Molecular cloning of heat shock protein 10 (Hsp10) and 60 (Hsp60) cDNAs and their expression analysis under thermal stress in the sea cucumber Apostichopus japonicus

Xu, Dongxue; Sun, Lina; Liu, Shilin; Zhang, Libin; Ru, Xiaoshang; Zhao, Ye; Yang, Hongsheng

doi:10.1016/j.cbpb.2014.03.009

Cited by 36 publications

(33 citation statements)

References 53 publications

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“…A Cpn60 conserved domain and seven Gly-Gly-Met repeats at the C terminus were found in the PmHsp60 sequence, in accordance with previous studies (Xu et al 1997;Sanchez et al 1999). The functional domains of the motif, such as ring oligomerization interfaces and ATP/Mg binding sites, were detected and similar characteristics were found in other species (Xu and Qin 2012;Xu et al 2014). This finding suggested that PmHsp60 belongs to the mitochondrial Hsp60 (mtHsp60) chaperone family.…”

Section: Discussionsupporting

confidence: 75%

“…For instance, Hsp60 was observed to be activated to prevent protein denaturation under heat stress (Martin et al 1992). Xu et al (2014) also found that Hsp60 is involved in the heat shock response in the sea cucumber Apostichopus japonicas. In addition, Hsp60 plays important roles in salinity stress mediation in Portunus trituberculatus (Xu and Qin 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Hsp60 is a homolog of phage growth E large (GroEL) in Escherichia coli (Xu et al 2014). Over the last several decades, multiple Hsp60 functions have been discovered.…”

Section: Introductionmentioning

confidence: 99%

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Characterization and function analysis of Hsp60 and Hsp10 under different acute stresses in black tiger shrimp, Penaeus monodon

Shi

Zhao

et al. 2016

Cell Stress and Chaperones

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Heat shock proteins (Hsps) are a class of highly conserved proteins produced in virtually all living organisms from bacteria to humans. Hsp60 and Hsp10, the most important mitochondrial chaperones, participate in environmental stress responses. In this study, the full-length complementary DNAs (cDNAs) of Hsp60 (PmHsp60) and Hsp10 (PmHsp10) were cloned from Penaeus monodon. Sequence analysis showed that PmHsp60 and PmHsp10 encoded polypeptides of 578 and 102 amino acids, respectively. The expression profiles of PmHsp60 and PmHsp10 were detected in the gills and hepatopancreas of the shrimps under pH challenge, osmotic stress, and heavy metal exposure, and results suggested that PmHsp60 and PmHsp10 were involved in the responses to these stimuli. ATPase and chaperone activity assay indicated that PmHsp60 could slow down protein denaturation and that Hsp60/Hsp10 may be combined to produce a chaperone complex with effective chaperone and ATPase activities. Overall, this study provides useful information to help further understand the functional mechanisms of the environmental stress responses of Hsp60 and Hsp10 in shrimp.

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Section: Discussionsupporting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

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Characterization and function analysis of Hsp60 and Hsp10 under different acute stresses in black tiger shrimp, Penaeus monodon

Shi

Zhao

et al. 2016

Cell Stress and Chaperones

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“…Hence, we have been targeting the heat shock response in the intestine in our previous studies (Xu, Sun, Liu, Zhang & Yang, 2016a; Xu, Sun, Liu, Zhang, Ru, et al. ). In our previous proteomics analysis, expression of many genes involved in AA, lipid and carbohydrate metabolic pathways was significantly changed under HS in the intestine of A. japonicus (Xu, Sun, Liu, Zhang & Yang, 2016b).…”

Section: Introductionmentioning

confidence: 99%

Carbohydrate and amino acids metabolic response to heat stress in the intestine of the sea cucumberApostichopus japonicus

Zhou

Yang

2017

Aquac Res

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The sea cucumber Apostichopus japonicus is an economic species mainly distributed along the coast of northern China, south‐eastern Russia, Japan, Republic of Korea and Democratic People's Republic of Korea. Aquaculture industry of A. japonicus has been facing severe challenge of high temperature. In this study, we studied the mRNA expression profiles of eight key metabolic enzymes involved in carbohydrate and amino acids metabolism in A. japonicus under heat stress. The expression of hexokinase, pyruvate kinase and malate dehydrogenase showed downregulated response to heat stress, while the expression of glutamate dehydrogenase, alanine transaminase and branched‐chain aminotransferase showed upregulated response. In addition, the expression of lactate dehydrogenase and glutamate synthase showed no significant difference. We also applied 1H NMR‐based metabolomics to investigate metabolic changes in the intestine tissue of A. japonicus under heat stress, the results of which revealed nine increased and 10 decreased metabolites in the heat stress group. These response genes and metabolites have potential to become markers for identifying severity of heat stress. More importantly, our findings suggest significant links between gene expression and metabolites changing, highlighting regulation networks of carbohydrate and amino acid metabolism in heat‐stressed A. japonicus.

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“…A class of ER stress proteins like members from heat shock proteins (HSPs) family serve as molecular chaperones and are induced in response to ER stress and then involved in protein folding and assembly (Kaufman, 1999). As common member of HSPs family, different sizes of HSPs were well-documented in a wide range of organisms Zhao et al, 2011;Xu et al, 2014). However, knowledge on other class of ER stress proteins with the similar conserved domains, such as 78 kDa glucose-regulated protein (GRP78) and protein disulfide isomerase (PDI), remain largely unclear, especially their connection with host immune regulation.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of two novel molecular chaperones in bacterial-challenged Apostichopus japonicus

Wang

Shao

Zhang

et al. 2015

Gene

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Molecular cloning of heat shock protein 10 (Hsp10) and 60 (Hsp60) cDNAs and their expression analysis under thermal stress in the sea cucumber Apostichopus japonicus

Cited by 36 publications

References 53 publications

Characterization and function analysis of Hsp60 and Hsp10 under different acute stresses in black tiger shrimp, Penaeus monodon

Characterization and function analysis of Hsp60 and Hsp10 under different acute stresses in black tiger shrimp, Penaeus monodon

Carbohydrate and amino acids metabolic response to heat stress in the intestine of the sea cucumberApostichopus japonicus

Molecular characterization of two novel molecular chaperones in bacterial-challenged Apostichopus japonicus

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