TheArabidopsis JAGGEDgene encodes a zinc finger protein that promotes leaf tissue development

Abstract

Important goals in understanding leaf development are to identify genes involved in pattern specification, and also genes that translate this information into cell types and tissue structure. Loss-of-function mutations at the JAGGED (JAG) locus result in Arabidopsis plants with abnormally shaped lateral organs including serrated leaves, narrow floral organs, and petals that contain fewer but more elongate cells. jag mutations also suppress bract formation in leafy, apetala1 and apetala2 mutant backgrounds. The JAG gene was identified by map-based cloning to be a member of the zinc finger family of plant transcription factors and encodes a protein similar in structure to SUPERMAN with a single C2H2-type zinc finger, a proline-rich motif and a short leucine-rich repressor motif. JAG mRNA is localized to lateral organ primordia throughout the plant but is not found in the shoot apical meristem. Misexpression of JAG results in leaf fusion and the development of ectopic leaf-like outgrowth from both vegetative and floral tissues. Thus, JAG is necessary for proper lateral organ shape and is sufficient to induce the proliferation of lateral organ tissue.

The Arabidopsis ZINC FINGER PROTEIN3 Interferes with Abscisic Acid and Light Signaling in Seed Germination and Plant Development

“…In silico analysis revealed that there are 176 C2H2-type zinc finger proteins in Arabidopsis, from which 33 are conserved in other eukaryotes and 143 appear to be plant specific (Englbrecht et al, 2004). Members of this gene family have been implicated in the regulation of plant development, including photomorphogenesis, leaf, shoot, and flower organogenesis, gametogenesis, seed development, and dormancy (Sakai et al, 1995;Chrispeels et al, 2000;Prigge and Wagner, 2001;Dinkins et al, 2002;He and Gan, 2004;Ohno et al, 2004;Takeda et al, 2004). Our data show that ZFP3 and its closest C2H2-type zinc-finger protein homologs (ZFPs) are negative regulators of ABA signaling during germination, influence vegetative development and fertility, and modulate red light signaling in seedling photomorphogenesis.…”

BLADE-ON-PETIOLE–Dependent Signaling Controls Leaf and Floral Patterning in Arabidopsis

“…Leafy petioles are also caused by the ectopic expression of LEAFY PETIOLE (LEP), which encodes an EREBP/AP2-type transcription factor (van der Graaff et al, 2000), and JAGGED (JAG), which encodes a C 2 H 2 zinc finger transcription factor (Dinneny et al, 2004;Ohno et al, 2004). One possibility is that BOP activity regulates the spatial expression of leaf-promoting transcription factors such as JAG or LEP in the proximal parts of leaves and in floral bracts.…”

“…Haughn, unpublished results). Plants misexpressing JAG also develop floral bracts (Dinneny et al, 2004;Ohno et al, 2004), another aspect of the bop1 bop2 mutant phenotype. Previous analyses of bop1-1 plants have shown that knox genes are also misexpressed in the leaves, predominantly in the petiole region (Ha et al, 2003).…”

Physical Interaction of Floral Organs Controls Petal Morphogenesis in Arabidopsis

“…Petal primordia arise at four loci in the second whorl, and this positioning is established independently of the process that determines organ identity (Griffith et al, 1999;Brewer et al, 2004;Takeda et al, 2004;Xing et al, 2005;Lampugnani et al, 2013). After initiation, the growth of petals depends on the activity of cell division and expansion along the proximal-distal axis, which is partly regulated by JAGGED (Dinneny et al, 2004;Ohno et al, 2004). Final petal size is determined by the balance of cell proliferation and expansion (Mizukami and Fischer, 2000;Szécsi et al, 2006).…”