Molecular cloning of serine proteinases from Bothrops jararaca venom gland

Saguchi, Ken-ichi; Hagiwara-Saguchi, Yuki; Murayama, Nobuhiro; Ohi, Hiroshi; Fujita, Yoshiaki; Camargo, Antônio Carlos Martins de; Serrano, Solange M.T.; Higuchi, Shigesada

doi:10.1016/j.toxicon.2005.03.011

Cited by 21 publications

(16 citation statements)

References 47 publications

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“…The C. durissus terrificus venom is composed of amino acids, small peptides, carbohydrates, lipids, biogenic amines, and enzymes (Bercovici et al 1987). B. jararaca venom also is a complex mixture of substances, including peptides, serine, and metalloproteases (Hayashi and Camargo 2005;Saguchi et al 2005). Thus, the different effect on the Giardia trophozoites can be associated to the concentration of some or one of their components.…”

Section: Discussionmentioning

confidence: 99%

In vitro effects of Crotalus durissus terrificus and Bothrops jararaca venoms on Giardia duodenalis trophozoites

et al. 2005

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Considering the snake venoms' pharmacological properties and chemotherapeutic potential as well as the need for new alternatives for Giardia infection treatment, the present study was carried out aiming to evaluate the in vitro effects of crude Crotalus durissus terrificus and Bothrops jararaca venoms on the growth and adherence of Giardia duodenalis trophozoites. Trophozoites (10(6)) were exposed to serial twofold dilutions of C. durissus terrificus and B. jararaca venoms that ranged from 3.125 to 200 microg/ml and from 5 to 320 microg/ml, respectively. The two venoms inhibited the growth of trophozoites, and the level of inhibition varied according to the assayed concentrations and incubation times. The highest reduction of parasite growth was observed with increasing concentrations of the venoms. No effect on parasite adherence was observed. Light microscope observations revealed changes of the pear-shape aspect of the cell and reduction of flagellar beating frequency in the great part of the trophozoites. This is the first attempt to evaluate the in vitro effects of snake venoms on Giardia trophozoites. The findings stress the need for more investigations and prompt us to perform further studies to identify active principles with antigiardial effect.

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Section: Discussionmentioning

confidence: 99%

In vitro effects of Crotalus durissus terrificus and Bothrops jararaca venoms on Giardia duodenalis trophozoites

et al. 2005

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“…In fact, snake venom proteome variability illustrates the central role that diet has played in the adaptation of snakes to their habitats. Moreover, the presence in a specific venom of a diversity of toxins of the same family that maintain a basic structural scaffold but differ from each other in their amino acid sequences and pharmacological activities reflects the accelerated evolution of snake venom toxins [47][48][49][50]. Venom variation is relevant to both basic venom research as well as to the management of snake envenoming.…”

Section: Applications Of Snake Venom Proteomics: From Taxonomic and Omentioning

confidence: 99%

Exploring snake venom proteomes: multifaceted analyses for complex toxin mixtures

2008

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Snake venom proteomes are complex mixtures of a large number of distinct proteins. In a sense, the field of snake venom proteomics has been under investigation since the very earliest biochemical studies on venoms where peptides and proteins were isolated and structurally and biologically characterized. With the recent developments in mass spectrometry for the identification of proteins, coupled with venom gland transcriptomes, has the field of snake venom proteomics began to flourish. These developments have led to exciting insights into the protein composition of venoms and subsequently their pathological activities. In this review, we will discuss the state of art of snake venom proteomics. Although we have not reached the ultimate goal of characterizing and quantifying all unique proteins in a venom proteome, current technologies have opened many opportunities for high-throughput proteomic studies that have gone beyond simple protein identification to analyzing various functional aspects, such as post-translational modifications, proteolytic processing and toxin-target interactions. In this review, we will discuss the technological approaches used in the study of venom proteomics highlighting the advances made and future directions.

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“…Snake venom serine proteases and their complexes have evolved by gene duplication and accelerated evolution [67,68] to activate 'critical' proteins and interfere in the coagulation cascade. They contribute to venom toxicity.…”

Section: Summary and Future Prospectsmentioning

confidence: 99%

Serine Proteases Affecting Blood Coagulation and Fibrinolysis from Snake Venoms

Kini

2005

Pathophysiol Haemos Thromb

157

106

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Snake venom proteases, in addition to their contribution to the digestion of the prey, affect various physiological functions. They affect platelet aggregation, blood coagulation, fibrinolysis, complement system, blood pressure and nervous system. This review provides a ready reference for serine proteases that interfere in blood coagulation and fibrinolysis. They exhibit their activity by activation of specific zymogens of coagulation factors. These serine proteases serve as tools to study molecular details in the activation of specific factors involved in coagulation and fibrinolytic cascades and are useful in treating various thrombotic and hemostatic conditions.

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Molecular cloning of serine proteinases from Bothrops jararaca venom gland

Cited by 21 publications

References 47 publications

In vitro effects of Crotalus durissus terrificus and Bothrops jararaca venoms on Giardia duodenalis trophozoites

In vitro effects of Crotalus durissus terrificus and Bothrops jararaca venoms on Giardia duodenalis trophozoites

Exploring snake venom proteomes: multifaceted analyses for complex toxin mixtures

Serine Proteases Affecting Blood Coagulation and Fibrinolysis from Snake Venoms

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