Molecular Cloning of a Novel Member of the GLUT Family of Transporters, SLC2A10 (GLUT10), Localized on Chromosome 20q13.1: A Candidate Gene for NIDDM Susceptibility

McVie‐Wylie, Alison; Lamson, David R.; Chen, Y.T.

doi:10.1006/geno.2000.6457

Cited by 130 publications

(96 citation statements)

References 17 publications

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“…[18][19][20][21][22] In contrary, while Afamin and Sec14l4 have no known functions in mediating insulin secretion, they have been implicated in transport of α-tocopherol, a form of vitamin E. [23][24][25] Based on their involvement in transport, these proteins may play crucial roles in regulating exocytosis and vesicular transport in islet β-cells. Additionally, with the implicated functions of Slc2a10 (Glut10), a facilitated glucose transporter and Neurogenin3 in maintaining glucose homeostasis, [26][27][28][29][30][31] our study suggest that increased levels of these genes potentially lead to the enhanced β-cell function seen in the Pdx1 PB -Isl-1-Myc transgenic mice. Similar to an increase of Arx expression demonstrated previously by real-time PCR, 11 an increase in MafA expression was also detected (Fig.…”

Section: β-Cell Function In Pdx1mentioning

confidence: 66%

“…3B). From our real-time PCR, we detected an increase in the expression of Afamin, 23 Caps2, 21,22 Slc2a10, 26,27 Sec14l4, 24,25 and P2rx7 [18][19][20] as well as Neurogenin3 28 in the Pdx1 PB -Isl-1-Myc islets (Fig. 3B).…”

Section: β-Cell Function In Pdx1mentioning

confidence: 83%

“…These genes are implicated to function in protein trafficking and metabolism, and may play important roles in β-cell biology. In this analysis, we were able to detect an increase in Slc2a10 (Glut10), a gene that has been implicated in glucose metabolism and type 2 diabetes, 26,27 increased levels of SEC14L4, a factor that has been implicated to be involved in secretion in organs like salivary gland, prostate and pancreas, 24,25 and a 35-fold increase in Afamin levels, a transporter for α-tocopherol in the CNS. 23 Additionally, P2rx7, a purinergic receptor with implicated function for regulating both endocrine and exocrine pancreas function and Caps2, transgenic mouse line was characterized in our study, which we acknowledged it as a caveat, we are confident that the effects we observe in the Pdx1 PB -Isl-1-Myc mice are due to the elevation of Isl-1 expression as Arx and MafA, two genes that are downregulated in Isl-1 deficient pancreata, 10,11 were increased the islets of the Pdx1 PB -Isl-1-Myc transgenic mice.…”

Section: Discussionmentioning

confidence: 94%

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Elevation of transcription factor Islet-1 levels in vivo increases β-cell function but not β-cell mass

Liu

Walp

May

2012

Islets

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Section: β-Cell Function In Pdx1mentioning

confidence: 66%

Section: β-Cell Function In Pdx1mentioning

confidence: 83%

Section: Discussionmentioning

confidence: 94%

See 1 more Smart Citation

Elevation of transcription factor Islet-1 levels in vivo increases β-cell function but not β-cell mass

Liu

Walp

May

2012

Islets

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“…highest levels in the liver and pancreas, but is also expressed in the heart, lung, brain, skeletal muscle, placenta and adipose tissue (Dawson et al, 2001;McVie-Wylie et al, 2001). In vitro, SLC2A10 is expressed by cultured vascular smooth muscle cells (VSMC) and skin fibroblasts, which localize the protein either to the plasma membrane or the perinuclear envelope (Coucke et al, 2006).…”

mentioning

confidence: 99%

Characterization and expression pattern analysis of the facilitative glucose transporter 10 gene (slc2a10) in Danio rerio

Chiarelli

Ritelli²,

Zoppi³

et al. 2011

Int. J. Dev. Biol.

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The SLC2A10 gene located on chromosome 20q13.1 encodes the facilitative glucose transporter 10 (GLUT10), a class III member of the SLC2A facilitative glucose transporter family. Mutations in the human SLC2A10 gene cause arterial tortuosity syndrome (ATS), a rare autosomal recessive connective tissue disorder. In this work, we report the characterization of the slc2a10 ortholog gene in zebrafish (Danio rerio) and its expression pattern during embryonic development and in adult tissues. The slc2a10 gene consists of 5 exons, spanning 8 kb and mapping to a region on chromosome 11 that exhibits conserved synteny with human chromosome 20. The gene encodes Glut10, a 513 amino acid protein that maintains the 12 transmembrane domain structure typical of the GLUTs family, and shares the specific functional motifs involved in sugar transport with the vertebrate GLUT10. RT-PCR analysis showed that two specific splice variants, both including the 5'-UTR region, were expressed during embryogenesis and in different adult zebrafish tissues and organs. In situ hybridization analyses demonstrated a maternal origin of the total slc2a10 mRNA and its ubiquitous distribution until the early somitogenesis stage. In later embryonic stages, slc2a10 mRNA was detected in the otic vesicles, hatching gland cells, pectoral fin, posterior tectum and swim bladder. Overall, these results suggest a wide role of slc2a10 during zebrafish development.

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“…he recently cloned SLC2A10 gene encodes a 541 amino acid putative facilitative glucose transporter (GLUT10) of the GLUT family class III with between 30 and 34% amino acid homology with the known GLUT proteins (1,2). The SLC2A10 gene contains five exons and spans a genomic region of at least 28 kb.…”

mentioning

confidence: 99%

Genetic Variation of the GLUT10 Glucose Transporter (SLC2A10) and Relationships to Type 2 Diabetes and Intermediary Traits

et al. 2003

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The SLC2A10 gene encodes the GLUT10 facilitative glucose transporter, which is expressed in high amounts in liver and pancreas. The gene is mapped to chromosome 20q12-q13.1, a region that has been shown to be linked to type 2 diabetes. The gene was examined in 61 Danish type 2 diabetic patients, and a total of six variants (؊27C3 T, Ala206Thr, Ala272Ala, IVS2 ؉ 10G3 A, IVS4 ؉ 18T3 G, and IVS4 ؉ 26G3 A) were identified and investigated in an association study, which included 503 type 2 diabetic patients and 510 glucose-tolerant control subjects. None of the variants were associated with type 2 diabetes. Interestingly, carriers of the codon 206 Thr allele had 18% lower fasting serum insulin levels (P ‫؍‬ 0.002) and 20% lower insulinogenic index (P ‫؍‬ 0.03) than homozygous carriers of the Ala allele. These results suggest that variation in the coding region of SLC2A10 does not contribute substantially to the pathogenesis of type 2 diabetes in the examined study population. However, the codon 206 polymorphism may be related to the interindividual variation in fasting and oral glucoseinduced serum insulin levels. Diabetes 52:2445-2448, 2003 T he recently cloned SLC2A10 gene encodes a 541 amino acid putative facilitative glucose transporter (GLUT10) of the GLUT family class III with between 30 and 34% amino acid homology with the known GLUT proteins (1,2). The SLC2A10 gene contains five exons and spans a genomic region of at least 28 kb. Northern hybridization analysis indicates highest levels of expression in liver and pancreas (2). When expressed in Xenopus oocytes, human GLUT10 exhibited 2-deoxy-D-glucose transport with an apparent K m of ϳ0.3 mmol/l (3). Given the function of GLUT10 as a member of the GLUT family of facilitative glucose transporters, the tissue distribution, and the fact that SLC2A10 is mapped to a chromosomal region 20q12-q13.1 (spanned by markers D20S119 and D20S197), which in several studies (4 -8) has shown linkage to type 2 diabetes (logarithm of odds score peak is located at D20S195 within SLC2A10), we hypothesized that variation in SLC2A10 may confer increased susceptibility to type 2 diabetes or altered circulating insulin levels. Thus, this study reports the results of a mutation analysis of the coding region of the SLC2A10 gene and the identification of six novel single nucleotide polymorphisms. The mutation screening covered the coding (translated) region of SLC2A10 and an additional 128 bp upstream from the translation initiation site (ATG). In the 61 diabetic patients, we identified a total of six different nucleotide variants: Ϫ27C3 T (relative to the ATG site, identified in 2 of 61 patients), Ala206Thr (nucleotide position 616: GCC3 ACC, 2 patients), Ala272Ala (816: GCC3 GCG, 1 patient), IVS2 ϩ 10G3 A (5 patients), IVS4 ϩ 18T3 G (37 patients), and IVS4 ϩ 26G3 A (1 patient). These variants were further examined in a casecontrol study comprising 503 type 2 diabetic patients and 510 (or 231, see "Subjects") glucose-tolerant subjects. The allele frequencies and genotype distributions o...

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Molecular Cloning of a Novel Member of the GLUT Family of Transporters, SLC2A10 (GLUT10), Localized on Chromosome 20q13.1: A Candidate Gene for NIDDM Susceptibility

Cited by 130 publications

References 17 publications

Elevation of transcription factor Islet-1 levels in vivo increases β-cell function but not β-cell mass

Elevation of transcription factor Islet-1 levels in vivo increases β-cell function but not β-cell mass

Characterization and expression pattern analysis of the facilitative glucose transporter 10 gene (slc2a10) in Danio rerio

Genetic Variation of the GLUT10 Glucose Transporter (SLC2A10) and Relationships to Type 2 Diabetes and Intermediary Traits

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