: Methodsmentioning

confidence: 99%

Gonadal mRNA Expression Levels of TGFβ Superfamily Signaling Factors Correspond with Post-Hatching Morphological Development in American Alligators

Moore

Hamlin

Botteri

et al. 2010

Sex Dev

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Paracrine factor signaling regulates many aspects of vertebrate gonadal development. We investigated key ovarian and testicular morphological markers of the American alligator (Alligator mississippiensis) during the first 5 months post-hatching and correlated gonadal development with mRNA expression levels of a suite of regulatory factors. In both sexes, we observed significant morphology changes, including ovarian follicle assembly and meiotic progression of testicular germ cells. Concomitant with these changes were sexually dimorphic and ontogenetically variable mRNA expressions. In ovaries, FOXL2, aromatase, and follistatin mRNA expression was greater than in testes at all ages. At one week after hatching, we observed ovarian medullary remodeling in association with elevated activin/inhibin βA subunit, follistatin, and aromatase mRNA expressions. Three and 5 months following hatching and concomitant with follicle assembly, ovaries showed increased mRNA expression levels of GDF9 and the mitotic factor PCNA. In testes, the activin/inhibin α and βB subunit transcript levels were greater than in ovaries at all ages. Elevated testicular expression of GDF9 mRNA levels at 5 months after hatching aligned with increased spermatogenic activity. We propose that the mRNA expression levels and concomitant morphological changes observed here affect the establishment of alligator reproductive health and later fertility.

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Molecular cloning of the estrogen and progesterone receptors of the American alligator

Cited by 70 publications

References 56 publications

Molecular cloning, expression, and regulation of estrogen receptors in pigeon oviduct epithelial cells

Molecular cloning, expression, and regulation of estrogen receptors in pigeon oviduct epithelial cells

Potential Contributions of Heat Shock Proteins to Temperature-Dependent Sex Determination in the American Alligator

Gonadal mRNA Expression Levels of TGFβ Superfamily Signaling Factors Correspond with Post-Hatching Morphological Development in American Alligators

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