Molecular cloning of fragments of bacteriophage T4 DNA

Wilson, Geoffrey G.; Tanyashin, V. I.; Murray, Noreen E.

doi:10.1007/bf00283493

Cited by 92 publications

(32 citation statements)

References 33 publications

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“…The results of complementation in vivo strongly suggest that the cloned early T4 genes 46, 45 and 62 of the recombinant phage 2806-17 (Wilson et al, 1977;Spicer et al, 1982) can be efficiently expressed, giving functional products because of T4 promoters present on the cloned fragment. This was shown by the high burst sizes ofT4 amber mutants of these genes, observed when 2806-17 preinfected the homoimmune lysogen, E. coli W3350 (2i21).…”

Section: Discussionmentioning

confidence: 98%

“…2c147 was from Dr A. D. Kaiser, and 2 i21 from Dr K. C. Luk. 2 T4 recombinant phages 2806-17 (Q) and 2806-16 (J), which carry an imm21 region, were from Dr N. E. Murray (Wilson et al, 1977). ).806-17 (Q) (see Fig.…”

Section: Methodsmentioning

confidence: 99%

“…1) contains a 5-6 kb T4 DNA fragment corresponding to the T4 early genes 62, 44, 45 and 46 (Wilson et al, 1977), and possibly the gene regA, as well as parts of genes 43 and 47 (Spicer et al, 1982). 2806-16 (J) is a similar recombinant which harbours a T4 DNA fragment corresponding to the late genes 13 to 20 (Wilson et aL, 1977).…”

Section: Methodsmentioning

confidence: 99%

“…In this study, a 2-T4 recombinant phage, 2806-17, or Q (Wilson et al, 1977), was used as a simplified system for the study of early T4 gene expression. This 2-T4 recombinant phage carries a 5.6 kb EcoRI restriction fragment of T4 DNA, corresponding to genes 62, 44, 45 and 46 (Wilson et al, 1977).…”

Section: Introductionmentioning

confidence: 99%

“…This 2-T4 recombinant phage carries a 5.6 kb EcoRI restriction fragment of T4 DNA, corresponding to genes 62, 44, 45 and 46 (Wilson et al, 1977). We asked whether these T4 genes, cloned into the 2 vector, can be expressed and, if this is the case, whether their transcription is initiated from T4 promoters present in the cloned segment.…”

Section: Introductionmentioning

confidence: 99%

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Expression of T4 Early Genes 62, 44, 45 and 46 in the -T4 Recombinant Phage 806-17

Mark

1985

Journal of General Virology

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SUMMARYA 2-T4 recombinant phage, 2806-17, which carries the T4 early genes 62, 44, 45 and 46, was studied inside a homoimmune lysogen. Under such conditions, gene expression from the ). promoters is represented. Results showed extensive expression of gene 46, and significant expression of genes 62 and 45. The expression of these early T4 genes is presumed to depend on T4 promoters included in the cloned fragment. A new promoter proximal to gene 46 is implicated. The results also indicate that the extent of gene expression, in terms of complementation, increases with the time allowed for expression.

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Section: Discussionmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Expression of T4 Early Genes 62, 44, 45 and 46 in the -T4 Recombinant Phage 806-17

Mark

1985

Journal of General Virology

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Bacteriophage T4 late promoters: mapping 5′ ends of T4 gene 23 mRNAs.

Kassavetis¹,

Geiduschek²

1982

The EMBO Journal

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More than thirty 5′ ends of RNA molecules which transverse the bacteriophage T4 late gene, gene 23, have been mapped by nuclease protection and reverse transcription, to a 1.7 kilobase pair (kbp) region upstream of gene 23. Most of these 5′ ends arise from RNA processing and degradation. Two sites at which RNA synthesis is initiated have been identified by guanylylation of diphosphate‐ or triphosphate‐terminated RNA with vaccinia virion “capping” enzyme and are situated approximately 0.1 and 0.9 kbp upstream of gene 23. At least one other promoter lies more than 1.7 kbp upstream of gene 23, that is, outside of the mapped region.

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Degrees of relatedness of T-even type E. coli phages using different or the same receptors and topology of serologically cross-reacting sites.

et al. 1983

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The relatedness of a series of T‐even like phages which use the Escherichia coli outer membrane protein OmpA as a receptor, and the classical phages T2, T4 and T6 has been investigated. Immunoelectron microscopy and the pattern of phage resistance in bacterial mutants revealed that: (i) phages of this morphology do not necessarily cross‐react serologically; (ii) phages using different receptors may bind heterologous IgG everywhere except to the tip (comprising approximately 10% of one fiber polypeptide) of the long tail fibers; (iii) cross‐reacting OmpA‐specific phages may bind heterologous IgG only to the tip of these fibers: (iv) OmpA‐specific phages not cross‐reacting at the tip of the tail fibers use different receptor sites on the protein. Absence of cross‐reactivity appears to reflect high degrees of dissimilarity. A DNA probe consisting of genes encoding the two most distal tail fiber proteins of T4 detected homologies only in DNA from phages serologically cross‐reacting at this fiber. Even under conditions of low stringency, allowing the formation of stable hybrids with almost 30% base mismatch, no such homologies could be found in serologically unrelated phages. Thus, in the collection of phages examined, there are sets of very similar and very dissimilar tail fiber genes and even of such gene segments.

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Molecular cloning of fragments of bacteriophage T4 DNA

Cited by 92 publications

References 33 publications

Expression of T4 Early Genes 62, 44, 45 and 46 in the -T4 Recombinant Phage 806-17

Expression of T4 Early Genes 62, 44, 45 and 46 in the -T4 Recombinant Phage 806-17

Bacteriophage T4 late promoters: mapping 5′ ends of T4 gene 23 mRNAs.

Degrees of relatedness of T-even type E. coli phages using different or the same receptors and topology of serologically cross-reacting sites.

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