Molecular Cloning of Peroxinectin Gene and Its Expression in Response to Peptidoglycan and<i>Vibrio Harveyi</i>in Indian White Shrimp<i>Fenneropenaeus Indicus</i>

Shanthi, S.; Manju, S.; Rajakumaran, Perumal; Vaseeharan, Baskaralingam

doi:10.3109/15419061.2014.943396

Cited by 12 publications

(3 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the immunity-related genes, proPO is an important constituent of the proPO cascade reaction that plays an important role in invertebrate protection against invading pathogens by melanization of pathogens (70, 71). Peroxinectin (PXN) is another defense-related gene and is a cell adhesive protein known to be strongly associated with the proPO system (72). It regulates the expression of antimicrobial peptides (AMPs) in invertebrates (73, 74).…”

Section: Discussionmentioning

confidence: 99%

Phloroglucinol Treatment Induces Transgenerational Epigenetic Inherited Resistance Against Vibrio Infections and Thermal Stress in a Brine Shrimp (Artemia franciscana) Model

et al. 2019

View full text Add to dashboard Cite

Emerging, infectious diseases in shrimp like acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio parahaemolyticus and mortality caused by other Vibrio species such as Vibrio harveyi are worldwide related to huge economic losses in industrial shrimp production. As a strategy to prevent disease outbreaks, a plant-based phenolic compound could be used as a biocontrol agent. Here, using the brine shrimp (Artemia franciscana) as a model system, we showed that phloroglucinol treatment of the parental animals at early life stages resulted in transgenerational inherited increased resistance in their progeny against biotic stress, i.e., bacteria (V. parahaemolyticus AHPND strain and V. harveyi) and abiotic stress, i.e., lethal heat shock. Increased resistance was recorded in three subsequent generations. Innate immune-related gene expression profiles and potential epigenetic mechanisms were studied to discover the underlying protective mechanisms. Our results showed that phloroglucinol treatment of the brine shrimp parents significantly (P < 0.05) enhanced the expression of a core set of innate immune genes (DSCAM, proPO, PXN, HSP90, HSP70, and LGBP) in subsequent generations. We also demonstrated that epigenetic mechanisms such as DNA methylation, m6A RNA methylation, and histone acetylation and methylation (active chromatin marker i.e., H3K4Me3, H3K4me1, H3K27me1, H3 hyperacetylation, H3K14ac and repression marker, i.e., H3K27me3, H4 hypoacetylation) might play a role in regulation of gene expression leading toward the observed transgenerational inheritance of the resistant brine shrimp progenies. To our knowledge, this is the first report on transgenerational inheritance of a compound-induced robust protected phenotype in brine shrimp, particularly protected against AHPND caused by V. parahaemolyticus and vibriosis caused by V. harveyi. Results showed that epigenetic reprogramming is likely to play a role in the underlying mechanism.

show abstract

Section: Discussionmentioning

confidence: 99%

Phloroglucinol Treatment Induces Transgenerational Epigenetic Inherited Resistance Against Vibrio Infections and Thermal Stress in a Brine Shrimp (Artemia franciscana) Model

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The resulting hemocyte pellet was used for total RNA isolation by following the method of TRI‐Reagent (Invitrogen). The full length sequence of Fein ‐PX gene was determined by degenerated primer designing, reverse transcriptase‐PCR, cloning and sequencing of overlapping PCR, and rapid amplification of cDNA ends method (Shanthi et al , ). The Fein ‐PX gene sequence was submitted to NCBI GenBank with accession no.…”

Section: Methodsmentioning

confidence: 99%

In vitroandin silicostudies on cell adhesion protein peroxinectin fromFenneropenaeus indicusand screening of heme blockers against activity

2015

Self Cite

View full text Add to dashboard Cite

In invertebrates, the prophenoloxidase (proPO) pathway is involved in the phenol-like antioxidant production against invading pathogens. Overproduction of melanin and phenolic substances leads to the disruption of hemocytes (own host cells); therefore, there is a prerequisite to regulate the antioxidant production, which is performed by the proteases and proPO-associated cell adhesion protein peroxinectin (PX). PX is a macromolecular structure consisting of protein involved in the proPO pathway, which is a potential target in the regulatory mechanism in crustaceans. In the proPO cascade, pattern recognition proteins initiate the proPO cascade by the consequent reaction, and PX is involved in the key step in the regulatory mechanism of phenoloxidase enzyme synthesis. In the present study, Indian white shrimp Fenneropenaeus indicus PX (Fein-PX) gene sequence was used. Upregulation of Fein-PX was determined using immunostimulants β-glucan (agonists) and examined its expression by quantitative RT-PCR. To find the downregulation or negative regulation of Fein-PX, inhibitors were screened, and its 3D model provides molecular insights into the rationale inhibitor design for developing an effective molecule against Fein-PX.

show abstract

“…The presence of integrin binding motif (LDV) in AsHPX2 protein suggested its peroxinectin-like nature. Peroxinectins are invertebrate peroxidases that are involved in cell adhesion (Liu et al, 2004;Vizzini et al, 2013;Shanthi et al, 2014). Interestingly, the surface orientation of LDV motif in AsHPX2 protein seems to be facilitating its adhesion in a way similar to other adhesion molecules (Johanssen et al, 1999;Liu et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Heme-Peroxidase 2, a Peroxinectin-Like Gene, Regulates Bacterial Homeostasis in Anopheles stephensi Midgut

2020

View full text Add to dashboard Cite

The dynamic nature of mosquito gut microbiome is associated with different stages of development and feeding behaviors. Therefore, mosquito gut harbors a wide range of endogenous microbes that promote numerous life processes such as, nutrition, reproduction and immunity. In addition, gut microbiota also play an important role in the regulation of Plasmodium (malaria parasite) development. Thus, understanding the mechanism of microbial homeostasis in mosquito gut might be one of the strategies to manipulate malaria parasite development. In the present study, we characterized a 692 amino acids long secreted midgut heme-peroxidase 2 (AsHPX2) in Anopheles stephensi, the major Indian malaria vector. The presence of putative integrin binding motifs, LDV (Leu-Asp-Val), indicated its peroxinectin-like nature. Our phylogenetic analysis revealed that AsHPX2 is a Culicinae lineage-specific gene. RNA interference (RNAi)-mediated silencing of AsHPX2 gene significantly enhanced the growth of midgut bacteria in sugar-fed mosquitoes against sham-treated controls. Interestingly, bloodfeeding drastically reduced AsHPX2 gene expression and enhanced the growth of midgut bacteria. These results revealed a negative correlation between the expression of AsHPX2 gene and gut bacterial growth. We proposed that AsHPX2, being a mosquitospecific gene, might serve as a "potent target" to manipulate midgut microbiota and vector competence.

show abstract

Molecular Cloning of Peroxinectin Gene and Its Expression in Response to Peptidoglycan andVibrio Harveyiin Indian White ShrimpFenneropenaeus Indicus

Cited by 12 publications

References 32 publications

Phloroglucinol Treatment Induces Transgenerational Epigenetic Inherited Resistance Against Vibrio Infections and Thermal Stress in a Brine Shrimp (Artemia franciscana) Model

Phloroglucinol Treatment Induces Transgenerational Epigenetic Inherited Resistance Against Vibrio Infections and Thermal Stress in a Brine Shrimp (Artemia franciscana) Model

In vitroandin silicostudies on cell adhesion protein peroxinectin fromFenneropenaeus indicusand screening of heme blockers against activity

Heme-Peroxidase 2, a Peroxinectin-Like Gene, Regulates Bacterial Homeostasis in Anopheles stephensi Midgut

Contact Info

Product

Resources

About