Molecular cloning of ABNORMAL FLORAL ORGANS : a gene required for flower development in Arabidopsis

Kumaran, M.K.; Ye, D.; Yang, Wei‐Cai; Griffith, Megan; Chaudhury, Abed; Sundaresan, Venkatesan

doi:10.1007/s004970050180

Cited by 21 publications

(17 citation statements)

References 20 publications

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“…6C). These structures resemble the thirdwhorl filamentous organs observed in several Arabidopsis mutants including ufo (Wilkinson and Haughn, 1995), afo (Kumaran et al, 1999), and fil (Sawa et al, 1999), and have also been observed in species hybrids (Michaelis, 1954;Malik et al, 1999;Matsuzuwa et al, 1999).…”

Section: Variation For Developmental Traits and Chromosome Assortmentmentioning

confidence: 63%

Arabidopsis Species Hybrids in the Study of Species Differences and Evolution of Amphiploidy in Plants

et al. 2000

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It is estimated that 5 million years of evolution separate Arabidopsis thaliana from its close relative Arabidopsis lyrata. The two taxa differ by many characteristics, and together they exemplify the differentiation of angiosperms into self-fertilizing and cross-fertilizing species as well as annual and perennial species. Despite their disparate life histories, the two species can be crossed to produce viable and vigorous hybrids exhibiting heterotic effects. Although pollen sterile, the hybrids produce viable ovules and were used as female parent in backcrosses to both parental species. The resulting backcross plants exhibited transgressive variation for a number of interesting developmental and growth traits as well as negative nuclear/ cytoplasmic interactions. Moreover, the genesis of a fertile amphidiploid neospecies, apparently by spontaneous somatic doubling in an interspecific hybrid, was observed in the laboratory. The mechanisms responsible for the generation of amphiploids and the subsequent evolution of amphiploid genomes can now be studied through direct observation using the large arsenal of molecular tools available for Arabidopsis.Plant growth and development have traditionally been studied by generating relevant mutations or by analyzing naturally occurring variants within a species. In only a few cases has the tremendous interspecies variation that was generated over the millions of years of evolution been used. In recent years, it has been increasingly recognized that natural variability is a major resource that could complement traditional approaches. Thus, in the model plant Arabidopsis, intraspecific genetic variation has been noted among different geographical isolates, and this variation, which is largely quantitative in nature, is being subjected to analytical methods developed for the analysis of quantitative trait loci in crop plants (for review, see Alonso-Blanco and Koornneef, 2000). However, the enormous store of natural variation that is manifest in interspecies differences has remained largely untapped.Wide crosses and interspecific hybridizations have been used to investigate the genetic basis of complex traits that differentiate varieties within a species as well as related species in several plant families (Doebley et al

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Section: Variation For Developmental Traits and Chromosome Assortmentmentioning

confidence: 63%

Arabidopsis Species Hybrids in the Study of Species Differences and Evolution of Amphiploidy in Plants

et al. 2000

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“…3C) (Chen et al, 1999;Kumaran et al, 1999;Sawa et al, 1999). FIL is a key regulator of adaxial/ abaxial patterning in lateral organs and functions redundantly with other members of the YABBY gene family (Siegfried et al, 1999).…”

Section: Jag Double Mutants With Genes Involved In Lateral Organ Devementioning

confidence: 99%

TheArabidopsis JAGGEDgene encodes a zinc finger protein that promotes leaf tissue development

et al. 2004

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Important goals in understanding leaf development are to identify genes involved in pattern specification, and also genes that translate this information into cell types and tissue structure. Loss-of-function mutations at the JAGGED (JAG) locus result in Arabidopsis plants with abnormally shaped lateral organs including serrated leaves, narrow floral organs, and petals that contain fewer but more elongate cells. jag mutations also suppress bract formation in leafy, apetala1 and apetala2 mutant backgrounds. The JAG gene was identified by map-based cloning to be a member of the zinc finger family of plant transcription factors and encodes a protein similar in structure to SUPERMAN with a single C2H2-type zinc finger, a proline-rich motif and a short leucine-rich repressor motif. JAG mRNA is localized to lateral organ primordia throughout the plant but is not found in the shoot apical meristem. Misexpression of JAG results in leaf fusion and the development of ectopic leaf-like outgrowth from both vegetative and floral tissues. Thus, JAG is necessary for proper lateral organ shape and is sufficient to induce the proliferation of lateral organ tissue.

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“…The YABBY genes encode a newly defined class of putative plant transcription factors (Bowman and Smyth 1999;Kumaran et al 1999;Sawa et al 1999;Siegfried et al 1999). The conserved Cys 2 -Cys 2 putative zinc-binding and HMG-like domains of INO make it a member of the YABBY gene family.…”

Section: Ino Is a Member Of The Yabby Gene Familymentioning

confidence: 99%

mentioning

confidence: 99%

“…A recently described family of Arabidopsis genes, the YABBY genes, encoding putative transcription factors (Bowman and Smyth 1999;Kumaran et al 1999;Sawa et al 1999;Siegfried et al 1999), participate in determination of abaxial identity in a variety of organs. Three members of this family, FILA-MENTOUS FLOWER (FIL), YABBY2 (YAB2), and YAB3, are expressed exclusively in the abaxial sides of cotyledons, leaves, and floral organs (Sawa et al 1999;Siegfried et al 1999).…”

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INNER NO OUTER regulates abaxial- adaxial patterning in Arabidopsis ovules

Villanueva¹,

Broadhvest²,

Hauser³

et al. 1999

Genes & Development

289

405

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Consisting of only four morphological parts, the Arabidopsis ovule is a relatively simple structure that lends itself to the study of genetic regulation of pattern formation and organogenesis in plants (for review, see Gasser and Robinson-Beers 1993;Reiser and Fischer 1993;Angenent and Colombo 1996;Gasser et al. 1998;Schneitz et al. 1998b). Although initiating as a radially symmetrical primordium, the developing ovule subsequently exhibits differences between the side toward the apex of the carpel (adaxial) and the side toward the base of the carpel (abaxial)-resulting in a bilaterally symmetrical structure. Asymmetric development is most apparent in the outer integument (one of two sheathing structures) and in the funiculus (supporting stalk). The outer integument grows extensively only on the abaxial side of the ovule, and the funiculus curves in the abaxial direction. Two genetic loci involved in asymmetric development of the outer integument have been described. In addition to effects on flower development (Schultz et al. 1991;Bowman et al. 1992), the SUPERMAN (SUP) gene is essential for suppressing adaxial growth of the outer integument (Gaiser et al. 1995), and mutations in this gene lead to nearly equal outer integument growth on both sides of the ovule. In contrast, inner no outer (ino) mutations can lead to an absence of outer integument growth on both sides of the ovule primordium, implicating INO as a positive regulator of integument growth or a determinant of polarity (Gaiser et al. 1995;Baker et al. 1997;Schneitz et al. 1997).In angiosperms, bilateral symmetry is also a common characteristic of leaves, floral organs, and often whole flowers. In these structures, bilateral symmetry results from significant differences in development between the adaxial (toward the shoot apex) and abaxial (away from the shoot apex) sides. Recently, several genes involved in establishing these abaxial-adaxial patterns have been identified. The cycloidea (cyc) and dichotoma (dich) genes of Antirrhinum majus are expressed on the adaxial sides of flowers, where they specify adaxial floral development (Luo et al. 1996). In contrast, phantastica (phan), which appears to be essential for identity of the adaxial leaf surface in this same species, is expressed throughout the leaf (Waites et al. 1998), and must, therefore, require additional asymmetrically distributed factors for its activity.In Arabidopsis thaliana, vegetative structures of the phabulosa-1d mutant are radially symmetrical, apparently as a result of adaxialization, and PHABULOSA may be one determinant of abaxial cell fate in this species (McConnell and Barton 1998). A recently described family of Arabidopsis genes, the YABBY genes, encoding putative transcription factors (Bowman and Smyth 1999;Kumaran et al. 1999;Sawa et al. 1999;Siegfried et al. 1999), participate in determination of abaxial identity in a variety of organs.

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Molecular cloning of ABNORMAL FLORAL ORGANS : a gene required for flower development in Arabidopsis

Cited by 21 publications

References 20 publications

Arabidopsis Species Hybrids in the Study of Species Differences and Evolution of Amphiploidy in Plants

Arabidopsis Species Hybrids in the Study of Species Differences and Evolution of Amphiploidy in Plants

TheArabidopsis JAGGEDgene encodes a zinc finger protein that promotes leaf tissue development

INNER NO OUTER regulates abaxial- adaxial patterning in Arabidopsis ovules

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